Results 21 to 30 of about 5,275 (178)
EBioMedicine, 2020 Background: Many flaviviruses are significant human pathogens that cause global public health threats. Developing research tools for studying and diagnosing these pathogens is a top priority.
Coleman Baker +5 more
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Novel Bioluminescent Binding Assays for Ligand-Receptor Interaction Studies of the Fibroblast Growth Factor Family. [PDF]
PLoS ONE, 2016 We recently developed novel bioluminescent binding assays for several protein/peptide hormones to study their interactions with receptors using the so far brightest NanoLuc reporter.
Ge Song +5 more
doaj +1 more source
NanoLuc Reporter for Dual Luciferase Imaging in Living Animals [PDF]
Molecular Imaging, 2013 Bioluminescence imaging is widely used for cell-based assays and animal imaging studies in biomedical research and drug development, capitalizing on the high signal to background of this technique. A relatively small number of luciferases are available for imaging studies, substantially limiting the ability to image multiple molecular and cellular ...
Amanda C. Stacer +6 more
openaire +3 more sources
NanoLuc: A Small Luciferase Is Brightening Up the Field of Bioluminescence [PDF]
Bioconjugate Chemistry, 2016 The biomedical field has greatly benefited from the discovery of bioluminescent proteins. Currently, scientists employ bioluminescent systems for numerous biomedical applications, ranging from highly sensitive cellular assays to bioluminescence-based molecular imaging.
Christopher G, England +2 more
openaire +2 more sources
Rescue of NanoLuc luciferase-expressing Senecavirus A with oncolytic activity
Virus Research, 2021 Senecavirus A (SVA), previously known as Seneca Valley virus, is classified into the genus Senecavirus in the family Picornaviridae. SVA is not pathogenic to normal human cells, but has potent oncolytic activity in some tumor cells with neuroendocrine feature, such as small cell lung cancer (SCLC) NCI-H446 cell line.
Fuxiao, Liu +4 more
openaire +2 more sources
Split luciferase-based assay to detect botulinum neurotoxins using hiPSC-derived motor neurons
Communications Biology, 2023 A cell-based assay for detecting SNAP25-cleaving Botulinum neurotoxins (BoNTs) is developed by combining hiPSC-derived motor neurons and a luminescent detection system based on split NanoLuc luciferase.
Laurent Cotter +6 more
doaj +1 more source
Nghiên cứu tạo protein tín hiệu nanoluciferase: Ứng dụng tạo cảm biến sinh học nhận diện kháng sinh
Tạp chí Khoa học Đại học Cần Thơ, 2020 Nghiên cứu được thực hiện nhằm tổng hợp protein tín hiệu nanoluciferase trong điều kiện phòng thí nghiệm (in vitro), sử dụng hệ thống phiên mã dịch mã ngoài tế bào (cell-free synthesis) để ứng dụng tạo cảm biến sinh học nhận diện nhóm kháng sinh ức chế ...
Trần Thị Mỹ Duyên +1 more
doaj +3 more sources
A Bioluminescent Sensor for Rapid Detection of PPEP-1, a Clostridioides difficile Biomarker
Sensors, 2021 Current assays for Clostridioides difficile in nonhospital settings are outsourced and time-intensive, resulting in both delayed diagnosis and quarantining of infected individuals.
Kevin K. Ng +5 more
doaj +1 more source
Cell Communication and Signaling, 2023 The dimerization of RAF kinases represents a key event in their activation cycle and in RAS/ERK pathway activation. Genetic, biochemical and structural approaches provided key insights into this process defining RAF signaling output and the clinical ...
Lino Rohrer +6 more
doaj +1 more source
iScience, 2022 Summary: Alport syndrome, a disease of kidney, ear, and eye, is caused by pathogenic variants in the COL4A3, COL4A4, or COL4A5 genes encoding collagen α3α4α5(IV) of basement membranes.
Kohei Omachi +3 more
doaj +1 more source