Results 11 to 20 of about 174,549 (256)

Generation and Characterization of an Influenza D Reporter Virus. [PDF]

Viruses, 2023
Influenza D virus (IDV) can infect various livestock animals, such as cattle, swine, and small ruminants, and was shown to have zoonotic potential. Therefore, it is important to identify viral factors involved in the broad host tropism and identify potential antiviral compounds that can inhibit IDV infection.
Probst L, Laloli L, Licheri MF, Licheri M, Gultom M, Holwerda M, V'kovski P, Dijkman R.   +7 more
europepmc   +4 more sources

Subgenomic reporter RNA system for detection of alphavirus infection in mosquitoes. [PDF]

PLoS ONE, 2013
Current methods for detecting real-time alphavirus (Family Togaviridae) infection in mosquitoes require the use of recombinant viruses engineered to express a visibly detectable reporter protein.
J Jordan Steel, Alexander W E Franz, Irma Sanchez-Vargas, Ken E Olson, Brian J Geiss   +4 more
doaj   +1 more source

Construction and Generation of a Recombinant Senecavirus a Stably Expressing the NanoLuc Luciferase for Quantitative Antiviral Assay

Frontiers in Microbiology, 2021
Senecavirus A (SVA), also known as Seneca Valley virus, is a recently emerged picornavirus that can cause swine vesicular disease, posing a great threat to the global swine industry.
Xiaoran Guo, Kuan Zhao, Xiaona Liu, Baishi Lei, Wuchao Zhang, Xiuli Li, Wanzhe Yuan, Wanzhe Yuan, Wanzhe Yuan   +8 more
doaj   +1 more source

Identifying optimal capsid duplication length for the stability of reporter flaviviruses

Emerging Microbes and Infections, 2020
Mosquito-transmitted flaviviruses cause widespread disease across the world. To provide better molecular tools for drug screens and pathogenesis studies, we report a new approach to produce stable NanoLuc-tagged flaviviruses, including dengue virus ...
Coleman Baker, Yang Liu, Jing Zou, Antonio Muruato, Xuping Xie, Pei-Yong Shi   +5 more
doaj   +1 more source

Fitness of mCherry Reporter Tick-Borne Encephalitis Virus in Tick Experimental Models

Viruses, 2022
The tick-borne encephalitis virus (TBEV) causes a most important viral life-threatening illness transmitted by ticks. The interactions between the virus and ticks are largely unexplored, indicating a lack of experimental tools and systematic studies. One
Ádám Kevély, Veronika Prančlová, Monika Sláviková, Jan Haviernik, Václav Hönig, Eva Nováková, Martin Palus, Daniel Růžek, Boris Klempa, Juraj Koči   +9 more
doaj   +1 more source

Using recombination-dependent lethal mutations to stabilize reporter flaviviruses for rapid serodiagnosis and drug discovery

EBioMedicine, 2020
Background: Many flaviviruses are significant human pathogens that cause global public health threats. Developing research tools for studying and diagnosing these pathogens is a top priority.
Coleman Baker, Xuping Xie, Jing Zou, Antonio Muruato, Katja Fink, Pei-Yong Shi   +5 more
doaj   +1 more source

Dual-fluorescent reporter for live-cell imaging of the ER during DENV infection

Frontiers in Cellular and Infection Microbiology, 2022
Infection by flaviviruses leads to dramatic remodeling of the endoplasmic reticulum (ER). Viral replication occurs within virus-induced vesicular invaginations in the ER membrane.
Lochlain Corliss, Madeline Holliday, Nicholas J. Lennemann   +2 more
doaj   +1 more source

Fluorescent and Bioluminescent Reporter Myxoviruses

Viruses, 2016
The advent of virus reverse genetics has enabled the incorporation of genetically encoded reporter proteins into replication-competent viruses. These reporters include fluorescent proteins which have intrinsic chromophores that absorb light and re-emit ...
Christina A. Rostad, Michael C. Currier, Martin L. Moore   +2 more
doaj   +1 more source

Construction, Characterization and Application of Recombinant Porcine Deltacoronavirus Expressing Nanoluciferase

Viruses, 2021
Porcine deltacoronavirus (PDCoV), an emerging enteropathogenic coronavirus, causes diarrhoea in suckling piglets and has the potential for cross-species transmission. No effective PDCoV vaccines or antiviral drugs are currently available.
Puxian Fang, Huichang Zhang, He Sun, Gang Wang, Sijin Xia, Jie Ren, Jiansong Zhang, Liyuan Tian, Liurong Fang, Shaobo Xiao   +9 more
doaj   +1 more source

Generation and validation of a highly sensitive bioluminescent HIV-1 reporter vector that simplifies measurement of virus release

Retrovirology, 2020
Background The continued persistence of HIV-1 as a public health concern due to the lack of a cure calls for the development of new tools for studying replication of the virus.
James Kirui, Eric O. Freed
doaj   +1 more source