Results 61 to 70 of about 6,439 (202)

Synthetic Toll‐Like Receptors for Control of Innate Immunity With Far‐Red Light

Advanced Science, EarlyView.
Synthetic Toll‐like receptors (TLRs) are engineered by replacing their natural sensing regions with a far‐red‐light‐responsive module from a bacterial phytochrome. These synthetic receptors enable light‐controlled regulation of immune signaling in mammalian cells.
Anna V. Leopold, Vladislav V. Verkhusha
wiley   +1 more source

Construction of oncolytic reporter influenza viral vectors and assessment of their safety following intracranial administration in rats

Microbiology Independent Research Journal
INTRODUCTION: Oncolytic viruses are a promising approach for treating malignant brain tumors as part a of combination therapy. OBJECTIVE: To develop reporter influenza A viruses expressing NanoLuc luciferase and evaluate their safety following ...
A. A. Pulkina, A. S. Mustafaeva, E. A. Romanovskaya-Romanko, M. A. Plotnikova, O. O. Ozhereleva, M. A. Shuklina, L. N. Kiseleva, Y. S. Alekseeva, N. S. Kurmazov, A. R. Muslimov, A.-P.S. Shurygina, M. A. Stukova   +11 more
doaj   +1 more source

An Integrated Approach toward NanoBRET Tracers for Analysis of GPCR Ligand Engagement

Molecules, 2021
Gaining insight into the pharmacology of ligand engagement with G-protein coupled receptors (GPCRs) under biologically relevant conditions is vital to both drug discovery and basic research.
Michael P. Killoran, Sergiy Levin, Michelle E. Boursier, Kristopher Zimmerman, Robin Hurst, Mary P. Hall, Thomas Machleidt, Thomas A. Kirkland, Rachel Friedman Ohana   +8 more
doaj   +1 more source

Revolutionizing Healthcare With Paper‐Based Nucleic Acid Testing

Exploration, EarlyView.
This work summarizes strategies to enhance paper‐based devices for nucleic acid testing. Key approaches include optimizing paper platforms, improving nucleic acid amplification, and refining labeling/signaling methods. These advancements aim to boost sensitivity, speed, and usability, making paper‐based diagnostics more effective for point‐of‐care ...
Hong Zhang, Yi Yang, Heng Li, Xiaolin Hu, Hui Xiao, Dou Wang, Sergio Benardini, Wei Gu, Yang Luo   +8 more
wiley   +1 more source

Toward Predicting Pandemic Potential: A Comparative Analysis of Virus‐Host Interactions Between Diverse Influenza A Viruses and the Human Innate Immune System

PROTEOMICS, EarlyView.
ABSTRACT Zoonotic infections caused by influenza A viruses (IAVs) of animal origin are a major public health concern, as they may represent the initial step toward the emergence of a pandemic strain. The host range of IAVs is shaped by species‐specific virus‐host interactions, and several host barriers have been identified that restrict the adaptation ...
Antoine Gerodez, Melanie Dos Santos, Mikaël Attia, Killian De Geest, Ibrahim Hamid, Rodrigue Tesse, Ilham Fdillate, Marc Boschmans, Steven Van Borm, Mieke Steensels, Benedicte Lambrecht, Lionel Tafforeau, Caroline Demeret, Cyril Barbezange   +13 more
wiley   +1 more source

High-throughput functional analysis provides novel insight into type VII secretion in Staphylococcus aureus

Open Biology
Successful colonization by the opportunistic pathogen Staphylococcus aureus depends on its ability to interact with other microorganisms. Staphylococcus aureus strains harbour a T7b subtype of type VII secretion system (T7SSb), a protein secretion system
Yaping Yang, Aaron A. Scott, Holger Kneuper, Felicity Alcock, Tracy Palmer   +4 more
doaj   +1 more source

Development of NanoLuc bridging immunoassay for detection of anti-drug antibodies

Journal of Immunological Methods, 2017
Anti-drug antibodies (ADAs) are generated in-vivo as an immune response to therapeutic antibody drugs and can significantly affect the efficacy and safety of the drugs. Hence, detection of ADAs is recommended by regulatory agencies during drug development process.
Nidhi Nath, Rod Flemming, Becky Godat, Marjeta Urh   +3 more
openaire   +2 more sources

Illuminating cellular and extracellular vesicle-mediated communication via a split-Nanoluc reporter in vitro and in vivo [PDF]

, 2023
Tools to effectively demonstrate and quantify functional delivery in cellular communication have been lacking. This study reports the use of a fluorescently labeled split Nanoluc reporter system to demonstrate and quantify functional transfer between ...
Broekman, M.L.D., Breakefield, XO, O'Brien, K.P., Sammarco, A, Sammarco, A., Mahjoum, S, Broekman, MLD, van Solinge, TS, Mahjoum, S., Solinge, T.S. van, Ughetto, S., O'Brien, KP, Breakefield, X.O., Ughetto, S   +13 more
core   +1 more source

Dehydrocostus Lactone Suppresses Hepatocellular Carcinoma by Inhibiting Protein Tyrosine Kinase‐7 Mediated β‐Catenin Signaling

Phytotherapy Research, EarlyView.
Schematic summary of the experimentally supported mechanism of DHL in hepatocellular carcinoma. Dehydrocostus lactone (DHL), a sesquiterpene lactone derived from Aucklandia lappa, directly engages protein tyrosine kinase 7 (PTK7), as supported by molecular docking, CETSA, and DARTS assays.
Xiwen Fan, Junhui Li, Yu Qiao, Yuwen Zhong, Xuecheng Ge, Yaoshuai Zhang, Zishu Wang, Feng Qian, Weixin Ren   +8 more
wiley   +1 more source

Anticuerpos monodominio fusionados a la luciferasa NanoLuc para el diagnóstico de enfermedades infecciosas y alergias [PDF]

En esta tesis se exploró el potencial de generar proteínas quiméricas, formadas por nanobodies unidos a la enzima NanoLuc, para su uso como reactivos de detección en inmunoensayos.
Segovia de los Santos, Paula
core   +7 more sources