Results 51 to 60 of about 5,275 (178)

Construction of oncolytic reporter influenza viral vectors and assessment of their safety following intracranial administration in rats

Microbiology Independent Research Journal
INTRODUCTION: Oncolytic viruses are a promising approach for treating malignant brain tumors as part a of combination therapy. OBJECTIVE: To develop reporter influenza A viruses expressing NanoLuc luciferase and evaluate their safety following ...
A. A. Pulkina, A. S. Mustafaeva, E. A. Romanovskaya-Romanko, M. A. Plotnikova, O. O. Ozhereleva, M. A. Shuklina, L. N. Kiseleva, Y. S. Alekseeva, N. S. Kurmazov, A. R. Muslimov, A.-P.S. Shurygina, M. A. Stukova   +11 more
doaj   +1 more source

Programmable Nanobody‐Targeting Chimeras Enable Intracellular Viral Protein Degradation

Advanced Science, EarlyView.
Nab‐TAC enables targeted degradation of HBV surface antigen (HBsAg) in vivo. By fusing nanobody‐based recognition domains with programmable proteasome‐recruiting degradation signals, Nab‐TAC reduces HBsAg levels in a hydrodynamic HBV mouse model. ABSTRACT Chronic hepatitis B virus (HBV) infection remains a major global health challenge, largely because
Max Yu‐Chen Pan, Ting‐Hui Lee, Tzu‐Ning Peng, Eva Yi‐Hsuan Wu, Yu‐Ting Chiu, Chih‐Chien Chiu, Hong Vinh Nguyen, Yuan‐Shao Pao, Ming‐Hong Chao, Richard Kuan‐Lin Lee, Han‐Chung Wu, Lily Hui‐Ching Wang   +11 more
wiley   +1 more source

Plasmid: NK588 (pAct-nanoluc) v1

, 2019
The attached file is the full sequence for a plasmid that uses gene regulatory elements from S. rosetta to drive the expression of nanoluc luciferase upon transfection in S. rosetta. The plasmid is backbone is pUC19 and can be propagated in E. coli with Ampicillin selection.
David Booth, Nicole King
openaire   +1 more source

Revolutionizing Healthcare With Paper‐Based Nucleic Acid Testing

Exploration, EarlyView.
This work summarizes strategies to enhance paper‐based devices for nucleic acid testing. Key approaches include optimizing paper platforms, improving nucleic acid amplification, and refining labeling/signaling methods. These advancements aim to boost sensitivity, speed, and usability, making paper‐based diagnostics more effective for point‐of‐care ...
Hong Zhang, Yi Yang, Heng Li, Xiaolin Hu, Hui Xiao, Dou Wang, Sergio Benardini, Wei Gu, Yang Luo   +8 more
wiley   +1 more source

High-throughput functional analysis provides novel insight into type VII secretion in Staphylococcus aureus

Open Biology
Successful colonization by the opportunistic pathogen Staphylococcus aureus depends on its ability to interact with other microorganisms. Staphylococcus aureus strains harbour a T7b subtype of type VII secretion system (T7SSb), a protein secretion system
Yaping Yang, Aaron A. Scott, Holger Kneuper, Felicity Alcock, Tracy Palmer   +4 more
doaj   +1 more source

An Integrated Approach toward NanoBRET Tracers for Analysis of GPCR Ligand Engagement

Molecules, 2021
Gaining insight into the pharmacology of ligand engagement with G-protein coupled receptors (GPCRs) under biologically relevant conditions is vital to both drug discovery and basic research.
Michael P. Killoran, Sergiy Levin, Michelle E. Boursier, Kristopher Zimmerman, Robin Hurst, Mary P. Hall, Thomas Machleidt, Thomas A. Kirkland, Rachel Friedman Ohana   +8 more
doaj   +1 more source

Hemp (Cannabis sativa L.) Seed Protein and Its Hydrolysate Promote Bone Formation via the SMAD‐Dependent BMP Pathway

iNew Medicine, EarlyView.
ABSTRACT Objective With the global increase in aging populations, the prevalence of osteoporosis is rising. Current preventive and therapeutic strategies remain limited. The present study aimed to investigate the effects of hemp seed protein (HSP) and its hydrolysis‐derived bioactive peptides (HPH) on osteogenic differentiation and to elucidate their ...
Yan Wang, Jiameng Jia, Yang Yu, Kaiyuan Zheng, Meng Deng, Wei Chen, Guoming Liu, Ping Yi, Dongning Ling, Jing Zhang, Linfeng Liu, Bing Yang, Qing Wu, Ping Wei, Chong Yin   +14 more
wiley   +1 more source

Toward Predicting Pandemic Potential: A Comparative Analysis of Virus‐Host Interactions Between Diverse Influenza A Viruses and the Human Innate Immune System

PROTEOMICS, EarlyView.
ABSTRACT Zoonotic infections caused by influenza A viruses (IAVs) of animal origin are a major public health concern, as they may represent the initial step toward the emergence of a pandemic strain. The host range of IAVs is shaped by species‐specific virus‐host interactions, and several host barriers have been identified that restrict the adaptation ...
Antoine Gerodez, Melanie Dos Santos, Mikaël Attia, Killian De Geest, Ibrahim Hamid, Rodrigue Tesse, Ilham Fdillate, Marc Boschmans, Steven Van Borm, Mieke Steensels, Benedicte Lambrecht, Lionel Tafforeau, Caroline Demeret, Cyril Barbezange   +13 more
wiley   +1 more source

Dehydrocostus Lactone Suppresses Hepatocellular Carcinoma by Inhibiting Protein Tyrosine Kinase‐7 Mediated β‐Catenin Signaling

Phytotherapy Research, EarlyView.
Schematic summary of the experimentally supported mechanism of DHL in hepatocellular carcinoma. Dehydrocostus lactone (DHL), a sesquiterpene lactone derived from Aucklandia lappa, directly engages protein tyrosine kinase 7 (PTK7), as supported by molecular docking, CETSA, and DARTS assays.
Xiwen Fan, Junhui Li, Yu Qiao, Yuwen Zhong, Xuecheng Ge, Yaoshuai Zhang, Zishu Wang, Feng Qian, Weixin Ren   +8 more
wiley   +1 more source

Holistic monitoring of Campylobacter jejuni biofilms with NanoLuc bioluminescence

Applied Microbiology and Biotechnology
Campylobacter jejuni, a major cause of foodborne zoonotic infections worldwide, shows a paradoxical ability to survive despite its susceptibility to environmental and food-processing stressors. This resilience is likely due to the bacterium entering a viable but non-culturable state, often within biofilms, or even initiating biofilm formation as a ...
Tjaša Čukajne, Petra Štravs, Orhan Sahin, Qijing Zhang, Aleš Berlec, Anja Klančnik   +5 more
openaire   +3 more sources