Results 41 to 50 of about 131,173 (232)

Auto-luminescent genetically-encoded ratiometric indicator for real-time Ca2+ imaging at the single cell level. [PDF]

PLoS ONE, 2010
BACKGROUND: Efficient bioluminescence resonance energy transfer (BRET) from a bioluminescent protein to a fluorescent protein with high fluorescent quantum yield has been utilized to enhance luminescence intensity, allowing single-cell imaging in near ...
Kenta Saito, Noriyuki Hatsugai, Kazuki Horikawa, Kentaro Kobayashi, Toru Matsu-Ura, Katsuhiko Mikoshiba, Takeharu Nagai   +6 more
doaj   +1 more source

Bioluminescence Resonance Energy Transfer (BRET)-mediated Protein Release from Self-Illuminating Photoresponsive Biomaterials

Journal of the American Chemical Society
Phototriggered release of various cargos, including soluble protein factors and small molecules, has the potential to correct aberrant biological events by offering spatiotemporal control over local therapeutic levels. However, the poor penetration depth
Teresa L. Rapp, Irina Kopyeva, Abhinav Adhikari, Cole A. DeForest   +3 more
semanticscholar   +1 more source

CT-to-Toxicity: A Proof-of-Concept AI Pipeline for Automatic Segmentation, Dose Estimation, and Toxicity Prediction in Breast Cancer Radiotherapy

IEEE Access
In breast cancer radiotherapy, clinicians must deliver an adequate tumor dose while protecting surrounding healthy organs. This balance remains difficult: planning is often limited by manual contours, the loss of spatial information, and simplified ...
Manal Chakour El Mezali, Fatima-Ezzahraa Ben-Bouazza, Idriss Tafala, Ilyass Emssaad, Bassma Jioudi   +4 more
doaj   +1 more source

Using nanoBRET and CRISPR/Cas9 to monitor proximity to a genome-edited protein in real-time

Scientific Reports, 2017
Bioluminescence resonance energy transfer (BRET) has been a vital tool for understanding G protein-coupled receptor (GPCR) function. It has been used to investigate GPCR-protein and/or -ligand interactions as well as GPCR oligomerisation.
Carl W. White, Hannah K. Vanyai, Heng B. See, Elizabeth K. M. Johnstone, Kevin D. G. Pfleger   +4 more
doaj   +1 more source

Evolution of BRET biosensors from live cell to tissue-scale in vivo imaging

Frontiers in Endocrinology, 2013
Development of bioluminescence resonance energy transfer [BRET] based genetic sensors for sensing biological functions such as protein-protein interactions [PPIs] in vivo has a special value in measuring such dynamic events at their native environment ...
ABHIJIT eDE, Akshi eJasani, Rohit eArora, Sanjiv Sam Gambhir   +3 more
doaj   +1 more source

Do Brain Extracellular Fluid Concentrations Predict PET‐Inferred Receptor Occupancy in Human CNS? A Mechanistic Model‐Based Analysis of Dopamine D2 Receptor Ligands

Clinical Pharmacology &Therapeutics, EarlyView.
A mechanistic CNS PBPK model framework was applied to eight antipsychotic D2 receptor ligands, comparing brain extracellular fluid (brainECF) and brain cell membrane (brainM) as biophase for predicting human receptor occupancy compared against PET neuroimaging data. BrainM concentrations showed improved agreement with PET‐derived receptor occupancy for
Divakar Budda, J.G. Coen van Hasselt, Elizabeth C.M. de Lange   +2 more
wiley   +1 more source

Class-Wide Analysis of Frizzled-Dishevelled Interactions Using BRET Biosensors Reveals Functional Differences among Receptor Paralogs

ACS Sensors
Wingless/Int-1 (WNT) signaling is mediated by WNT binding to 10 Frizzleds (FZD1–10), which propagate the signal inside the cell by interacting with different transducers, most prominently the phosphoprotein Dishevelled (DVL).
Lukas Grätz, J. Voss, Gunnar Schulte
semanticscholar   +1 more source

In vivo imaging of long-term accumulation of cancer-derived exosomes using a BRET-based reporter

Scientific Reports, 2020
Monitoring of exosome dynamics in living organisms is essential to demonstrate the real functions of cancer-derived exosomes. Currently, these have been elucidated in vitro or under non-physiological conditions in vivo in most cases.
T. Hikita, Mamiko Miyata, Risayo Watanabe, Chitose Oneyama   +3 more
semanticscholar   +1 more source

A Neuro-Symbolic Clinical Agent for cardiovascular risk stratification: Integrating stacking ensembles with simulation-augmented reasoning

Informatics in Medicine Unlocked
This research presents a Neuro-Symbolic Clinical Agent for cardiovascular risk assessment that integrates ensemble machine learning with tightly coupled counterfactual simulation and targeted guideline retrieval. The framework is trained and validated on
Youssef Ait Bigane, Fatima-Ezzahraa Ben-Bouazza, Aymane Edder, Ilyass Emssaad, Djeneba Sangare, Oumaima Manchadi, Bassma Jioudi   +6 more
doaj   +1 more source

Predicting response to platinum and non-platinum drugs through bioluminescence resonance energy transfer (BRET) based bio-molecular interactions in platinum resistant epithelial ovarian cancer

Translational Oncology, 2021
Therapy induced rewiring of signalling networks often lead to acquirement of platinum-resistance, thereby necessitating the use of non-platinum agents as second-line treatment particularly for epithelial ovarian cancer (EOC).
Aniketh Bishnu, Megha Mehrotra, Ajit Dhadve, Shalini Dimri, Abhijit De, Pritha Ray   +5 more
doaj   +1 more source