Results 31 to 40 of about 174,549 (256)

Novel Mode of nanoLuciferase Packaging in SARS-CoV-2 Virions and VLPs Provides Versatile Reporters for Virus Production

Viruses, 2023
SARS-CoV-2 is a positive-strand RNA virus in the Coronaviridae family that is responsible for morbidity and mortality worldwide. To better understand the molecular pathways leading to SARS-CoV-2 virus assembly, we examined a virus-like particle (VLP ...
Rebekah C. Gullberg, Judith Frydman
doaj   +1 more source

Development of Neutralization Assay Using an eGFP Chikungunya Virus

Viruses, 2016
Chikungunya virus (CHIKV), a member of the Alphavirus genus, is an important human emerging/re-emerging pathogen. Currently, there are no effective antiviral drugs or vaccines against CHIKV infection. Herein, we construct an infectious clone of CHIKV and
Cheng-Lin Deng, Si-Qing Liu, Dong-Gen Zhou, Lin-Lin Xu, Xiao-Dan Li, Pan-Tao Zhang, Peng-Hui Li, Han-Qing Ye, Hong-Ping Wei, Zhi-Ming Yuan, Cheng-Feng Qin, Bo Zhang   +11 more
doaj   +1 more source

Insertion of a GFP Reporter Gene in Influenza Virus [PDF]

Current Protocols in Microbiology, 2013
AbstractThe incorporation of a fluorescent reporter gene into a replication‐competent influenza A virus (IAV) has made it possible to trace IAV infection in vivo. This protocol describes the process of inserting a green fluorescent protein (GFP) reporter into the IAV genome using the established reverse genetics system.
Jasmine T, Perez, Adolfo, García-Sastre, Balaji, Manicassamy   +2 more
openaire   +2 more sources

Generation and Characterization of a Replication-Competent Human Adenovirus Type 55 Encoding EGFP

Viruses, 2023
Human adenovirus 55 (HAdV-55) has recently caused outbreaks of acute respiratory disease (ARD), posing a significant public threat to civilians and military trainees. Efforts to develop antiviral inhibitors and quantify neutralizing antibodies require an
Wei Li, Yuehong Chen, Ye Feng, Jing Li, Xiaoping Kang, Sen Zhang, Yuchang Li, Zhiyan Zhao, Wenguang Yang, Lu Zhao, Huiyao Wang, Tao Jiang   +11 more
doaj   +1 more source

A reporter system for assaying influenza virus RNP functionality based on secreted Gaussia luciferase activity

Virology Journal, 2011
Background Influenza A virus can infect a wide variety of animal species including humans, pigs, birds and other species. Viral ribonucleoprotein (vRNP) was involved in genome replication, transcription and host adaptation. Currently, firefly luciferase (
Wu Xiaobing, Tian Wenhong, Zhu Yun, Yu Zaijiang, Liu Liqi, Du Ning, Qin Kun, Zhou Jianfang, Zhu Wenfei, Shu Yuelong   +9 more
doaj   +1 more source

Modification of H1N1 Influenza Luciferase Reporter Viruses Using StopGo Translation and/or Mouse-Adapted Mutations

Viruses
Reporter viruses are valuable tools for studying infections at the cellular level and in living animals. They also enable rapid, high-throughput antiviral drug screening and serological studies.
Po-Ling Chen, Guohua Yang, Chet Ojha, Balaji Banoth, Charles J. Russell   +4 more
doaj   +1 more source

A cell culture adapted HCV JFH1 variant that increases viral titers and permits the production of high titer infectious chimeric reporter viruses.

PLoS ONE, 2012
The unique properties of the hepatitis C virus (HCV) JFH1 isolate have made it possible to produce and study HCV in an infectious cell culture system. However, relatively low virus titers restrict some of the uses of this system and preparing infectious ...
Shuanghu Liu, Li Xiao, Cassie Nelson, Curt H Hagedorn   +3 more
doaj   +1 more source

Retrospective Analysis of Donor Lymphocyte Infusions in Pediatric Patients With Mixed Chimerism After Hematopoietic Stem Cell Transplantation

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Background Allogeneic hematopoietic stem cell transplantation (alloHSCT) is an essential therapy for several malignant and nonmalignant diseases, but relapse and graft loss remain the principal threats to its success. Routine monitoring of chimerism and minimal residual disease (MRD) enables early detection of imminent recurrence and guides ...
Carmen Junk, Sebastian Michaelis, Michaela Döring, Thomas Eichholz, Ursula Holzer, Martin Ebinger, Tim Flaadt, Rupert Handgretinger, Peter Lang, Jan Frederic Weller, Markus Mezger   +10 more
wiley   +1 more source

Biphasic Clinical Course of Pediatric Cytomegalovirus‐Associated Immune Thrombocytopenia: Early Poor Treatment Response but Favorable Long‐Term Outcomes

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Background Cytomegalovirus (CMV) is a recognized trigger of immune thrombocytopenia (ITP); however, its incidence and impact on disease course in children remain controversial. Therefore, we aimed to characterize the clinical course of pediatric patients with CMV‐associated ITP.
Oded Gilad, Ohad Atia, Osama Tanous, Dafna Brik Simon, Michal Dvori, Miriam Cohen, Tracie A. Goldberg, Efraim Bilavsky, Joanne Yacobovich, Orna Steinberg‐Shemer   +9 more
wiley   +1 more source

VZV Replication Assays

Bio-Protocol, 2014
Varicella zoster virus (VZV) is a human herpesvirus which causes Varicella (chickenpox) upon primary infection and Zoster (shingles) following reactivation from latency (von Bokay, 1909).
Samantha Griffiths, Jürgen Haas
doaj   +1 more source