Results 21 to 30 of about 131,173 (232)

Unraveling Receptor Stoichiometry Using Bret [PDF]

Frontiers in Endocrinology, 2012
The first and arguably most important question that could be asked about the biology of any protein is: does it function alone? Cell surface receptors present special problems for stoichiometric analysis because, being located within lipid bilayers, they are often very hydrophobic, which means that once isolated they can exhibit a strong tendency to ...
Felce, J, Davis, S
openaire   +4 more sources

BRET-based effector membrane translocation assay monitors GPCR-promoted and endocytosis-mediated Gq activation at early endosomes

Proceedings of the National Academy of Sciences of the United States of America, 2021
Significance G protein–coupled receptors (GPCRs) have been proposed to signal in multiple compartments within the cell, a phenomenon that could have important implications for our understanding of receptor pharmacology and disease treatment.
Shane C. Wright, V. Lukasheva, C. Le Gouill, Hiroyuki Kobayashi, B. Breton, S. Mailhot-Larouche, Élodie Blondel-Tepaz, Nichelle Antunes Vieira, C. Costa-Neto, Madeleine Héroux, N. Lambert, Lucas T. Parreiras-e-Silva, M. Bouvier   +12 more
semanticscholar   +1 more source

A synthetic BRET-based optogenetic device for pulsatile transgene expression enabling glucose homeostasis in mice

Nature Communications, 2021
Pulsing cellular dynamics in genetic circuits have been shown to provide critical capabilities to cells in stress response, signaling and development.
Ting Li, Xianjun Chen, Yajie Qian, Jiawei Shao, Xie Li, Shuning Liu, Linyong Zhu, Yuzheng Zhao, Haifeng Ye, Yi Yang   +9 more
semanticscholar   +1 more source

Genetically-encoded BRET probes shed light on ligand bias–induced variable ion selectivity in TRPV1 and P2X5/7

Proceedings of the National Academy of Sciences of the United States of America, 2022
Significance Thanks to innovative BRET-based probes, we shed light on ligand-biased ion selectivity, a difficult-to-study ion channel–related pharmacological concept, by performing the dynamic measurements of Ca2+ and K+ concentrations and ionic strength
Y. Chappe, S. Pierredon, Alexandre Joushomme, Pablo Molle, A. Garenne, Anne Canovi, S. Barbeau, Florence Poulletier De Gannes, A. Hurtier, I. Lagroye, T. Ducret, J. Quignard, V. Compan, Y. Percherancier   +13 more
semanticscholar   +1 more source

Quantitative Profiling of WNT-3A Binding to All Human Frizzled Paralogues in HEK293 Cells by NanoBiT/BRET Assessments

ACS Pharmacology & Translational Science, 2021
The WNT signaling system governs critical processes during embryonic development and tissue homeostasis, and its dysfunction can lead to cancer. Details concerning selectivity and differences in relative binding affinities of 19 mammalian WNTs to the ...
P. Kozielewicz, Rawan Shekhani, Stefanie Moser, Carl-Fredrik Bowin, Janine Wesslowski, G. Davidson, G. Schulte   +6 more
semanticscholar   +1 more source

Frizzled BRET sensors based on bioorthogonal labeling of unnatural amino acids reveal WNT-induced dynamics of the cysteine-rich domain

bioRxiv, 2021
Frizzleds (FZD1-10) comprise a class of G protein-coupled receptors containing an extracellular cysteine-rich domain (CRD) that binds lipoglycoproteins of the Wingless/Int-1 family (WNTs).
Maria Kowalski-Jahn, Hannes Schihada, Ainoleena Turku, T. Huber, T. Sakmar, G. Schulte   +5 more
semanticscholar   +1 more source

Monitoring TRPC7 Conformational Changes by BRET Following GPCR Activation

International Journal of Molecular Sciences, 2022
Transient receptor potential canonical (TRPC) channels are membrane proteins involved in regulating Ca2+ homeostasis, and whose functions are modulated by G protein-coupled receptors (GPCR).
Cécile Pétigny, Audrey-Ann Dumont, Hugo Giguère, Audrey Collette, Brian Holleran, M. Iftinca, C. Altier, É. Besserer-Offroy, M. Auger-Messier, R. Leduc   +9 more
semanticscholar   +1 more source

NanoBRET: The Bright Future of Proximity-Based Assays

Frontiers in Bioengineering and Biotechnology, 2019
Bioluminescence resonance energy transfer (BRET) is a biophysical technique used to monitor proximity within live cells. BRET exploits the naturally occurring phenomenon of dipole-dipole energy transfer from a donor enzyme (luciferase) to an acceptor ...
Natasha C. Dale, Natasha C. Dale, Natasha C. Dale, Elizabeth K. M. Johnstone, Elizabeth K. M. Johnstone, Elizabeth K. M. Johnstone, Carl W. White, Carl W. White, Carl W. White, Kevin D. G. Pfleger, Kevin D. G. Pfleger, Kevin D. G. Pfleger, Kevin D. G. Pfleger   +12 more
doaj   +1 more source

Integration of Nanomaterials and Bioluminescence Resonance Energy Transfer Techniques for Sensing Biomolecules

Biosensors, 2019
Bioluminescence resonance energy transfer (BRET) techniques offer a high degree of sensitivity, reliability and ease of use for their application to sensing biomolecules.
Eugene Hwang, Jisu Song, Jin Zhang
doaj   +1 more source

A Deep Learning Framework for Early Detection of Potential Cardiac Anomalies via Murmur Pattern Analysis in Phonocardiograms

Engineering Proceedings
Heart murmurs, resulting from turbulent blood flow within the cardiac structure, represent some of the initial acoustic manifestations of potential underlying cardiovascular anomalies, such as arrhythmias. This research presents a deep learning framework
Aymane Edder, Fatima-Ezzahraa Ben-Bouazza, Oumaima Manchadi, Youssef Ait Bigane, Djeneba Sangare, Bassma Jioudi   +5 more
doaj   +1 more source