Results 91 to 100 of about 5,929 (191)

BODIPY-coelenterazine conjugates as self-illuminating substrates for NanoLuc

Chemical Communications
Excitation-free probes can exploit the spectroscopic properties of BODIPYs via bioluminescence resonance energy transference (BRET) triggered by luciferase-like enzymes.
Valeska Viereckt, Frank Abendroth, Alexander Schauerte, Marina Gerhard, Crispin Lichtenberg, Dmitri Kosenkov, Olalla Vázquez   +6 more
openaire   +2 more sources

Expanding the bioluminescence reporter toolkit for plant chronobiology with NanoLUC [PDF]

, 2018
Abstract Bioluminescence has been an important tool for gathering circadian data with the main reporter gene exploited firefly luciferase (LUC). In some circumstances the rapid inactivation of LUC could be disadvantageous, e.g. reporting total protein levels through reporter translational fusions. In the latter scenario the commercially
Urquiza-García, Uriel, Millar, Andrew J.   +1 more
openaire   +1 more source

Application of a novel HiBiT peptide tag for monitoring ATF4 protein expression in Neuro2a cells

Biochemistry and Biophysics Reports, 2017
A split NanoLuc assay system consisting of two fragments, large N-terminal and small C-terminal regions (NanoBiT), was developed to investigate protein-protein interactions within living cells.
Kentaro Oh-hashi, Eri Furuta, Keito Fujimura, Yoko Hirata   +3 more
doaj   +1 more source

Akaluc bioluminescence offers superior sensitivity to track in vivo dynamics of SARS-CoV-2 infection

iScience
Summary: Monitoring in vivo viral dynamics can improve our understanding of pathogenicity and tissue tropism. Because the gene size of RNA viruses is typically small, NanoLuc is the primary choice for accommodation within viral genome.
Tomokazu Tamura, Hayato Ito, Shiho Torii, Lei Wang, Rigel Suzuki, Shuhei Tsujino, Akifumi Kamiyama, Yoshitaka Oda, Masumi Tsuda, Yuhei Morioka, Saori Suzuki, Kotaro Shirakawa, Kei Sato, Kumiko Yoshimatsu, Yoshiharu Matsuura, Satoshi Iwano, Shinya Tanaka, Takasuke Fukuhara   +17 more
doaj   +1 more source

Bioluminescence Profiling of NanoKAZ/NanoLuc Luciferase Using a Chemical Library of Coelenterazine Analogues

, 2020
International audienceWe describe here an extensive structure-bioluminescence relationship study of a chemical library of analogues of coelenterazine, using nanoKAZ/NanoLuc, a mutated luciferase originated from the catalytic subunit of the deep-sea ...
Jacob, Yves, Janin, Yves, Louis, Baatallah, Racha, Goyard, Sophie, Coutant, Eloi, P, Hervin, Vincent, Rose, Thierry, Gagnot, Glwadys   +7 more
core   +1 more source

Nanoluc oligoproteins as a model system for protein misfolding and refolding studies

Biophysical Journal
Protein misfolding can lead to protein malfunction, which may compromise cell viability. Chaperones, including the HSP70 system, are proteins that have evolved to restore the native structure of misfolded proteins. Although most chaperones, including DnaK (bacterial HSP70), were first described over 30 years ago, important questions related to their ...
Grigory V. Mechetin, Piotr E. Marszalek
openaire   +2 more sources

Recovery attempts of recCA09 with engineered combinations of independently emerged resistance mutations (genetic background: recCA09-nanoLuc).

Recovery attempts of recCA09 with engineered combinations of independently emerged resistance mutations (genetic background: recCA09-nanoLuc).
Jeong-Joong Yoon (221027), Richard K. Plemper (221041), Michael G. Natchus (1884760), Robert M. Cox (8029568), Megha Aggarwal (293831), Nicole A. Lieberman (17889630), Alexander L. Greninger (8677377), Elizabeth B. Sobolik (17889633), Carolin M. Lieber (15307134), Hae-Ji Kang (6889463)   +9 more
core   +1 more source

Anticuerpos monodominio fusionados a la luciferasa NanoLuc para el diagnóstico de enfermedades infecciosas y alergias [PDF]

En esta tesis se exploró el potencial de generar proteínas quiméricas, formadas por nanobodies unidos a la enzima NanoLuc, para su uso como reactivos de detección en inmunoensayos.
Segovia de los Santos, Paula
core   +1 more source

Abstract LB-137: Developing preclinical mouse models that employ fluorophore-nanoLuc BRET reporters (LumiFluor) for monitoring tumorigenesis and response to therapy

, 2017
Fluorescent proteins are widely used to study molecular and cellular events, yet this traditionally relies on delivery of excitation light, which can trigger autofluorescence, photoxicity, and photobleaching, impairing their use in vivo.
Scott Troutman, Miranda Carper, John Cleveland, Erin Henry, Joseph Kissil, Franz Schaub, Weimin Li, Adele Musicant, Antonio L. Amelio   +8 more
core   +1 more source

EC₅₀ values and assay parameters following treatment with Amphotericin B and Miltefosine against wild-type and NanoLuc-PEST expressing L. mexicana (see S5 Fig for full dataset).

, 2018
EC50 values and assay parameters following treatment with Amphotericin B and Miltefosine against wild-type and NanoLuc-PEST expressing L. mexicana (see S5 Fig for full dataset).
Hamza Hameed (5508482), Sarah L. Berry (5508476), Somaia Saif Abou-Akkada (5508485), Marissa L. Maciej-Hulme (5508479), Paul Horrocks (49038), Anna Thomason (3558581), Helen P. Price (183336)   +6 more
core   +1 more source