Results 81 to 90 of about 5,275 (178)

Expanding the bioluminescence reporter toolkit for plant chronobiology with NanoLUC [PDF]

, 2018
Abstract Bioluminescence has been an important tool for gathering circadian data with the main reporter gene exploited firefly luciferase (LUC). In some circumstances the rapid inactivation of LUC could be disadvantageous, e.g. reporting total protein levels through reporter translational fusions. In the latter scenario the commercially
Urquiza-García, Uriel, Millar, Andrew J.   +1 more
openaire   +1 more source

Autophagy Plays a Suppressive Role in Bladder Tumor Formation in an Orthotopic Mouse Model and Bladder Cancer Patient Specimens

The Kaohsiung Journal of Medical Sciences, Volume 42, Issue 8, August 2026.
ABSTRACT Autophagy plays either a suppressing or promoting role during tumor development. Clarifying the role of autophagy in bladder tumorigenesis both in vitro and in vivo is crucial for developing novel therapeutic strategies through manipulating autophagy activity.
Wan‐Ting Kuo, Chin‐Chen Pan, Yi‐Wen Liu, Nan‐Haw Chow, Hong‐Lin Cheng, Shan‐Ying Wu, Sheng‐Hui Lan, Chih‐Peng Chang, Hsiao‐Sheng Liu   +8 more
wiley   +1 more source

Recent Advances in Photocatalyst‐Driven Protein Labeling and Proximity Mapping

The Chemical Record, Volume 26, Issue 8, August 2026.
Photocatalyst‐driven protein labeling and proximity mapping have rapidly advanced as powerful strategies for spatiotemporal control in complex biological environments. This review integrates recent developments across single‐electron transfer and energy transfer‐based mechanisms and highlights how catalyst design, reactive intermediates, and diffusion ...
Shinichi Sato, Kazuki Miura, Juki Nakao, Hiroyuki Nakamura   +3 more
wiley   +1 more source

A Dual-Purpose Real-Time Indicator and Transcriptional Integrator for Calcium Detection in Living Cells. [PDF]

ACS Synth Biol, 2022
Erdenee E, Ting AY.
europepmc   +1 more source

Exploring the BICC1 Interactome in HEK293T Cells: Insights Into RNA‐Mediated Protein Networks and Biomolecular Condensates

FASEB BioAdvances, Volume 8, Issue 8, August 2026.
Workflow for BICC1 interactome characterization in HEK293T cells. Cells expressing FLAG‐tagged BICC1 (full‐length or ΔSAM) were subjected to co‐immunoprecipitation under RNA‐preserved (−) or RNase‐treated (+) conditions (1). Interacting proteins were identified by LC–MS/MS (2) and analyzed through protein–protein interaction networks and functional ...
Heloísa Monteiro do Amaral‐Prado, Gabriela Alves Moreira, Cristiane Santos, Fernando Henrique Bosso, Felipe Eduardo Ciamponi, Guilherme Oliveira Barbosa, Mario Henrique Bengtson, Katlin Brauer Massirer   +7 more
wiley   +1 more source

Anticodon-edited tRNA enables translational readthrough of COL4A5 premature termination codons.

PLoS ONE
Alport syndrome is caused by variants in COL4A3, COL4A4, or COL4A5, which encode the α3α4α5 chains of type IV collagen. These variants result in defects in the glomerular basement membrane (GBM) and impaired kidney function.
Kohei Omachi, Joseph J Porter, John D Lueck, Jeffrey H Miner   +3 more
doaj   +1 more source

Akaluc bioluminescence offers superior sensitivity to track in vivo dynamics of SARS-CoV-2 infection

iScience
Summary: Monitoring in vivo viral dynamics can improve our understanding of pathogenicity and tissue tropism. Because the gene size of RNA viruses is typically small, NanoLuc is the primary choice for accommodation within viral genome.
Tomokazu Tamura, Hayato Ito, Shiho Torii, Lei Wang, Rigel Suzuki, Shuhei Tsujino, Akifumi Kamiyama, Yoshitaka Oda, Masumi Tsuda, Yuhei Morioka, Saori Suzuki, Kotaro Shirakawa, Kei Sato, Kumiko Yoshimatsu, Yoshiharu Matsuura, Satoshi Iwano, Shinya Tanaka, Takasuke Fukuhara   +17 more
doaj   +1 more source

Nanoluc oligoproteins as a model system for protein misfolding and refolding studies

Biophysical Journal
Protein misfolding can lead to protein malfunction, which may compromise cell viability. Chaperones, including the HSP70 system, are proteins that have evolved to restore the native structure of misfolded proteins. Although most chaperones, including DnaK (bacterial HSP70), were first described over 30 years ago, important questions related to their ...
Grigory V. Mechetin, Piotr E. Marszalek
openaire   +2 more sources

Abstracts

Molecular Oncology, Volume 20, Issue S1, Page 1-692, August 2026.
Abstracts submitted to the ‘EACR 2026 Congress: Innovative Cancer Science’, from 08–11 June 2026 and accepted by the Congress Organising Committee are published in this Supplement of Molecular Oncology, an affiliated journal of the European Association for Cancer Research (EACR).
wiley   +1 more source

The long-lasting expression of recombinant artLCMV following intraperitoneal administration exerts potent antitumor effects on tumor models of peritoneal carcinomatosis

OncoImmunology
Peritoneal carcinomatosis remains a challenging clinical condition with limited therapeutic options. In this study, we evaluated the efficacy of a recombinant artLCMV platform encoding tumor antigens and immune-stimulatory molecules in preclinical models.
Celia Gomar, Aline Risson, Leire Arrizabalaga, Nuria Ardaiz, Virginia Belsue, Adrian Gil-Korilis, Sarah Ahmadi-Erber, Timo Schippers, Ignacio Melero, Klaus K. Orlinger, Fernando Aranda, Henning Lauterbach, Pedro Berraondo   +12 more
doaj   +1 more source