Expanding the bioluminescent reporter toolkit for plant science with NanoLUC [PDF]
Plant Methods, 2019 Background Protein data over circadian time scale is scarce for clock transcription factors. Further work in this direction is required for refining quantitative clock models.
Uriel Urquiza-García, Andrew J. Millar
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NanoLuc luciferase - A multifunctional tool for high throughput antibody screening [PDF]
Frontiers in Pharmacology, 2016 Based on the recent development of NanoLuc Luciferase a small (19 kDa), highly stable, ATP independent, bioluminescent protein, an extremely robust and ultra high sensitivity screening system has been developed whereby primary hits of therapeutic ...
Nicolas eBoute +5 more
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Improved HaloTag Ligand Enables BRET Imaging With NanoLuc [PDF]
Frontiers in Chemistry, 2020 Bioluminescence resonance energy transfer (BRET) from an exceptionally bright luciferase, NanoLuc, to a fluorescent HaloTag ligand is gaining momentum to monitor molecular interactions.
Ovia Margaret Thirukkumaran +8 more
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Coelenterazine analogues emit red-shifted bioluminescence with NanoLuc [PDF]
Org. Biomol. Chem., 2017 Synthesis and biological investigation of coelenterazine analogs that demonstrate a tunable red-shift in their bioluminescent emission with NanoLuc luciferase.
Anton Shakhmin +5 more
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Campylobacter jejuni Biofilm Assessment by NanoLuc Luciferase Assay [PDF]
Bio-ProtocolCampylobacter jejuni, a widespread pathogen found in birds and mammals, poses a significant risk for zoonosis worldwide despite its susceptibility to environmental and food-processing stressors.
Tjaša Čukajne +5 more
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Mechanical Stability of a Small, Highly-Luminescent Engineered Protein NanoLuc [PDF]
International Journal of Molecular Sciences, 2020 NanoLuc is a bioluminescent protein recently engineered for applications in molecular imaging and cellular reporter assays. Compared to other bioluminescent proteins used for these applications, like Firefly Luciferase and Renilla Luciferase, it is ~150 times brighter, more thermally stable, and smaller. Yet, no information is known with regards to its
Yue Ding +2 more
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Engineering and exploiting synthetic allostery of NanoLuc luciferase [PDF]
Nature Communications, 2022 Allosteric regulation of proteins allows transduction and interconversion of biochemical signals. Here the authors construct an allosteric version of circular permutated NanoLuc that can be activated by a ligand; they use this to create biosensors to ...
Zhong Guo +10 more
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Holistic monitoring of Campylobacter jejuni biofilms with NanoLuc bioluminescence [PDF]
Applied Microbiology and BiotechnologyCampylobacter jejuni, a major cause of foodborne zoonotic infections worldwide, shows a paradoxical ability to survive despite its susceptibility to environmental and food-processing stressors. This resilience is likely due to the bacterium entering a viable but non-culturable state, often within biofilms, or even initiating biofilm formation as a ...
Tjaša Čukajne +5 more
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Listeria innocua Biofilm Assay Using NanoLuc Luciferase
Bio-Protocol, 2022 Biofilms serve as a bacterial survival strategy, allowing bacteria to persist under adverse environmental conditions. The non-pathogenic Listeria innocua is used as a surrogate organism for the foodborne pathogen Listeria monocytogenes, because they ...
Aleš Berlec +4 more
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Development of NanoLuc-targeting protein degraders and a universal reporter system to benchmark tag-targeted degradation platforms [PDF]
Nature Communications, 2022 tag-Targeted Protein Degrader (tTPD) systems are powerful tools for preclinical target validation. Here the authors extend the tTPD platform by developing NanoTACs that degrade NanoLuc tagged substrates and benchmark each tTPD system using an ...
Christoph Grohmann +22 more
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