Results 81 to 90 of about 5,929 (191)

Identifying optimal capsid duplication length for the stability of reporter flaviviruses

Emerging Microbes and Infections, 2020
Mosquito-transmitted flaviviruses cause widespread disease across the world. To provide better molecular tools for drug screens and pathogenesis studies, we report a new approach to produce stable NanoLuc-tagged flaviviruses, including dengue virus ...
Coleman Baker, Yang Liu, Jing Zou, Antonio Muruato, Xuping Xie, Pei-Yong Shi   +5 more
doaj   +1 more source

Activation of peripheral NOP receptors reduces periorbital mechanical allodynia evoked by CGRP in mice

British Journal of Pharmacology, EarlyView.
Background and Purpose Migraine is a neurovascular disorder largely mediated by calcitonin gene‐related peptide (CGRP). This study explores the role of the nociceptin/orphanin FQ (N/OFQ)–N/OFQ receptor (NOP) system in CGRP‐induced periorbital mechanical allodynia (PMA) in mice.
Chiara Sturaro, Pietro Pola, Michela Argentieri, Alessia Frezza, Matilde Marini, Francesco De Logu, Valentina Albanese, Marie Soukupova, Davide Malfacini, Nurulain T. Zaveri, Romina Nassini, Dane D. Jensen, Pierangelo Geppetti, Girolamo Calò, Chiara Ruzza   +14 more
wiley   +1 more source

Targeting KRAS for cancer therapy

British Journal of Pharmacology, EarlyView.
In recent years, therapeutics targeted against KRAS proto‐oncogene GTPase (KRAS)‐mutant cancers have seen significant progress. Herein we outline the biology and epidemiology of KRAS alterations at the lineage and allele levels, reviewing the clinical evidence for KRASG12C inhibition from the discovery of the recessive switch pocket to sotorasib ...
Jianlong Jia, Ruonan Liu, Tonia A. Adamide, Isis E. Fernandez, Georgios T. Stathopoulos   +4 more
wiley   +1 more source

Expression and functional analysis of the NanoLuc Luciferase reporter in plants

, 2022
Reporter genes serve as important tools in cellular and molecular biology research. Among the commonly used reporters, bioluminescence-based reporters such as the firefly luciferase (FLuc) represent a versatile subclass which allows both imaging- and ...
Singh, Atinder
core  

Nonnodulating Fagales retain the functional NODULE INCEPTION gene

New Phytologist, EarlyView.
NODULE INCEPTION orthologs are present in nonnodulating species in Fagales.
Momona Noda, Momoyo Ito, Kana Miyata, Takuya Suzaki   +3 more
wiley   +1 more source

Development of NanoLuc bridging immunoassay for detection of anti-drug antibodies

Journal of Immunological Methods, 2017
Anti-drug antibodies (ADAs) are generated in-vivo as an immune response to therapeutic antibody drugs and can significantly affect the efficacy and safety of the drugs. Hence, detection of ADAs is recommended by regulatory agencies during drug development process.
Nidhi Nath, Rod Flemming, Becky Godat, Marjeta Urh   +3 more
openaire   +2 more sources

Fluorophore-NanoLuc BRET Reporters Enable Sensitive In Vivo Optical Imaging and Flow Cytometry for Monitoring Tumorigenesis

, 2015
Fluorescent proteins are widely used to study molecular and cellular events, yet this traditionally relies on delivery of excitation light, which can trigger autofluorescence, photoxicity, and photobleaching, impairing their use in vivo.
Reza, Md Shamim, Cleveland, John L., Hoxha, Sany, Musicant, Adele M., Li, Weimin, Amelio, Antonio L., Schaub, Franz X., Flaveny, Colin A., Guo, Min   +8 more
core   +1 more source

A High-Throughput Inhibitor Screen Targeting CLAG3 Export and Membrane Insertion on Human Erythrocytes Infected with Malaria Parasites

Pathogens
To facilitate intracellular growth and replication, the virulent human malaria parasite P. falciparum remodels its host erythrocyte by exporting many proteins into the host cell cytosol.
Jinfeng Shao, Jonathan Chu, Kashif Mohammad, Sanjay A. Desai   +3 more
doaj   +1 more source

Glucocorticoid Receptor Translational Isoforms Generate Unique Glucocorticoid Responses in the Mouse Brain

The FASEB Journal, Volume 40, Issue 12, 30 June 2026.
GR‐A knockin mice were generated that express the classic full‐length glucocorticoid receptor (GR‐A) but lack the highly conserved translational isoforms GR‐B, GR‐C1, GR‐C2, GR‐C3, GR‐D1, GR‐D2, and GR‐D3 that have progressively shorter N‐terminal transactivation domains (NTDs).
Robert H. Oakley, Tatsuya Sueyoshi, Sivapriya Ramamoorthy, Viktoriya D. Nikolova, David R. Goulding, Jianying Li, Manas K. Ray, Sheryl S. Moy, John A. Cidlowski   +8 more
wiley   +1 more source

Revisiting Hydrazine Moiety as a Scaffold for the Synthesis of Potential Antiviral Pyrazole Ribonucleoside Analogues

ChemMedChem, Volume 21, Issue 11, 15 June 2026.
Three series of pyrazole ribonucleoside analogues were designed and synthesized. They were evaluated, along with some of their pronucleotides, for their antiviral activity against RNA viruses. Pyrazole ribonucleoside analogues were prepared via a linear approach starting with the reaction of D‐ribosylhydrazine with 1,3‐dicarbonyl or acrylonitrile ...
Anaïs Guillou, Nathalie Gros, Delphine Muriaux, Suzanne Peyrottes, Jean‐Jacques Vasseur, Michael Smietana, Christophe Mathé   +6 more
wiley   +1 more source