Results 91 to 100 of about 6,439 (202)

BODIPY-coelenterazine conjugates as self-illuminating substrates for NanoLuc

Chemical Communications
Excitation-free probes can exploit the spectroscopic properties of BODIPYs via bioluminescence resonance energy transference (BRET) triggered by luciferase-like enzymes.
Valeska Viereckt, Frank Abendroth, Alexander Schauerte, Marina Gerhard, Crispin Lichtenberg, Dmitri Kosenkov, Olalla Vázquez   +6 more
openaire   +2 more sources

Expanding the bioluminescence reporter toolkit for plant chronobiology with NanoLUC [PDF]

, 2018
Abstract Bioluminescence has been an important tool for gathering circadian data with the main reporter gene exploited firefly luciferase (LUC). In some circumstances the rapid inactivation of LUC could be disadvantageous, e.g. reporting total protein levels through reporter translational fusions. In the latter scenario the commercially
Urquiza-García, Uriel, Millar, Andrew J.   +1 more
openaire   +1 more source

Natural Killer Cell‐Derived Extracellular Vesicle Preparations Demonstrate Potent Antitumor Activity Against Human Glioblastoma In Vitro and In Vivo

Journal of Extracellular Biology, Volume 5, Issue 10, October 2026.
Schematic overview of NK‐EV isolation strategies from expanded primary human NK cells. Primary human NK cells were isolated from PBMCs and expanded using K562 feeder cells. EV‐containing preparations were then isolated from expanded NK‐cell cultures using two approaches. First, total exosome isolation reagent was used as a precipitation‐based method to
Huy Phuoc Quang Nguyen, Meesun Yoon, Shin Jung, Duck Cho, Byong Seung Cho, Tung Nguyen Thanh Uong, Jae‐Uk Jeong, Yong‐Hyub Kim, Nhat Phuoc Nguong Minh Nguyen, Tien Luyen Vu   +9 more
wiley   +1 more source

Expression and functional analysis of the NanoLuc Luciferase reporter in plants

, 2022
Reporter genes serve as important tools in cellular and molecular biology research. Among the commonly used reporters, bioluminescence-based reporters such as the firefly luciferase (FLuc) represent a versatile subclass which allows both imaging- and ...
Singh, Atinder
core  

Application of a novel HiBiT peptide tag for monitoring ATF4 protein expression in Neuro2a cells

Biochemistry and Biophysics Reports, 2017
A split NanoLuc assay system consisting of two fragments, large N-terminal and small C-terminal regions (NanoBiT), was developed to investigate protein-protein interactions within living cells.
Kentaro Oh-hashi, Eri Furuta, Keito Fujimura, Yoko Hirata   +3 more
doaj   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, Volume 600, Issue 18, Page 2777-2786, September 2026.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Versatile Detection of Cellular Protein via Fluorescence Anisotropy

Advanced Science, Volume 13, Issue 51, 14 September 2026.
Cell Lysate Fluorescence Anisotropy (CFAST) utilizes long lifetime dye‐labeled nanobodies for rapid protein quantification in minimally purified, complex cell lysate. When coupled with cellular thermal shift, CFAST allows high‐throughput detection of endogenous protein‐small molecule engagement, facilitating the discovery of novel binders for ...
Qing Tang, Yuan‐Ping Wei, Ricky Ruiqi Ma, Na Wei, Tammy Zihan Zhou, Hilary Kung‐Yu Ho, Zafar Iqbal Bhat, Iqra Ishrat, Xiaoyu Li, Judy Wai Ping Yam, Yung Hou Wong, Qiankun Wang, Justin L. Tan   +12 more
wiley   +1 more source

Development of a Ready‐To‐Use Bioluminescence Immunosensor for the One‐Step Sensitive Detection of Antibodies Against African Swine Fever Virus

Microbial Biotechnology
Rapid and reliable analytical techniques play important roles in various research fields and are particularly crucial for diagnosing infectious diseases in clinical settings.
Zhonghui Zhang, Xuesai Li, Qingli Niu, Jinming Wang, Yanghe Liu, Dossêh Jean Apôtre Afayibo, Wenting Chen, Songlin Yang, Hong Yin, Guiquan Guan, Jifei Yang   +10 more
doaj   +1 more source

Inhibition of KDEL Receptors Remodels the Tumor Microenvironment for T Cell Independent Tumor Regression

Advanced Science, Volume 13, Issue 51, 14 September 2026.
Inhibition of KDELR2 in a small fraction of tumor cells generates sustainable immunogenic cell death conditions within the tumor microenvironment. These conditions promote the regression of tumors in a T cell independent manner. During regression, macrophages prime T cells that subsequently provide systemic protection against recurrence. The potency of
Shakti P Pattanayak, Hong Wang, Belinda Willard, Timothy A. Chan, William C Merrick, Zheng‐Rong Lu, Boaz Tirosh   +6 more
wiley   +1 more source

Programmable Nanobody‐Targeting Chimeras Enable Intracellular Viral Protein Degradation

Advanced Science, Volume 13, Issue 53, 24 September 2026.
Nab‐TAC enables targeted degradation of HBV surface antigen (HBsAg) in vivo. By fusing nanobody‐based recognition domains with programmable proteasome‐recruiting degradation signals, Nab‐TAC reduces HBsAg levels in a hydrodynamic HBV mouse model. ABSTRACT Chronic hepatitis B virus (HBV) infection remains a major global health challenge, largely because
Max Yu‐Chen Pan, Ting‐Hui Lee, Tzu‐Ning Peng, Eva Yi‐Hsuan Wu, Yu‐Ting Chiu, Chih‐Chien Chiu, Hong Vinh Nguyen, Yuan‐Shao Pao, Ming‐Hong Chao, Richard Kuan‐Lin Lee, Han‐Chung Wu, Lily Hui‐Ching Wang   +11 more
wiley   +1 more source