Construction of a Dengue NanoLuc Reporter Virus for In Vivo Live Imaging in Mice [PDF]
Viruses, 2022 Since the first isolation in 1943, the dengue virus (DENV) has spread throughout the world, but effective antiviral drugs or vaccines are still not available. To provide a more stable reporter DENV for vaccine development and antiviral drug screening, we
Enyue Fang +10 more
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Visualization of yellow fever virus infection in mice using a bioluminescent reporter virus [PDF]
Emerging Microbes and Infections, 2021 Yellow fever virus (YFV) is a re-emerging flavivirus, which can lead to severe clinical manifestations and high mortality, with no specific antiviral therapies available. The live-attenuated yellow fever vaccine 17D (YF17D) has been widely used for over eighty years.
, Zhong-Yu Liu, Cheng-Feng Qin
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Generation and Characterization of an Influenza D Reporter Virus. [PDF]
Viruses, 2023 Influenza D virus (IDV) can infect various livestock animals, such as cattle, swine, and small ruminants, and was shown to have zoonotic potential. Therefore, it is important to identify viral factors involved in the broad host tropism and identify potential antiviral compounds that can inhibit IDV infection.
Probst L +7 more
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Rapid Construction and Characterization of Infectious cDNA Clones and Reporter Viruses of Enteroviruses, Including Enterovirus A71 and Coxsackievirus B5, with Systematic Identification of Critical Determinants for Successful Reporter Virus Generation [PDF]
VirusesEnteroviruses are positive-sense single-stranded RNA viruses and common pathogens that are responsible for diverse public health diseases. To facilitate the study of the virus biology and pathogenesis of enterovirus, we developed a rapid method for ...
Hao Zheng +6 more
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Influenza virus assays based on virus‐inducible reporter cell lines [PDF]
Influenza and Other Respiratory Viruses, 2009 Background Virus‐inducible reporter genes have been used as the basis of virus detection and quantitation assays for a number of viruses. A strategy for influenza A virus‐induction of a reporter gene was recently described. In this report, we describe the extension of this strategy to influenza B virus, the generation of stable cell lines with ...
Andrew Pekosz
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Development of Virus-Like-Particle Vaccine and Reporter Assay for Zika Virus [PDF]
Journal of Virology, 2017 ABSTRACT Recent worldwide outbreaks of Zika virus (ZIKV) infection and the lack of an approved vaccine raise serious concerns regarding preparedness to combat this emerging virus. We used a virus-like particle (VLP)-based approach to develop a vaccine and a microneutralization assay for ZIKV.
Himanshu Garg, Anjali Joshi
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Multi-color fluorescent reporter dengue viruses with improved stability for analysis of a multi-virus infection. [PDF]
PLoS ONE, 2018 Reporter virus is a versatile tool to visualize and to analyze virus infections. However, for flaviviruses, it is difficult to maintain the inserted reporter genes on the viral genome, limiting its use in several studies that require homogeneous virus ...
Amporn Suphatrakul +6 more
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Use of Reporter Genes in the Generation of Vaccinia Virus-Derived Vectors
Viruses, 2016 Vaccinia virus (VACV) is one of the most extensively-studied viruses of the Poxviridae family. It is easy to genetically modify, so it has become a key tool for many applications.
Sally Al Ali +3 more
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Labeling Ebola Virus with a Self-Splicing Fluorescent Reporter
Microorganisms, 2022 Inteins (intervening proteins) are polypeptides that interrupt the sequence of other proteins and remove themselves through protein splicing. In this intein-catalyzed reaction, the two peptide bonds surrounding the intein are rearranged to release the ...
Baylee Heiden +3 more
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Generation of a HiBiT-expressing recombinant rat hepacivirus supporting both in vivo and in vitro infection. [PDF]
PLoS PathogensThe lack of immuno-competent animal models of hepatitis C virus (HCV) infection has been an obstacle to vaccine development and research on immune responses.
Yasunori Akaike +5 more
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