Results 11 to 20 of about 5,275 (178)
Engineering and exploiting synthetic allostery of NanoLuc luciferase [PDF]
Nature Communications, 2022 Allosteric regulation of proteins allows transduction and interconversion of biochemical signals. Here the authors construct an allosteric version of circular permutated NanoLuc that can be activated by a ligand; they use this to create biosensors to ...
Zhong Guo +10 more
doaj +6 more sources
Expanding the bioluminescent reporter toolkit for plant science with NanoLUC [PDF]
Plant Methods, 2019 Background Protein data over circadian time scale is scarce for clock transcription factors. Further work in this direction is required for refining quantitative clock models.
Uriel Urquiza-García, Andrew J. Millar
doaj +4 more sources
Listeria innocua Biofilm Assay Using NanoLuc Luciferase
Bio-Protocol, 2022 Biofilms serve as a bacterial survival strategy, allowing bacteria to persist under adverse environmental conditions. The non-pathogenic Listeria innocua is used as a surrogate organism for the foodborne pathogen Listeria monocytogenes, because they ...
Aleš Berlec +4 more
doaj +3 more sources
Campylobacter jejuni Biofilm Assessment by NanoLuc Luciferase Assay
Bio-ProtocolCampylobacter jejuni, a widespread pathogen found in birds and mammals, poses a significant risk for zoonosis worldwide despite its susceptibility to environmental and food-processing stressors.
Tjaša Čukajne +5 more
doaj +5 more sources
An optimized luciferin formulation for NanoLuc-based in vivo bioluminescence imaging
Scientific ReportsBioluminescence imaging (BLI) is widely used in preclinical biomedical research for noninvasive tracking of cell populations and biochemical events in vivo.
Chao Gao +9 more
doaj +3 more sources
A Slow but Steady NanoLuc: R162A mutation results in a decreased, but stable, NanoLuc activity
International Journal of Biological Macromolecules, 2023 Abstract NanoLuc (NLuc) luciferase has found extensive application in designing a range of biological assays including gene expression analysis, protein-protein interaction and protein conformational changes due to its enhanced brightness and small size.
Wesam S. Ahmed +6 more
openaire +2 more sources
Illuminating the mechanism and allosteric behavior of NanoLuc luciferase
Nature Communications, 2022 Abstract NanoLuc, a superior β-barrel fold luciferase, was engineered 10 years ago but the nature of its catalysis remains puzzling. Here experimental and computational techniques were combined, revealing that imidazopyrazinone luciferins bind to an intra-barrel catalytic site but also to an allosteric site shaped on ...
Michal Nemergut +17 more
openaire +4 more sources
Cải thiện cảm biến sinh học nhận diện nhóm kháng sinh ức chế sự tổng hợp protein của vi khuẩn
Tạp chí Khoa học Đại học Cần Thơ, 2023 Nghiên cứu được thực hiện nhằm cải thiện độ nhạy của cảm biến sinh học nhận diện nhóm kháng sinh ức chế sự tổng hợp protein trên nền giấy. Cảm biến sinh học nhận diện kháng sinh dựa trên cường độ phát quang của protein nanoluciferase (NanoLuc) được tổng
Trần Thị Mỹ Duyên +1 more
doaj +1 more source
Retrovirology, 2020 Background The continued persistence of HIV-1 as a public health concern due to the lack of a cure calls for the development of new tools for studying replication of the virus.
James Kirui, Eric O. Freed
doaj +1 more source
NanoLuc luciferase as a quantitative yeast two-hybrid reporter
FEMS Yeast Research, 2021 ABSTRACT The yeast two-hybrid (Y2H) assay is a powerful technique to identify protein-protein interactions. However, the auxotrophic markers that are the most common Y2H reporters take several days to yield data and require subjective assessment of semiquantitative data to identify interactions.
Veronica J, Heintz +2 more
openaire +2 more sources