Results 11 to 20 of about 5,929 (191)

Campylobacter jejuni Biofilm Assessment by NanoLuc Luciferase Assay

Bio-Protocol
Campylobacter jejuni, a widespread pathogen found in birds and mammals, poses a significant risk for zoonosis worldwide despite its susceptibility to environmental and food-processing stressors.
Tjaša Čukajne, Petra Štravs, Orhan Sahin, Qijing Zhang, Aleš Berlec, Anja Klančnik   +5 more
doaj   +6 more sources

Coelenterazine analogues emit red-shifted bioluminescence with NanoLuc [PDF]

Org. Biomol. Chem., 2017
Synthesis and biological investigation of coelenterazine analogs that demonstrate a tunable red-shift in their bioluminescent emission with NanoLuc luciferase.
Anton Shakhmin, Mary P. Hall, Thomas Machleidt, Joel R. Walker, Keith V. Wood, Thomas A. Kirkland   +5 more
core   +9 more sources

A versatile Plasmodium falciparum reporter line expressing NanoLuc enables highly sensitive multi-stage drug assays

Communications Biology, 2023
Transgenic luciferase-expressing Plasmodium falciparum parasites have been widely used for the evaluation of anti-malarial compounds. Here, to screen for anti-malarial drugs effective against multiple stages of the parasite, we generate a P.
Yukiko Miyazaki, Martijn W. Vos, Fiona J. A. Geurten, Pierre Bigeard, Hans Kroeze, Shohei Yoshioka, Mitsuhiro Arisawa, Daniel Ken Inaoka, Valerie Soulard, Koen J. Dechering, Blandine Franke-Fayard, Shinya Miyazaki   +11 more
doaj   +2 more sources

Illuminating the mechanism and allosteric behavior of NanoLuc luciferase

Nature Communications, 2022
Abstract NanoLuc, a superior β-barrel fold luciferase, was engineered 10 years ago but the nature of its catalysis remains puzzling. Here experimental and computational techniques were combined, revealing that imidazopyrazinone luciferins bind to an intra-barrel catalytic site but also to an allosteric site shaped on ...
Michal Nemergut, Daniel Pluskal, Jana Horackova, Tereza Sustrova, Jan Tulis, Tomas Barta, Racha Baatallah, Glwadys Gagnot, Veronika Novakova, Marika Majerova, Karolina Sedlackova, Sérgio M. Marques, Martin Toul, Jiri Damborsky, Zbynek Prokop, David Bednar, Yves L. Janin, Martin Marek   +17 more
core   +6 more sources

Mechanical Stability of a Small, Highly-Luminescent Engineered Protein NanoLuc [PDF]

International Journal of Molecular Sciences, 2020
NanoLuc is a bioluminescent protein recently engineered for applications in molecular imaging and cellular reporter assays. Compared to other bioluminescent proteins used for these applications, like Firefly Luciferase and Renilla Luciferase, it is ~150 times brighter, more thermally stable, and smaller. Yet, no information is known with regards to its
Yue Ding, Dimitra Apostolidou, Piotr Marszalek   +2 more
openaire   +3 more sources

Development of NanoLuc-PEST expressing Leishmania mexicana as a new drug discovery tool for axenic- and intramacrophage-based assays. [PDF]

PLoS Neglected Tropical Diseases, 2018
The protozoan parasite Leishmania causes leishmaniasis; a spectrum of diseases of which there are an estimated 1 million new cases each year. Current treatments are toxic, expensive, difficult to administer, and resistance to them is emerging.
Sarah L Berry, Hamza Hameed, Anna Thomason, Marissa L Maciej-Hulme, Somaia Saif Abou-Akkada, Paul Horrocks, Helen P Price   +6 more
doaj   +3 more sources

Holistic monitoring of Campylobacter jejuni biofilms with NanoLuc bioluminescence

Applied Microbiology and Biotechnology
Campylobacter jejuni, a major cause of foodborne zoonotic infections worldwide, shows a paradoxical ability to survive despite its susceptibility to environmental and food-processing stressors. This resilience is likely due to the bacterium entering a viable but non-culturable state, often within biofilms, or even initiating biofilm formation as a ...
Tjaša Čukajne, Petra Štravs, Orhan Sahin, Qijing Zhang, Aleš Berlec, Anja Klančnik   +5 more
openaire   +5 more sources

Development of NanoLuc-targeting protein degraders and a universal reporter system to benchmark tag-targeted degradation platforms [PDF]

Nature Communications, 2022
tag-Targeted Protein Degrader (tTPD) systems are powerful tools for preclinical target validation. Here the authors extend the tTPD platform by developing NanoTACs that degrade NanoLuc tagged substrates and benchmark each tTPD system using an ...
Christoph Grohmann, Charlene M. Magtoto, Joel R. Walker, Ngee Kiat Chua, Anna Gabrielyan, Mary Hall, Simon A. Cobbold, Stephen Mieruszynski, Martin Brzozowski, Daniel S. Simpson, Hao Dong, Bridget Dorizzi, Annette V. Jacobsen, Emma Morrish, Natasha Silke, James M. Murphy, Joan K. Heath, Andrea Testa, Chiara Maniaci, Alessio Ciulli, Guillaume Lessene, John Silke, Rebecca Feltham   +22 more
doaj   +4 more sources

Bright Red Bioluminescence from Semisynthetic NanoLuc (sNLuc)

ACS Chemical Biology
Red-shifted bioluminescence is highly desirable for diagnostic and imaging applications. Herein, we report a semisynthetic NanoLuc (sNLuc) based on complementation of a split NLuc (LgBiT) with a synthetic peptide (SmBiT) functionalized with a fluorophore for BRET emission.
Russo, Francesco, Civili, Beatrice, Winssinger, Nicolas   +2 more
openaire   +3 more sources

An optimized luciferin formulation for NanoLuc-based in vivo bioluminescence imaging

Scientific Reports
Bioluminescence imaging (BLI) is widely used in preclinical biomedical research for noninvasive tracking of cell populations and biochemical events in vivo.
Chao Gao, Yan Wu, Connor Fitzgerald, Hui Wang, Tim Ugo, Tetsuo Uyeda, Wenhui Zhou, Yichi Su, Thomas A. Kirkland, Michael Z. Lin   +9 more
doaj   +3 more sources