Results 51 to 60 of about 5,929 (191)
Internal light source for deep photodynamic therapy
Light: Science & Applications, 2022 Photodynamic therapy (PDT) for deep-seated lesion is seriously hindered by the limited depth of visible light penetration. Most recently, researchers have designed a genetically-encoded NanoLuc-miniSOG with internal light source for self-excitation ...
Buhong Li, Li Lin
doaj +1 more source
Illuminating cellular and extracellular vesicle-mediated communication via a split-Nanoluc reporter in vitro and in vivo [PDF]
, 2023 Tools to effectively demonstrate and quantify functional delivery in cellular communication have been lacking. This study reports the use of a fluorescently labeled split Nanoluc reporter system to demonstrate and quantify functional transfer between ...
Broekman, M.L.D. +13 more
core +1 more source
Advanced Science, EarlyView.Engineered extracellular vesicles displaying Ephrin‐B2 selectively target Ephrin‐B4–expressing ovarian cancer cells, enabling precise delivery in patient‐derived models. This scalable bio‐manufacturing platform reveals a versatile strategy to exploit Ephrin signaling for highly specific therapeutic payload delivery and motivates exploration of tailored
Nihar Godbole +17 more
wiley +1 more source
Evidence for the extracellular delivery of influenza NS1 protein
Microbiology Independent Research Journal, 2021 We constructed a reporter influenza A/Puerto Rico/8/1934 virus expressing truncated 124aa N-terminal NS1 protein fused to a luciferase reporter sequence (NanoLuc) without signal peptide.
Anastasia A. Pulkina +4 more
doaj +1 more source
Transposase‐Assisted Donor Tethering Boosts Large‐Fragment HDR in Plants
Advanced Science, EarlyView.A transposase‐assisted donor tethering strategy is developed to enhance homology‐directed repair in plants. By recruiting donor DNA to double‐strand breaks and synergizing with repair pathway reprogramming and transcription‐coupled donor design, this system markedly improves large‐fragment targeted insertion efficiency, providing a robust platform for ...
Sha Wei +8 more
wiley +1 more source
A genetically encoded BRET-based SARS-CoV-2 Mpro protease activity sensor
Communications Chemistry, 2022 The main protease (Mpro) of SARS-CoV-2 is an important target for COVID-19 therapy. Here, a pair of genetically encoded BRET-based sensors for detecting Mpro activity are generated by sandwiching N-terminal autocleavage sites in between the mNeonGreen ...
Anupriya M. Geethakumari +6 more
doaj +1 more source
Antioxidants, 2023 With the rising prevalence of obesity, non-alcoholic fatty liver disease (NAFLD) now affects 20–25% of the global population. NAFLD, a progressive condition associated with oxidative stress, can result in cirrhosis and liver cancer in 10% and 3% of ...
James Li +5 more
doaj +1 more source
Advanced Science, EarlyView.Inhibition of KDELR2 in a small fraction of tumor cells generates sustainable immunogenic cell death conditions within the tumor microenvironment. These conditions promote the regression of tumors in a T cell independent manner. During regression, macrophages prime T cells that subsequently provide systemic protection against recurrence. The potency of
Shakti P Pattanayak +6 more
wiley +1 more source
Engineering new tools and imaging platforms with NanoLuc
, 2022 Bioluminescence imaging is a powerful modality for probing cellular and molecular features in vivo. This technology involves a luciferase enzyme that oxidizes a small molecule luciferin, thereby producing light. The recent development of NanoLuc, a small,
Ng, Kevin King-Yiu
core
A probabilistic view of protein stability, conformational specificity, and design
Scientific Reports, 2023 Various approaches have used neural networks as probabilistic models for the design of protein sequences. These "inverse folding" models employ different objective functions, which come with trade-offs that have not been assessed in detail before.
Jacob A. Stern +8 more
doaj +1 more source