Results 61 to 70 of about 5,929 (191)

NanoLuc-based microneutralization assay for high-throughput detection of neutralizing antibodies against rift valley fever virus

MethodsX
Plaque reduction neutralization tests (PRNTs) are the gold standard for measuring neutralizing antibodies but are labor-intensive, low-throughput, and subject to variability from manual plaque counting.
Cigdem Alkan, Alexander N. Freiberg, Tetsuro Ikegami   +2 more
doaj   +1 more source

Construction of oncolytic reporter influenza viral vectors and assessment of their safety following intracranial administration in rats

Microbiology Independent Research Journal
INTRODUCTION: Oncolytic viruses are a promising approach for treating malignant brain tumors as part a of combination therapy. OBJECTIVE: To develop reporter influenza A viruses expressing NanoLuc luciferase and evaluate their safety following ...
A. A. Pulkina, A. S. Mustafaeva, E. A. Romanovskaya-Romanko, M. A. Plotnikova, O. O. Ozhereleva, M. A. Shuklina, L. N. Kiseleva, Y. S. Alekseeva, N. S. Kurmazov, A. R. Muslimov, A.-P.S. Shurygina, M. A. Stukova   +11 more
doaj   +1 more source

Discovery of CRBN‐based molecular glue degraders targeting WIZ transcription factor

Bulletin of the Korean Chemical Society, EarlyView.
This work reports the discovery of 9l, a novel cereblon‐based molecular glue degrader which targets the WIZ transcription factor. Compound 9l facilitates WIZ‐CRBN ternary complex formation to degrade WIZ, subsequently inducing γ‐globin expression in HUDEP‐2 cells and highlighting its potential as a sickle cell disease therapy.
Tae‐Jun Kim, Seongu Seong, Jung‐Ae Kim, Taeyeon Kwon, Hyun Jin Kim, Jeong‐Hoon Kim, Jong Yeon Hwang, Jin Hwa Cho, Kyoungmin Choi   +8 more
wiley   +1 more source

Plasmid: NK588 (pAct-nanoluc) v1

, 2019
The attached file is the full sequence for a plasmid that uses gene regulatory elements from S. rosetta to drive the expression of nanoluc luciferase upon transfection in S. rosetta. The plasmid is backbone is pUC19 and can be propagated in E. coli with Ampicillin selection.
David Booth, Nicole King
openaire   +1 more source

Revolutionizing Healthcare With Paper‐Based Nucleic Acid Testing

Exploration, EarlyView.
This work summarizes strategies to enhance paper‐based devices for nucleic acid testing. Key approaches include optimizing paper platforms, improving nucleic acid amplification, and refining labeling/signaling methods. These advancements aim to boost sensitivity, speed, and usability, making paper‐based diagnostics more effective for point‐of‐care ...
Hong Zhang, Yi Yang, Heng Li, Xiaolin Hu, Hui Xiao, Dou Wang, Sergio Benardini, Wei Gu, Yang Luo   +8 more
wiley   +1 more source

Enhancing Optogenetics‐Based Cancer Therapy Via Nanotechnology

Exploration, EarlyView.
Nanotechnology enhances optogenetics‐based cancer therapy through improved delivery systems and non‐invasive optical regulation. It further enables precise and programmable control of optogenetic living therapeutics for localized antitumor responses.
Honggang Shen, Wenzhe Yi, Rongzhang Li, Shuangshuang Hu, Xindi Qian, Dan Yan, Qian Zou, Guanru Wang, Yaping Li   +8 more
wiley   +1 more source

Lung Cancer Patients Display Increased Expression of Bovine Meat and Milk Factor (BMMF) Proteins in Peritumor Alveolar Macrophages

International Journal of Cancer, EarlyView.
Plasmid‐like DNA sequences frequently found in bovine milk and serum have been proposed as infectious agents promoting indirect carcinogenesis. This multi‐omics study detected immune cells positive for these bovine meat and milk factors (BMMFs) in patients with lung cancer, with BMMF expression inversely correlated with smoking intensity.
Nives Cecere, Kristina Alikhanyan, Claudia Ernst, Lisa Häfele, Lars Feuerbach, Imke Grewe, Sumen Siqin, Karin Gunst, Gunjan Shukla, Veronika Frehtman, Claudia Tessmer, Florian Eichhorn, Michael Allgäuer, Alexander Brobeil, Ilse Hofmann, Barbara Leuchs, Marc A. Schneider, Timo Bund   +17 more
wiley   +1 more source

High-throughput functional analysis provides novel insight into type VII secretion in Staphylococcus aureus

Open Biology
Successful colonization by the opportunistic pathogen Staphylococcus aureus depends on its ability to interact with other microorganisms. Staphylococcus aureus strains harbour a T7b subtype of type VII secretion system (T7SSb), a protein secretion system
Yaping Yang, Aaron A. Scott, Holger Kneuper, Felicity Alcock, Tracy Palmer   +4 more
doaj   +1 more source

Autophagy Plays a Suppressive Role in Bladder Tumor Formation in an Orthotopic Mouse Model and Bladder Cancer Patient Specimens

The Kaohsiung Journal of Medical Sciences, EarlyView.
ABSTRACT Autophagy plays either a suppressing or promoting role during tumor development. Clarifying the role of autophagy in bladder tumorigenesis both in vitro and in vivo is crucial for developing novel therapeutic strategies through manipulating autophagy activity.
Wan‐Ting Kuo, Chin‐Chen Pan, Yi‐Wen Liu, Nan‐Haw Chow, Hong‐Lin Cheng, Shan‐Ying Wu, Sheng‐Hui Lan, Chih‐Peng Chang, Hsiao‐Sheng Liu   +8 more
wiley   +1 more source

Determining the EC50 of Tracer-6908 with ACVR1/ALK2-NanoLuc

, 2018
Promega had provided us with Tracer-6908 that was developed collaboratively with SGC. Before the tracer can be used in nanoBRET assay for compound IC50, it is necessary to determine the optimal tracer concentration.
Wong, Jong Fu, Wong, Jong Fu (5163044), Bullock, Alex, Bullock, Alex (4954282)   +3 more
core   +2 more sources