Results 51 to 60 of about 6,439 (202)
Antioxidants, 2023 With the rising prevalence of obesity, non-alcoholic fatty liver disease (NAFLD) now affects 20–25% of the global population. NAFLD, a progressive condition associated with oxidative stress, can result in cirrhosis and liver cancer in 10% and 3% of ...
James Li +5 more
doaj +1 more source
Dual Reporter Bioluminescence Imaging with NanoLuc and Firefly Luciferase [PDF]
, 2018 Bioluminescence imaging is a powerful, broadly utilized method for noninvasive imaging studies in cell-based assays and small animal models of normal physiology and multiple diseases. In combination with molecular engineering of cells and entire organisms using luciferase enzymes, bioluminescence imaging has enabled novel applications including studies
Anne E, Gibbons +2 more
openaire +2 more sources
FEBS Open Bio, EarlyView.Human ABCE1 cannot functionally replace its yeast ortholog. Yeast–human chimera analysis identified NBD1 as a major interspecies barrier. Genetic screening yielded hABCE1 revertants that rescue yeast viability but fail to suppress aberrant translation reinitiation in the 3′ UTR.
Eriko Nakata +3 more
wiley +1 more source
Advanced Healthcare Materials, EarlyView.We developed a patient‐derived, functional microfluidic model of the diffuse midline glioma (DMG) blood–brain–tumor barrier (BBTB) comprised of endothelial cells, astrocytes, pericytes, and tumor cells. The system forms perfusable microvasculature, reveals the BBTB retains vascular integrity, identifies DMG‐specific transcriptomic changes distinct from
Kimberly R. Bennett +7 more
wiley +1 more source
A genetically encoded BRET-based SARS-CoV-2 Mpro protease activity sensor
Communications Chemistry, 2022 The main protease (Mpro) of SARS-CoV-2 is an important target for COVID-19 therapy. Here, a pair of genetically encoded BRET-based sensors for detecting Mpro activity are generated by sandwiching N-terminal autocleavage sites in between the mNeonGreen ...
Anupriya M. Geethakumari +6 more
doaj +1 more source
Evidence for the extracellular delivery of influenza NS1 protein
Microbiology Independent Research Journal, 2021 We constructed a reporter influenza A/Puerto Rico/8/1934 virus expressing truncated 124aa N-terminal NS1 protein fused to a luciferase reporter sequence (NanoLuc) without signal peptide.
Anastasia A. Pulkina +4 more
doaj +1 more source
A probabilistic view of protein stability, conformational specificity, and design
Scientific Reports, 2023 Various approaches have used neural networks as probabilistic models for the design of protein sequences. These "inverse folding" models employ different objective functions, which come with trade-offs that have not been assessed in detail before.
Jacob A. Stern +8 more
doaj +1 more source
Fluorophore-NanoLuc BRET Reporters Enable Sensitive In Vivo Optical Imaging and Flow Cytometry for Monitoring Tumorigenesis [PDF]
, 2015 Fluorescent proteins are widely used to study molecular and cellular events, yet this traditionally relies on delivery of excitation light, which can trigger autofluorescence, photoxicity, and photobleaching, impairing their use in vivo.
Reza, Md Shamim +8 more
core +1 more source
Increasing Lipid Nanoparticle mRNA Copy Number Improves Transfection Potency
Advanced Materials, EarlyView.As lipid nanoparticle (LNP) systems are the leading platform for in vivo nucleic acid delivery, improving transfection potency of these systems is of considerable interest. In this work, we explored a post‐mixing formulation method involving a two‐step buffer exchange that increased the number of mRNA copies within each LNP while reducing the number of
Yao Zhang +12 more
wiley +1 more source
A NanoLuc luciferase-based assay enabling the real-time analysis of protein secretion and injection by bacterial type III secretion systems. [PDF]
, 2020 The elucidation of the molecular mechanisms of secretion through bacterial protein secretion systems is impeded by a shortage of assays to quantitatively assess secretion kinetics.
Bohn, Erwin +13 more
core +1 more source