Results 101 to 110 of about 6,439 (202)

BRET‐Based Approaches for Ion Channels: Emerging Applications and Future Directions

Archiv der Pharmazie, Volume 359, Issue 9, September 2026.
Bioluminescence resonance energy transfer (BRET) is a versatile method for studying ligand–protein interactions. Its compatibility with high‐throughput screening and real‐time kinetics has made it widely used in drug discovery. Here, we summarize recent applications of BRET‐based methods for studying ligand binding to ion channels, highlighting key ...
Nadine Kock, Laura Heitzer, Joana Massa, Marvin Taterra, Marcel Bermúdez, Oliver Koch, Matthias Schiedel   +6 more
wiley   +1 more source

Akaluc bioluminescence offers superior sensitivity to track in vivo dynamics of SARS-CoV-2 infection

iScience
Summary: Monitoring in vivo viral dynamics can improve our understanding of pathogenicity and tissue tropism. Because the gene size of RNA viruses is typically small, NanoLuc is the primary choice for accommodation within viral genome.
Tomokazu Tamura, Hayato Ito, Shiho Torii, Lei Wang, Rigel Suzuki, Shuhei Tsujino, Akifumi Kamiyama, Yoshitaka Oda, Masumi Tsuda, Yuhei Morioka, Saori Suzuki, Kotaro Shirakawa, Kei Sato, Kumiko Yoshimatsu, Yoshiharu Matsuura, Satoshi Iwano, Shinya Tanaka, Takasuke Fukuhara   +17 more
doaj   +1 more source

Nanoluc oligoproteins as a model system for protein misfolding and refolding studies

Biophysical Journal
Protein misfolding can lead to protein malfunction, which may compromise cell viability. Chaperones, including the HSP70 system, are proteins that have evolved to restore the native structure of misfolded proteins. Although most chaperones, including DnaK (bacterial HSP70), were first described over 30 years ago, important questions related to their ...
Grigory V. Mechetin, Piotr E. Marszalek
openaire   +2 more sources

Hemp (Cannabis sativa L.) Seed Protein and Its Hydrolysate Promote Bone Formation via the SMAD‐Dependent BMP Pathway

iNew Medicine, Volume 2, Issue 3, September 2026.
ABSTRACT Objective With the global increase in aging populations, the prevalence of osteoporosis is rising. Current preventive and therapeutic strategies remain limited. The present study aimed to investigate the effects of hemp seed protein (HSP) and its hydrolysis‐derived bioactive peptides (HPH) on osteogenic differentiation and to elucidate their ...
Yan Wang, Jiameng Jia, Yang Yu, Kaiyuan Zheng, Meng Deng, Wei Chen, Guoming Liu, Ping Yi, Dongning Ling, Jing Zhang, Linfeng Liu, Bing Yang, Qing Wu, Ping Wei, Chong Yin   +14 more
wiley   +1 more source

Anticodon-edited tRNA enables translational readthrough of COL4A5 premature termination codons.

PLoS ONE
Alport syndrome is caused by variants in COL4A3, COL4A4, or COL4A5, which encode the α3α4α5 chains of type IV collagen. These variants result in defects in the glomerular basement membrane (GBM) and impaired kidney function.
Kohei Omachi, Joseph J Porter, John D Lueck, Jeffrey H Miner   +3 more
doaj   +1 more source

Proteomic Atlas of Notochordal Cell‐Derived Extracellular Vesicles Highlights EV‐Specific NF‐κB Modulation and Functional Implications of the Protein Corona

Journal of Extracellular Biology, Volume 5, Issue 9, September 2026.
ABSTRACT The notochord is a central signalling hub during embryonic development. After regression of the notochord, notochordal cells (NCs) reside within the developing intervertebral disc. The NC‐secretome, including matrix, proteins and extracellular vesicles (NC‐EVs), promotes regeneration of degenerate intervertebral discs and cartilage. This study
Josette C. van Maanen, Chantal Voskamp, Frank M. Riemers, Harmjan R. Vos, Anouk Eversdijk, Marije Kleinjan, Menno Bergmeijer, Martijn J. C. van Herwijnen, Stuart C. Howes, Frances C. Bach, Tim J. M. Welting, Christine L. Le Maitre, Guus G. H. van den Akker, Marca H. M. Wauben, Mariana A. Tryfonidou   +14 more
wiley   +1 more source

Identification of a Novel Small Molecule Facilitating HIV Elimination by the “Shock‐And‐Kill” Approach

Journal of Medical Virology, Volume 98, Issue 9, September 2026.
ABSTRACT Antiretroviral therapy (ART) has markedly improved the prognosis of people living with HIV (PLWH); however, latent viral reservoirs remain a major barrier to a cure. The “Shock and Kill” strategy aims to reactivate latent provirus with latency‐reversing agents (LRAs) and subsequently eliminate the infected cells, yet most LRAs characterized to
Yuichiro Hara, Haruki Kitamura, Kouki Matsuda, Chieko Fujisaki, Sayaka Sukegawa, Kosuke Tanimoto, Kenji Maeda, Hiroaki Takeuchi   +7 more
wiley   +1 more source

Reporter bacteriophage T7NLC utilizes a novel NanoLuc::CBM fusion for the ultrasensitive detection of Escherichia coli in water. [PDF]

, 2018
Rapid detection of bacteria responsible for foodborne diseases is a growing necessity for public health. Reporter bacteriophages (phages) are robust biorecognition elements uniquely suited for the rapid and sensitive detection of bacterial species.
Singh, S., Le Ny, A-L., Nichols, K.P., Peters, J.E., Talbert, Joey, Garing, S., Nugen, S.R., Hinkley, T.C.   +7 more
core  

Strategies for Target Identification and Mechanism Elucidation in Phytochemicals

Phytochemicals in Food and Medicine, Volume 1, Issue 3, September 2026.
Strategies for Target Identification of Phytochemicals: Labeling Methods VS. Non‐Labeling Methods ABSTRACT Drug targets serve as the fundamental biological basis through which phytochemicals exert their therapeutic effects, and target discovery represents both the core challenge and the rate‐limiting step in elucidating the mechanisms of action of ...
Jialing Chen, Yan Linghu, Dasong Wang, Weidong Pan, Kegang Linghu   +4 more
wiley   +1 more source

Reversible Photocontrol of mRNA Synthesis and Cleavage for mRNA Concentration Switching Inside Artificial Cells

ChemSystemsChem, Volume 8, Issue 5, September 2026.
Azobenzene‐modified hairpin DNA (AzoPinDNA) controls transcription and mRNA cleavage. While visible light (vis) allows transcription by T7 RNA polymerase, UV irradiation triggers AzoPinDNA to open and hybridize with mRNA, inducing site‐specific cleavage by RNase H and inhibiting transcription.
Daichi Iwata, Keiji Murayama, Ken Komiya, Hideaki T. Matsubayashi, Keita Abe, Hiroyuki Asanuma, Satoshi Murata, Shin‐ichiro M. Nomura   +7 more
wiley   +1 more source