Results 21 to 30 of about 14,160 (163)

BRET biosensor analysis of receptor tyrosine kinase functionality

Frontiers in Endocrinology, 2013
Bioluminescence resonance energy transfer (BRET) is an improved version of earlier resonance energy transfer technologies used for the analysis of biomolecular protein interaction.
Sana eSiddiqui, Wei-na eCong, Caitlin M Daimon, Bronwen eMartin, Stuart eMaudsley   +4 more
doaj   +1 more source

Auto-luminescent genetically-encoded ratiometric indicator for real-time Ca2+ imaging at the single cell level. [PDF]

PLoS ONE, 2010
BACKGROUND: Efficient bioluminescence resonance energy transfer (BRET) from a bioluminescent protein to a fluorescent protein with high fluorescent quantum yield has been utilized to enhance luminescence intensity, allowing single-cell imaging in near ...
Kenta Saito, Noriyuki Hatsugai, Kazuki Horikawa, Kentaro Kobayashi, Toru Matsu-Ura, Katsuhiko Mikoshiba, Takeharu Nagai   +6 more
doaj   +1 more source

CT-to-Toxicity: A Proof-of-Concept AI Pipeline for Automatic Segmentation, Dose Estimation, and Toxicity Prediction in Breast Cancer Radiotherapy

IEEE Access
In breast cancer radiotherapy, clinicians must deliver an adequate tumor dose while protecting surrounding healthy organs. This balance remains difficult: planning is often limited by manual contours, the loss of spatial information, and simplified ...
Manal Chakour El Mezali, Fatima-Ezzahraa Ben-Bouazza, Idriss Tafala, Ilyass Emssaad, Bassma Jioudi   +4 more
doaj   +1 more source

Using nanoBRET and CRISPR/Cas9 to monitor proximity to a genome-edited protein in real-time

Scientific Reports, 2017
Bioluminescence resonance energy transfer (BRET) has been a vital tool for understanding G protein-coupled receptor (GPCR) function. It has been used to investigate GPCR-protein and/or -ligand interactions as well as GPCR oligomerisation.
Carl W. White, Hannah K. Vanyai, Heng B. See, Elizabeth K. M. Johnstone, Kevin D. G. Pfleger   +4 more
doaj   +1 more source

Evolution of BRET biosensors from live cell to tissue-scale in vivo imaging

Frontiers in Endocrinology, 2013
Development of bioluminescence resonance energy transfer [BRET] based genetic sensors for sensing biological functions such as protein-protein interactions [PPIs] in vivo has a special value in measuring such dynamic events at their native environment ...
ABHIJIT eDE, Akshi eJasani, Rohit eArora, Sanjiv Sam Gambhir   +3 more
doaj   +1 more source

A Neuro-Symbolic Clinical Agent for cardiovascular risk stratification: Integrating stacking ensembles with simulation-augmented reasoning

Informatics in Medicine Unlocked
This research presents a Neuro-Symbolic Clinical Agent for cardiovascular risk assessment that integrates ensemble machine learning with tightly coupled counterfactual simulation and targeted guideline retrieval. The framework is trained and validated on
Youssef Ait Bigane, Fatima-Ezzahraa Ben-Bouazza, Aymane Edder, Ilyass Emssaad, Djeneba Sangare, Oumaima Manchadi, Bassma Jioudi   +6 more
doaj   +1 more source

Predicting response to platinum and non-platinum drugs through bioluminescence resonance energy transfer (BRET) based bio-molecular interactions in platinum resistant epithelial ovarian cancer

Translational Oncology, 2021
Therapy induced rewiring of signalling networks often lead to acquirement of platinum-resistance, thereby necessitating the use of non-platinum agents as second-line treatment particularly for epithelial ovarian cancer (EOC).
Aniketh Bishnu, Megha Mehrotra, Ajit Dhadve, Shalini Dimri, Abhijit De, Pritha Ray   +5 more
doaj   +1 more source

DeepPatchNet: A deep learning model for enhanced screening and diagnosis of oral cancer

Informatics in Medicine Unlocked
Oral cancer remains a serious global health challenge, significantly affecting patient survival and quality of life. While convolutional neural networks (CNNs) have historically dominated image classification tasks, recent advances suggest that ...
Idriss Tafala, Fatima-Ezzahraa Ben-Bouazza, Aymane Edder, Oumaima Manchadi, Bassma Jioudi   +4 more
doaj   +1 more source

Setting up a Bioluminescence Resonance Energy Transfer high throughput screening assay to search for protein/protein interaction inhibitors in mammalian cells.

Frontiers in Endocrinology, 2012
Each step of the cell life and its response or adaptation to its environment are mediated by a network of protein/protein interactions termed interactome.
Cyril eCouturier, Benoit eDeprez
doaj   +1 more source

Leveraging multilingual RAG for breast cancer RCPs: AI-driven speech transcription and compliance in Darija-French clinical discussions

Computer Methods and Programs in Biomedicine Update
The integration of artificial intelligence (AI) into clinical decision-making has introduced new opportunities for automating and enhancing medical documentation, particularly in oncology, where multidisciplinary meetings are central to treatment ...
Ilyass Emssaad, Fatima-Ezzahraa Ben-Bouazza, Idriss Tafala, Manal Chakour El Mezali, Bassma Jioudi   +4 more
doaj   +1 more source