Novel Bioluminescent Binding Assays for Ligand-Receptor Interaction Studies of the Fibroblast Growth Factor Family. [PDF]
PLoS ONE, 2016 We recently developed novel bioluminescent binding assays for several protein/peptide hormones to study their interactions with receptors using the so far brightest NanoLuc reporter.
Ge Song +5 more
doaj +1 more source
Rescue of NanoLuc luciferase-expressing Senecavirus A with oncolytic activity
Virus Research, 2021 Senecavirus A (SVA), previously known as Seneca Valley virus, is classified into the genus Senecavirus in the family Picornaviridae. SVA is not pathogenic to normal human cells, but has potent oncolytic activity in some tumor cells with neuroendocrine feature, such as small cell lung cancer (SCLC) NCI-H446 cell line.
Fuxiao, Liu +4 more
openaire +2 more sources
Expression of NanoLuc Luciferase in Listeria innocua for Development of Biofilm Assay [PDF]
Frontiers in Microbiology, 2021 Studies of biofilm formation by bacteria are crucial for understanding bacterial resistance and for development of novel antibacterial strategies. We have developed a new bioluminescence biofilm assay for Listeria innocua, which is considered a non-pathogenic surrogate for Listeria monocytogenes. L.
Aleš Berlec +5 more
openaire +3 more sources
Split luciferase-based assay to detect botulinum neurotoxins using hiPSC-derived motor neurons
Communications Biology, 2023 A cell-based assay for detecting SNAP25-cleaving Botulinum neurotoxins (BoNTs) is developed by combining hiPSC-derived motor neurons and a luminescent detection system based on split NanoLuc luciferase.
Laurent Cotter +6 more
doaj +1 more source
Nghiên cứu tạo protein tín hiệu nanoluciferase: Ứng dụng tạo cảm biến sinh học nhận diện kháng sinh
Tạp chí Khoa học Đại học Cần Thơ, 2020 Nghiên cứu được thực hiện nhằm tổng hợp protein tín hiệu nanoluciferase trong điều kiện phòng thí nghiệm (in vitro), sử dụng hệ thống phiên mã dịch mã ngoài tế bào (cell-free synthesis) để ứng dụng tạo cảm biến sinh học nhận diện nhóm kháng sinh ức chế ...
Trần Thị Mỹ Duyên +1 more
doaj +3 more sources
A Bioluminescent Sensor for Rapid Detection of PPEP-1, a Clostridioides difficile Biomarker
Sensors, 2021 Current assays for Clostridioides difficile in nonhospital settings are outsourced and time-intensive, resulting in both delayed diagnosis and quarantining of infected individuals.
Kevin K. Ng +5 more
doaj +1 more source
NanoLuc Reporter for Dual Luciferase Imaging in Living Animals [PDF]
Molecular Imaging, 2013 Bioluminescence imaging is widely used for cell-based assays and animal imaging studies in biomedical research and drug development, capitalizing on the high signal to background of this technique. A relatively small number of luciferases are available for imaging studies, substantially limiting the ability to image multiple molecular and cellular ...
Amanda C. Stacer +6 more
openaire +3 more sources
An ultrasensitive NanoLuc-based luminescence system for monitoring Plasmodium berghei throughout its life cycle [PDF]
, 2016 BACKGROUND: Bioluminescence imaging is widely used for cell-based assays and animal imaging studies, both in biomedical research and drug development.
Derya Keller +12 more
core +3 more sources
, 2020 NanoLuc luminescence measured in leaves infiltrated with p2X35S-NanoLuc-NOSt, p19 and a NanoLuc editing construct. Percentage reduction of luminescence due to editing of NanoLuc, calculated by setting luminescence of control leaves (infiltrated with ...
Satomi Hayashi (3575801) +7 more
core +1 more source
Engineering new tools and imaging platforms with NanoLuc
, 2022 Bioluminescence imaging is a powerful modality for probing cellular and molecular features in vivo. This technology involves a luciferase enzyme that oxidizes a small molecule luciferin, thereby producing light. The recent development of NanoLuc, a small,
Ng, Kevin King-Yiu
core +1 more source