Results 71 to 80 of about 5,929 (191)

Toward Predicting Pandemic Potential: A Comparative Analysis of Virus‐Host Interactions Between Diverse Influenza A Viruses and the Human Innate Immune System

PROTEOMICS, EarlyView.
ABSTRACT Zoonotic infections caused by influenza A viruses (IAVs) of animal origin are a major public health concern, as they may represent the initial step toward the emergence of a pandemic strain. The host range of IAVs is shaped by species‐specific virus‐host interactions, and several host barriers have been identified that restrict the adaptation ...
Antoine Gerodez, Melanie Dos Santos, Mikaël Attia, Killian De Geest, Ibrahim Hamid, Rodrigue Tesse, Ilham Fdillate, Marc Boschmans, Steven Van Borm, Mieke Steensels, Benedicte Lambrecht, Lionel Tafforeau, Caroline Demeret, Cyril Barbezange   +13 more
wiley   +1 more source

Exchanging surface glycoproteins has a greater impact on tecVLP titer and NanoLuc signal than exchanging minigenomes.

, 2015
(A) TCID50 titers/mL and NanoLuc signals in relative light units (RLU) measured in SW-13 cells treated with tecVLPs with Afg09 GPC and with L segment NCR minigenomes from indicated CCHFV strains.
Scott D. Pegan (832047), Marko Zivcec (191739), Christina F. Spiropoulou (139312), César G. Albariño (139297), Maureen G. Metcalfe (309827), Éric Bergeron (5663428), Lisa W. Guerrero (832046)   +6 more
core   +1 more source

Dehydrocostus Lactone Suppresses Hepatocellular Carcinoma by Inhibiting Protein Tyrosine Kinase‐7 Mediated β‐Catenin Signaling

Phytotherapy Research, EarlyView.
Schematic summary of the experimentally supported mechanism of DHL in hepatocellular carcinoma. Dehydrocostus lactone (DHL), a sesquiterpene lactone derived from Aucklandia lappa, directly engages protein tyrosine kinase 7 (PTK7), as supported by molecular docking, CETSA, and DARTS assays.
Xiwen Fan, Junhui Li, Yu Qiao, Yuwen Zhong, Xuecheng Ge, Yaoshuai Zhang, Zishu Wang, Feng Qian, Weixin Ren   +8 more
wiley   +1 more source

Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing [PDF]

Nature Communications, 2016
AbstractOptogenetic techniques allow intracellular manipulation of Ca++by illumination of light-absorbing probe molecules such as channelrhodopsins and melanopsins. The consequences of optogenetic stimulation would optimally be recorded by non-invasive optical methods.
Jie Yang, Derrick Cumberbatch, Samuel Centanni, Shu-qun Shi, Danny Winder, Donna Webb, Carl Hirschie Johnson   +6 more
openaire   +3 more sources

Recent Advances in Photocatalyst‐Driven Protein Labeling and Proximity Mapping

The Chemical Record, EarlyView.
Photocatalyst‐driven protein labeling and proximity mapping have rapidly advanced as powerful strategies for spatiotemporal control in complex biological environments. This review integrates recent developments across single‐electron transfer and energy transfer‐based mechanisms and highlights how catalyst design, reactive intermediates, and diffusion ...
Shinichi Sato, Kazuki Miura, Juki Nakao, Hiroyuki Nakamura   +3 more
wiley   +1 more source

Protocol to quantify site-specific carbohydrate sulfation at immune checkpoint proteins in mammalian cells

STAR Protocols
Summary: Carbohydrate sulfotransferase 8 (CHST8) promotes immune checkpoint activation by sulfating GalNAcβ1-4GlcNAc (LacdiNAc) termini on glycoproteins.
Wen-Cheng Chou, Wei-Ting Chen, Yuh-Shan Jou, Chen-Yang Shen   +3 more
doaj   +1 more source

Diagnostic antibodies at an inflection point: From discovery platforms to design‐oriented engineering

VIEW, EarlyView.
This study systematically organizes the evolution of diagnostic antibodies from discovery platforms to design‐oriented engineering and analyzes the strengths and limitations of each approach. Meanwhile, it provides a critical, literature‐driven synthesis of current challenges, proposing integrated strategies for assay‐aware and function‐driven antibody
Qitao Song, Lulai Xu, Weilin Wu, Fei Xie, Yantong Pan, Tiantian Yang, Xiaoxia Xu, Yanyin Lin, Lihua Zhang, Jiahui Zhou, Junyou Zhou, Linping Li, Zhanhui Wang, Weijing Yi, Jing Wang   +14 more
wiley   +1 more source

A NanoLuc luciferase-based assay enabling the real-time analysis of protein secretion and injection by bacterial type III secretion systems.

, 2020
The elucidation of the molecular mechanisms of secretion through bacterial protein secretion systems is impeded by a shortage of assays to quantitatively assess secretion kinetics.
Bohn, Erwin, Grin, Iwan, Ursula Bilitewski, Sibel Westerhausen, Bilitewski, Ursula, Claudia E. Torres‐Vargas, Iwan Grin, Torres-Vargas, Claudia E, Wagner, Samuel, Erwin Bohn, Westerhausen, Sibel, Nowak, Melanie, Melanie Nowak, Samuel Wagner   +13 more
core   +1 more source

CRISPRa-mediated transcriptional activation of exogenous TdTomato and NanoLuc reporter constructs.

, 2022
(A) Schematic diagrams of the CRISPRa-VP64 and -p300 systems used. (B) Schematic diagrams of TdTomato and NanoLuc reporter gene constructs. The miniCMV-driven reporter construct has one gRNA binding site, whereas the TRE3G-driven reporter construct has ...
Jeffrey H. Miner (7126700), Kohei Omachi (4413616)   +1 more
core   +1 more source

Конструирование репортерных гриппозных онколитических векторов и оценка их безопасности при интракраниальном введении у крыс

Microbiology Independent Research Journal
ВВЕДЕНИЕ: Онколитические вирусы представляют собой перспективный инструмент для лечения злокачественных опухолей головного мозга в составе комплексной терапии.
А. А. Пулькина, А. С. Мустафаева, Е. А. Романовская-Романько, М. А. Плотникова, О. О. Ожерельева, М. А. Шуклина, Л. Н. Киселева, Ю. С. Алексеева, Н. С. Курмазов, А. Р. Муслимов, А.-П. С. Шурыгина, М. А. Стукова   +11 more
doaj   +1 more source