Results 81 to 90 of about 6,439 (202)

Protocol to quantify site-specific carbohydrate sulfation at immune checkpoint proteins in mammalian cells

STAR Protocols
Summary: Carbohydrate sulfotransferase 8 (CHST8) promotes immune checkpoint activation by sulfating GalNAcβ1-4GlcNAc (LacdiNAc) termini on glycoproteins.
Wen-Cheng Chou, Wei-Ting Chen, Yuh-Shan Jou, Chen-Yang Shen   +3 more
doaj   +1 more source

Human APOBEC3G suppresses homologous recombination and LIG4‐independent end joining in DNA double‐strand break repair

The FEBS Journal, EarlyView.
Chromosomal DNA double‐strand breaks (DSBs) are repaired by homologous recombination and nonhomologous end joining (NHEJ). Recent work has additionally established theta‐mediated end‐joining (TMEJ) as a mechanism for DSB joining. Cells lacking NHEJ and TMEJ can repair DSBs in a homology‐dependent manner.
Shinta Saito, Miori Murayama, Usaki Arai, Sekifumi Akatsu, Ami Yamamoto, Misaki Kadoguchi, Noritaka Adachi   +6 more
wiley   +1 more source

TOP1MT rs2293925 is an enhancer‐active regulatory SNP that shapes mitochondrial R‐loop dynamics

The FEBS Journal, EarlyView.
This study shows how a common genetic variant of mitochondrial topoisomerase 1 (TOP1MT rs2293925) can influence mitochondrial gene regulation, DNA topology, and formation of noncanonical nucleic acid structures such as R‐loops. By linking this enhancer‐active variant to mitochondrial nucleic acid stress in cellular contexts relevant to amyotrophic ...
Dóra Varga, Zsolt Ráduly, Beáta Boros‐Oláh, Éva Nagy, Zsolt Karányi, László Halász, Bálint László Bálint, György Vámosi, Emese Tóth, Péter Bai, Serena Santangelo, Patrizia Bossolasco, Antonia Ratti, Vincent Géli, Yves Pommier, Lóránt Székvölgyi   +15 more
wiley   +1 more source

A comprehensive study on ferredoxin isoforms in the cyanobacterium Synechocystis sp. PCC 6803

The FEBS Journal, EarlyView.
Ferredoxins are small FeS cluster‐containing proteins that play a central role in distributing electrons within the cell. The cyanobacterium Synechocystis sp. PCC 6803 contains several ferredoxin isoforms and their biophysical properties (UV/Vis and EPR spectra, Em potential), expression patterns, and interactions with the electron donors photosystem I
Marko Boehm, Drazenka Svedruzic, Carolyn E. Lubner, Jens Appel, David W. Mulder, Effie C. Kisgeropoulos, Vanessa Hueren, Katharina Spengler, Vivek S. Bharadwaj, Zhanjun Guo, Anastasia E. Ledinina, Darja Deobald, Lorenz Adrian, Paul W. King, Kirstin Gutekunst   +14 more
wiley   +1 more source

Determining the EC50 of Tracer-6908 with ACVR1/ALK2-NanoLuc

, 2018
Promega had provided us with Tracer-6908 that was developed collaboratively with SGC. Before the tracer can be used in nanoBRET assay for compound IC50, it is necessary to determine the optimal tracer concentration.
Wong, Jong Fu, Wong, Jong Fu (5163044), Bullock, Alex, Bullock, Alex (4954282)   +3 more
core   +2 more sources

Far‐field bioluminescent immune cell–mediated photodynamic therapy is limited by photon flux

Photochemistry and Photobiology, EarlyView.
Bioluminescent immune cell‐mediated photodynamic therapy uses tumor‐homing, luciferase‐expressing immune cells as internal light sources to activate photosensitizers in deep‐seated and/or micrometastatic tumors. Using both experiments and computational simulations, we find that this approach delivers insufficient far‐field energy to tumor cells to ...
Ji Tae Park, Sudip Timilsina, Rebecca C. Harman, Chi‐Yong Eom, Jeonghyun Oh, Qianqian Fang, Nozomi Nishimura, Chris B. Schaffer, Bryan Q. Spring   +8 more
wiley   +1 more source

A High-Throughput Inhibitor Screen Targeting CLAG3 Export and Membrane Insertion on Human Erythrocytes Infected with Malaria Parasites

Pathogens
To facilitate intracellular growth and replication, the virulent human malaria parasite P. falciparum remodels its host erythrocyte by exporting many proteins into the host cell cytosol.
Jinfeng Shao, Jonathan Chu, Kashif Mohammad, Sanjay A. Desai   +3 more
doaj   +1 more source

Lung Cancer Patients Display Increased Expression of Bovine Meat and Milk Factor (BMMF) Proteins in Peritumor Alveolar Macrophages

International Journal of Cancer, Volume 159, Issue 8, Page 2036-2053, 15 October 2026.
Plasmid‐like DNA sequences frequently found in bovine milk and serum have been proposed as infectious agents promoting indirect carcinogenesis. This multi‐omics study detected immune cells positive for these bovine meat and milk factors (BMMFs) in patients with lung cancer, with BMMF expression inversely correlated with smoking intensity.
Nives Cecere, Kristina Alikhanyan, Claudia Ernst, Lisa Häfele, Lars Feuerbach, Imke Grewe, Sumen Siqin, Karin Gunst, Gunjan Shukla, Veronika Frehtman, Claudia Tessmer, Florian Eichhorn, Michael Allgäuer, Alexander Brobeil, Ilse Hofmann, Barbara Leuchs, Marc A. Schneider, Timo Bund   +17 more
wiley   +1 more source

Identifying optimal capsid duplication length for the stability of reporter flaviviruses

Emerging Microbes and Infections, 2020
Mosquito-transmitted flaviviruses cause widespread disease across the world. To provide better molecular tools for drug screens and pathogenesis studies, we report a new approach to produce stable NanoLuc-tagged flaviviruses, including dengue virus ...
Coleman Baker, Yang Liu, Jing Zou, Antonio Muruato, Xuping Xie, Pei-Yong Shi   +5 more
doaj   +1 more source

Helicobacter pylori Outer Membrane Vesicles Engage TLR2 Through NapA to Promote Epithelial Association and Inflammatory Signalling

Journal of Extracellular Vesicles, Volume 15, Issue 10, October 2026.
ABSTRACT Helicobacter pylori infection is characterized by chronic gastric inflammation driven by persistent activation of innate immune signalling pathways. Outer membrane vesicles (OMVs) released by H. pylori are important mediators of host–pathogen interactions. Although several mechanisms of OMV internalization have been described, how specific OMV‐
Mariaignacia Rubilar, Rosy Brito, Annegrett Palavecino, Joan Villena, Marco Mellado, María I. Silva, Manuel Valenzuela‐Valderrama   +6 more
wiley   +1 more source