Results 71 to 80 of about 5,275 (178)

TOP1MT rs2293925 is an enhancer‐active regulatory SNP that shapes mitochondrial R‐loop dynamics

The FEBS Journal, EarlyView.
This study shows how a common genetic variant of mitochondrial topoisomerase 1 (TOP1MT rs2293925) can influence mitochondrial gene regulation, DNA topology, and formation of noncanonical nucleic acid structures such as R‐loops. By linking this enhancer‐active variant to mitochondrial nucleic acid stress in cellular contexts relevant to amyotrophic ...
Dóra Varga, Zsolt Ráduly, Beáta Boros‐Oláh, Éva Nagy, Zsolt Karányi, László Halász, Bálint László Bálint, György Vámosi, Emese Tóth, Péter Bai, Serena Santangelo, Patrizia Bossolasco, Antonia Ratti, Vincent Géli, Yves Pommier, Lóránt Székvölgyi   +15 more
wiley   +1 more source

Development of NanoLuc bridging immunoassay for detection of anti-drug antibodies

Journal of Immunological Methods, 2017
Anti-drug antibodies (ADAs) are generated in-vivo as an immune response to therapeutic antibody drugs and can significantly affect the efficacy and safety of the drugs. Hence, detection of ADAs is recommended by regulatory agencies during drug development process.
Nidhi Nath, Rod Flemming, Becky Godat, Marjeta Urh   +3 more
openaire   +2 more sources

A High-Throughput Inhibitor Screen Targeting CLAG3 Export and Membrane Insertion on Human Erythrocytes Infected with Malaria Parasites

Pathogens
To facilitate intracellular growth and replication, the virulent human malaria parasite P. falciparum remodels its host erythrocyte by exporting many proteins into the host cell cytosol.
Jinfeng Shao, Jonathan Chu, Kashif Mohammad, Sanjay A. Desai   +3 more
doaj   +1 more source

Lung Cancer Patients Display Increased Expression of Bovine Meat and Milk Factor (BMMF) Proteins in Peritumor Alveolar Macrophages

International Journal of Cancer, Volume 159, Issue 8, Page 2036-2053, 15 October 2026.
Plasmid‐like DNA sequences frequently found in bovine milk and serum have been proposed as infectious agents promoting indirect carcinogenesis. This multi‐omics study detected immune cells positive for these bovine meat and milk factors (BMMFs) in patients with lung cancer, with BMMF expression inversely correlated with smoking intensity.
Nives Cecere, Kristina Alikhanyan, Claudia Ernst, Lisa Häfele, Lars Feuerbach, Imke Grewe, Sumen Siqin, Karin Gunst, Gunjan Shukla, Veronika Frehtman, Claudia Tessmer, Florian Eichhorn, Michael Allgäuer, Alexander Brobeil, Ilse Hofmann, Barbara Leuchs, Marc A. Schneider, Timo Bund   +17 more
wiley   +1 more source

The chemokine receptor‐like fourth extracellular loop of the apelin receptor differentially regulates apelin and elabela binding and signalling

British Journal of Pharmacology, Volume 183, Issue 18, Page 5641-5656, September 2026.
Abstract Background and Purpose Apelin and Elabela are endogenous ligands of the apelin receptor (apelin receptor/APJ), a GPCR involved in cardiovascular regulation and body fluid homeostasis. The human receptor contains a conserved disulfide bridge linking the N‐terminal domain to extracellular loop 3 (ECL3) via Cys19 and Cys281, forming a structural ...
Chloé Bonef, Hela Bouhsine, Carine Huynh, Marion Chaigneau, Steven Dubois, Mikel Garcia‐Marcos, Catherine Llorens‐Cortes, Denis Servent, Philippe Robin, Hervé Nozach, Isabel Alves, Xavier Iturrioz   +11 more
wiley   +1 more source

Nonnodulating Fagales retain the functional NODULE INCEPTION gene

New Phytologist, Volume 251, Issue 6, Page 3080-3087, September 2026.
NODULE INCEPTION orthologs are present in nonnodulating species in Fagales.
Momona Noda, Momoyo Ito, Kana Miyata, Takuya Suzaki   +3 more
wiley   +1 more source

Transposase‐Assisted Donor Tethering Boosts Large‐Fragment HDR in Plants

Advanced Science, Volume 13, Issue 43, 3 August 2026.
A transposase‐assisted donor tethering strategy is developed to enhance homology‐directed repair in plants. By recruiting donor DNA to double‐strand breaks and synergizing with repair pathway reprogramming and transcription‐coupled donor design, this system markedly improves large‐fragment targeted insertion efficiency, providing a robust platform for ...
Sha Wei, Kai Zhang, Shuke Deng, Jinjie Chen, Xiaomei Huang, Jingfei Guo, Yuqing Wu, Yingjie Guo, Zhen Liang   +8 more
wiley   +1 more source

BODIPY-coelenterazine conjugates as self-illuminating substrates for NanoLuc

Chemical Communications
Excitation-free probes can exploit the spectroscopic properties of BODIPYs via bioluminescence resonance energy transference (BRET) triggered by luciferase-like enzymes.
Valeska Viereckt, Frank Abendroth, Alexander Schauerte, Marina Gerhard, Crispin Lichtenberg, Dmitri Kosenkov, Olalla Vázquez   +6 more
openaire   +2 more sources

Application of a novel HiBiT peptide tag for monitoring ATF4 protein expression in Neuro2a cells

Biochemistry and Biophysics Reports, 2017
A split NanoLuc assay system consisting of two fragments, large N-terminal and small C-terminal regions (NanoBiT), was developed to investigate protein-protein interactions within living cells.
Kentaro Oh-hashi, Eri Furuta, Keito Fujimura, Yoko Hirata   +3 more
doaj   +1 more source

Enhancing Optogenetics‐Based Cancer Therapy Via Nanotechnology

Exploration, Volume 6, Issue 4, August 2026.
Nanotechnology enhances optogenetics‐based cancer therapy through improved delivery systems and non‐invasive optical regulation. It further enables precise and programmable control of optogenetic living therapeutics for localized antitumor responses.
Honggang Shen, Wenzhe Yi, Rongzhang Li, Shuangshuang Hu, Xindi Qian, Dan Yan, Qian Zou, Guanru Wang, Yaping Li   +8 more
wiley   +1 more source