Results 31 to 40 of about 5,275 (178)

Activity of Plasmodium vivax promoter elements in Plasmodium knowlesi, and a centromere-containing plasmid that expresses NanoLuc throughout the parasite life cycle

Malaria Journal, 2021
Background Plasmodium knowlesi is now the major cause of human malaria in Malaysia, complicating malaria control efforts that must attend to the elimination of multiple Plasmodium species. Recent advances in the cultivation of P.
Roberto R. Moraes Barros, Kittisak Thawnashom, Tyler J. Gibson, Jennifer S. Armistead, Ramoncito L. Caleon, Miho Kaneko, Whitney A. Kite, J. Patrick Mershon, Jacqueline K. Brockhurst, Theresa Engels, Lynn Lambert, Sachy Orr-Gonzalez, John H. Adams, Juliana M. Sá, Osamu Kaneko, Thomas E. Wellems   +15 more
doaj   +1 more source

Glutathione‐Responsive Acyl‐Modifications for Targeted RNA Decaging and Prolonged Protein Synthesis

Angewandte Chemie, EarlyView.
The self‐immolation of disulfide‐based mRNA modifications in response to endogenous glutathione (GSH) promotes a gradual release of translatable mRNA within the cell, leading to improved nuclease resistance, tunable release properties, and a significant increase (up to 600%) of target protein production over time. This strategy offers an exciting proof
Mary E. Flood, Sathishkumar Kurusamy, Mark Berney, C. Mark Smales, Elizabeth M. Topp, Joanna F. McGouran, Steven Ferguson   +6 more
wiley   +2 more sources

Luciferase complementation based-detection of G-protein-coupled receptor activity

BioTechniques, 2018
Protein complementation assays (PCA) are used as pharmacological tools, enabling a wide array of applications, ranging from studies of protein–protein interactions to second messenger effects.
Hideaki Yano, Ning Sheng Cai, Jonathan A Javitch, Sergi Ferré   +3 more
doaj   +1 more source

Development of NanoLuc-targeting protein degraders and a universal reporter system to benchmark tag-targeted degradation platforms

Nature Communications, 2022
tag-Targeted Protein Degrader (tTPD) systems are powerful tools for preclinical target validation. Here the authors extend the tTPD platform by developing NanoTACs that degrade NanoLuc tagged substrates and benchmark each tTPD system using an ...
Christoph Grohmann, Charlene M. Magtoto, Joel R. Walker, Ngee Kiat Chua, Anna Gabrielyan, Mary Hall, Simon A. Cobbold, Stephen Mieruszynski, Martin Brzozowski, Daniel S. Simpson, Hao Dong, Bridget Dorizzi, Annette V. Jacobsen, Emma Morrish, Natasha Silke, James M. Murphy, Joan K. Heath, Andrea Testa, Chiara Maniaci, Alessio Ciulli, Guillaume Lessene, John Silke, Rebecca Feltham   +22 more
doaj   +1 more source

Ratiometric BRET Measurements of ATP with a Genetically-Encoded Luminescent Sensor

Sensors, 2019
Luciferase-based reporters provide a key measurement approach in a broad range of applications, from in vitro high-throughput screening to whole animal imaging.
Se-Hong Min, Alexander R. French, Keelan J. Trull, Kiet Tat, S. Ashley Varney, Mathew Tantama   +5 more
doaj   +1 more source

Sensing chemical-induced genotoxicity and oxidative stress via yeast-based reporter assays using NanoLuc luciferase.

PLoS ONE, 2023
Mutagens and oxidative agents damage biomolecules, such as DNA; therefore, detecting genotoxic and oxidative chemicals is crucial for maintaining human health.
Minami Shichinohe, Shun Ohkawa, Yuu Hirose, Toshihiko Eki   +3 more
doaj   +1 more source

Mechanical Stability of a Small, Highly-Luminescent Engineered Protein NanoLuc [PDF]

International Journal of Molecular Sciences, 2020
NanoLuc is a bioluminescent protein recently engineered for applications in molecular imaging and cellular reporter assays. Compared to other bioluminescent proteins used for these applications, like Firefly Luciferase and Renilla Luciferase, it is ~150 times brighter, more thermally stable, and smaller. Yet, no information is known with regards to its
Yue Ding, Dimitra Apostolidou, Piotr Marszalek   +2 more
openaire   +2 more sources

Dual Reporter Bioluminescence Imaging with NanoLuc and Firefly Luciferase [PDF]

, 2018
Bioluminescence imaging is a powerful, broadly utilized method for noninvasive imaging studies in cell-based assays and small animal models of normal physiology and multiple diseases. In combination with molecular engineering of cells and entire organisms using luciferase enzymes, bioluminescence imaging has enabled novel applications including studies
Anne E, Gibbons, Kathryn E, Luker, Gary D, Luker   +2 more
openaire   +2 more sources

Internal light source for deep photodynamic therapy

Light: Science & Applications, 2022
Photodynamic therapy (PDT) for deep-seated lesion is seriously hindered by the limited depth of visible light penetration. Most recently, researchers have designed a genetically-encoded NanoLuc-miniSOG with internal light source for self-excitation ...
Buhong Li, Li Lin
doaj   +1 more source

An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes

FEBS Letters, EarlyView.
GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg, Martine Hjeltnes, Josephine Krieger, Joshua E. Elias, Maria Stensland, Kristian Holm, Xiaojun Jiang, Chuki Khangsar, Tuula A. Nyman, Arthur Kaser, Johannes R. Hov, Tom H. Karlsen, Nicole C. Kaneider, Frode L. Jahnsen, Georg Schneditz   +14 more
wiley   +1 more source