Results 21 to 30 of about 5,929 (191)

Anticodon-edited tRNA enables translational readthrough of COL4A5 premature termination codons. [PDF]

open access: yesPLoS ONE
Alport syndrome is caused by variants in COL4A3, COL4A4, or COL4A5, which encode the α3α4α5 chains of type IV collagen. These variants result in defects in the glomerular basement membrane (GBM) and impaired kidney function.
Kohei Omachi   +3 more
doaj   +2 more sources

NanoLuc as a tool to study infection with a plant virus

open access: yes, 2018
NanoLuc as a tool to study infection with a plant virus. 50. Jahrestreffen des Arbeitskreises "Viruskrankheiten der Pflanzen"
Ducousso, Marie   +3 more
core   +4 more sources

Bioluminescent Sensors for Ca++ Flux Imaging and the Introduction of a New Intensity-Based Ca++ Sensor

open access: yesFrontiers in Bioengineering and Biotechnology, 2021
Sensitive detection of biological events is a goal for the design and characterization of sensors that can be used in vitro and in vivo. One important second messenger is Ca++ which has been a focus of using genetically encoded Ca++ indicators (GECIs ...
Jie Yang, Carl Hirschie Johnson
doaj   +1 more source

A Slow but Steady NanoLuc: R162A mutation results in a decreased, but stable, NanoLuc activity

open access: yesInternational Journal of Biological Macromolecules, 2023
Abstract NanoLuc (NLuc) luciferase has found extensive application in designing a range of biological assays including gene expression analysis, protein-protein interaction and protein conformational changes due to its enhanced brightness and small size.
Wesam S. Ahmed   +6 more
openaire   +2 more sources

Real-Time Visualization of the Infection and Replication of a Mouse-Lethal Recombinant H9N2 Avian Influenza Virus

open access: yesFrontiers in Veterinary Science, 2022
H9N2 avian influenza viruses (AIVs) continuously cross the species barrier to infect mammalians and are repeatedly transmitted to humans, posing a significant threat to public health.
Guangjie Lao   +17 more
doaj   +1 more source

Cải thiện cảm biến sinh học nhận diện nhóm kháng sinh ức chế sự tổng hợp protein của vi khuẩn

open access: yesTạp chí Khoa học Đại học Cần Thơ, 2023
Nghiên cứu được thực hiện nhằm cải thiện độ nhạy của cảm biến sinh học nhận diện nhóm kháng sinh ức chế sự tổng hợp protein trên nền giấy. Cảm biến sinh học nhận diện kháng sinh dựa trên cường độ phát quang của protein nanoluciferase (NanoLuc) được tổng
Trần Thị Mỹ Duyên   +1 more
doaj   +1 more source

Generation and validation of a highly sensitive bioluminescent HIV-1 reporter vector that simplifies measurement of virus release

open access: yesRetrovirology, 2020
Background The continued persistence of HIV-1 as a public health concern due to the lack of a cure calls for the development of new tools for studying replication of the virus.
James Kirui, Eric O. Freed
doaj   +1 more source

NanoLuc-encoding artificial intron sequence.

open access: yes, 2021
(TIF)
Kaitlyn N. Burke (10885460)   +6 more
core   +1 more source

NanoLuc luciferase as a quantitative yeast two-hybrid reporter

open access: yesFEMS Yeast Research, 2021
ABSTRACT The yeast two-hybrid (Y2H) assay is a powerful technique to identify protein-protein interactions. However, the auxotrophic markers that are the most common Y2H reporters take several days to yield data and require subjective assessment of semiquantitative data to identify interactions.
Veronica J, Heintz   +2 more
openaire   +2 more sources

NanoLuc Reporter for Dual Luciferase Imaging in Living Animals [PDF]

open access: yesMolecular Imaging, 2013
Bioluminescence imaging is widely used for cell-based assays and animal imaging studies in biomedical research and drug development, capitalizing on the high signal to background of this technique. A relatively small number of luciferases are available for imaging studies, substantially limiting the ability to image multiple molecular and cellular ...
Amanda C. Stacer   +6 more
openaire   +3 more sources

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