Results 101 to 110 of about 5,929 (191)

The long-lasting expression of recombinant artLCMV following intraperitoneal administration exerts potent antitumor effects on tumor models of peritoneal carcinomatosis

OncoImmunology
Peritoneal carcinomatosis remains a challenging clinical condition with limited therapeutic options. In this study, we evaluated the efficacy of a recombinant artLCMV platform encoding tumor antigens and immune-stimulatory molecules in preclinical models.
Celia Gomar, Aline Risson, Leire Arrizabalaga, Nuria Ardaiz, Virginia Belsue, Adrian Gil-Korilis, Sarah Ahmadi-Erber, Timo Schippers, Ignacio Melero, Klaus K. Orlinger, Fernando Aranda, Henning Lauterbach, Pedro Berraondo   +12 more
doaj   +1 more source

Reporter bacteriophage T7NLC utilizes a novel NanoLuc::CBM fusion for the ultrasensitive detection of Escherichia coli in water.

, 2018
Rapid detection of bacteria responsible for foodborne diseases is a growing necessity for public health. Reporter bacteriophages (phages) are robust biorecognition elements uniquely suited for the rapid and sensitive detection of bacterial species.
Singh, S., Le Ny, A-L., Nichols, K.P., Peters, J.E., Talbert, Joey, Garing, S., Nugen, S.R., Hinkley, T.C.   +7 more
core  

NanoLuc Complementation Reporter Optimized for Accurate Measurement of Protein Interactions in Cells

, 2015
Protein-fragment complementation assays (PCAs) are widely used for investigating protein interactions. However, the fragments used are structurally compromised and have not been optimized nor thoroughly characterized for accurately assessing these ...
Kris Zimmerman (420213), Lance P. Encell (420215), Christopher T. Eggers (1262955), Monika G. Wood (1262952), Thomas Machleidt (1262967), Brock F. Binkowski (8915), Mary P. Hall (1262964), Thomas H. Lubben (1262973), Thomas A. Kirkland (1262970), Andrew S. Dixon (1262958), Paul Otto (420212), Marie K. Schwinn (1262961), Keith V. Wood (420217), Braeden L. Butler (420210)   +13 more
core   +1 more source

Rapid generation of HCoV-229E and HCoV-OC43 reporter viruses and replicons for antiviral research

Frontiers in Cellular and Infection Microbiology
IntroductionThe large size of coronavirus genome, along with the instability of certain genomic sequences, makes the construction of reverse genetics for coronaviruses particularly challenging.
Yuyuan Zhang, Jiannan Chen, Hui Feng, Lulu Yang, Liyuan Hu, Yang Gao, Ziqiao Wang, Fei Feng, Jincun Zhao, Ping Zhang, Rong Zhang   +10 more
doaj   +1 more source

Exploiting NanoLuc luciferase for smartphone-based bioluminescence cell biosensor for (anti)-inflammatory activity and toxicity

, 2016
The availability of smartphones with high-performance digital image sensors and processing power has completely reshaped the landscape of point-of-need analysis.
CEVENINI, LUCA, CALABRETTA, MARIA MADDALENA, RODA, ALDO, TASSONI, ANNALISA, TARANTINO, GIUSEPPE, LOPRESIDE, ANTONIA, FERRI, MAURA, MICHELINI, ELISA   +7 more
core   +1 more source

Selenoprotein M Inhibits the Replication of Influenza A Virus by Regulating Reactive Oxygen Species Levels

Life
Background: Influenza A virus (IAV) is the major pathogen responsible for influenza pandemics and can cause seasonal influenza outbreaks. In general, viral infection of host cells increases reactive oxygen species (ROS) levels, a process that triggers ...
Minxuan Liu, Jinhui Wang, Weigang Li, Bo Zhao, Yuanyuan Zhang, Qiaoying Zeng, Guangyuan Liu   +6 more
doaj   +1 more source

Fluorescent Labeling of NanoLuc

, 2015
Bioluminescence is a natural phenomenon that occurs in bacteria, insects, fungi and some marine species whereby light is emitted by a living organism. This emitted light is generated by a chemical reaction that is catalyzed by a class of enzymes known as
DuPlain, Holly
core  

A step by step protocol for absolute quantification of protein using Arabidopsis thaliana transgenic lines carrying NanoLUC-tagged genes [PDF]

Quantification of protein levels in absolute units will allow testing quantitative models of generegulatory networks. Better quantitative models have application in fundamental plant biologybut also in synthetic biology. Models in absolute units can, for
Halliday, Karen J., Urquiza Garcia, Uriel, Molina, Nacho, Millar, Andrew J.; id_orcid   +3 more
core   +1 more source

In vivo bioluminescence imaging of the intracerebral fibroin-controlled AAV-α-synuclein diffusion for monitoring the central nervous system and peripheral expression

Scientific Reports
Among the several animal models of α-synucleinopathies, the well-known viral vector-mediated delivery of wild-type or mutated (A53T) α-synuclein requires new tools to increase the lesion in mice and follow up in vivo expression. To this end, we developed
Claire Mazzocco, Coralie Genevois, Qin Li, Evelyne Doudnikoff, Nathalie Dutheil, Thierry Leste-Lasserre, Marie-Laure Arotcarena, Erwan Bezard   +7 more
doaj   +1 more source

A CAM bioimaging model reveals the connection between VEGFA vascular remodeling and enhanced sarcoma progression via tumor secretome

Scientific Reports
We developed a sensitive bioimaging system for sarcoma by creating a cell line that stably expresses both Katushka2S fluorophore and NanoLuc luciferase, enabling robust dual tracking of tumor growth and metastasis in the chick chorioallantoic membrane ...
Yuzhe Wang, Wenyu Xue, Nawar Sakr, Averyan V. Pushkarev, Elizaveta N. Mochalova, Margarita Pustovalova, Denis V. Kuzmin, Sergey Leonov   +7 more
doaj   +1 more source