Results 21 to 30 of about 6,439 (202)

Bright Red Bioluminescence from Semisynthetic NanoLuc (sNLuc)

ACS Chemical Biology
Red-shifted bioluminescence is highly desirable for diagnostic and imaging applications. Herein, we report a semisynthetic NanoLuc (sNLuc) based on complementation of a split NLuc (LgBiT) with a synthetic peptide (SmBiT) functionalized with a fluorophore for BRET emission.
Russo, Francesco, Civili, Beatrice, Winssinger, Nicolas   +2 more
openaire   +3 more sources

NanoLuc as a tool to study infection with a plant virus

, 2018
NanoLuc as a tool to study infection with a plant virus. 50.
Buissinot, Sylvaine, Brault, Véronique, Ducousso, Marie, Drucker, Martin   +3 more
core   +5 more sources

Engineering with NanoLuc: a playground for the development of bioluminescent protein switches and sensors

Biochemical Society Transactions, 2020
The small engineered luciferase NanoLuc has rapidly become a powerful tool in the fields of biochemistry, chemical biology, and cell biology due to its exceptional brightness and stability. The continuously expanding NanoLuc toolbox has been employed in applications ranging from biosensors to molecular and cellular imaging, and currently includes split
Lieuwe Biewenga, Bas J.H.M. Rosier, Maarten Merkx   +2 more
openaire   +3 more sources

An optimized luciferin formulation for NanoLuc-based in vivo bioluminescence imaging

Scientific Reports
Bioluminescence imaging (BLI) is widely used in preclinical biomedical research for noninvasive tracking of cell populations and biochemical events in vivo.
Chao Gao, Yan Wu, Connor Fitzgerald, Hui Wang, Tim Ugo, Tetsuo Uyeda, Wenhui Zhou, Yichi Su, Thomas A. Kirkland, Michael Z. Lin   +9 more
doaj   +3 more sources

A Slow but Steady NanoLuc: R162A mutation results in a decreased, but stable, NanoLuc activity

International Journal of Biological Macromolecules, 2023
Abstract NanoLuc (NLuc) luciferase has found extensive application in designing a range of biological assays including gene expression analysis, protein-protein interaction and protein conformational changes due to its enhanced brightness and small size.
Wesam S. Ahmed, Anupriya M. Geethakumari, Asfia Sultana, Asma Fatima, Angelin M. Philip, S.M. Nasir Uddin, Kabir H. Biswas   +6 more
openaire   +2 more sources

Illuminating the mechanism and allosteric behavior of NanoLuc luciferase

Nature Communications, 2022
Abstract NanoLuc, a superior β-barrel fold luciferase, was engineered 10 years ago but the nature of its catalysis remains puzzling. Here experimental and computational techniques were combined, revealing that imidazopyrazinone luciferins bind to an intra-barrel catalytic site but also to an allosteric site shaped on ...
Michal Nemergut, Daniel Pluskal, Jana Horackova, Tereza Sustrova, Jan Tulis, Tomas Barta, Racha Baatallah, Glwadys Gagnot, Veronika Novakova, Marika Majerova, Karolina Sedlackova, Sérgio M. Marques, Martin Toul, Jiri Damborsky, Zbynek Prokop, David Bednar, Yves L. Janin, Martin Marek   +17 more
openaire   +5 more sources

NanoLuc-encoding artificial intron sequence.

, 2021
(TIF)
Kaitlyn N. Burke (10885460), Xinyu Zhu (579718), Nicholas S. Heaton (7435301), Ryan R. Chaparian (10885457), Benjamin S. Chambers (7871783), Heather M. Froggatt (11485853), Hector A. Miranda (11485856)   +6 more
core   +1 more source

NanoLuc luciferase as a quantitative yeast two-hybrid reporter

FEMS Yeast Research, 2021
ABSTRACT The yeast two-hybrid (Y2H) assay is a powerful technique to identify protein-protein interactions. However, the auxotrophic markers that are the most common Y2H reporters take several days to yield data and require subjective assessment of semiquantitative data to identify interactions.
Veronica J, Heintz, Ling, Wang, Douglas J, LaCount   +2 more
openaire   +2 more sources

NanoLuc: A Small Luciferase Is Brightening Up the Field of Bioluminescence [PDF]

Bioconjugate Chemistry, 2016
The biomedical field has greatly benefited from the discovery of bioluminescent proteins. Currently, scientists employ bioluminescent systems for numerous biomedical applications, ranging from highly sensitive cellular assays to bioluminescence-based molecular imaging.
Christopher G, England, Emily B, Ehlerding, Weibo, Cai   +2 more
openaire   +2 more sources

Using recombination-dependent lethal mutations to stabilize reporter flaviviruses for rapid serodiagnosis and drug discovery

EBioMedicine, 2020
Background: Many flaviviruses are significant human pathogens that cause global public health threats. Developing research tools for studying and diagnosing these pathogens is a top priority.
Coleman Baker, Xuping Xie, Jing Zou, Antonio Muruato, Katja Fink, Pei-Yong Shi   +5 more
doaj   +1 more source