Results 61 to 70 of about 131,173 (232)
Gender &History, EarlyView.ABSTRACT This article considers travel writings by metropolitan men in Republican China about Shanxi and western Inner Mongolia as a case study to further explore the transformations and continuities of Chinese masculinities. Drawing upon a range of popular travel narratives, it shows that so‐called “Worn‐Out Shoes (poxie)” – women perceived as ...
Amanda Zhang
wiley +1 more source
Bio-Protocol, 2019 Detecting protein-protein interactions by co-immunoprecipitation provided a major advancement in the immunology research field. In the G-protein-coupled receptors (GPCRs) research field, colocalization and co-immunoprecipitation were used to detect ...
Irene Reyes-Resina +3 more
doaj +1 more source
American Journal of Agricultural Economics, EarlyView.Abstract A longstanding puzzle in the African land rental market literature is the often‐observed discrepancy between the number of tenants (renters‐in) and the much smaller number of landlords (renters‐out) in survey data. If this discrepancy derives from systematic biases in survey data responses on rental market participation, then the existing body
Gashaw T. Abate +3 more
wiley +1 more source
Analytica Chimica Acta: X, 2020 Bioluminescence Resonance Energy Transfer (BRET) is widely applied to study protein-protein interactions, as well as increasingly to monitor both ligand binding and molecular rearrangements.
F. Weihs +3 more
semanticscholar +1 more source
Regulator of G protein signaling 2 (RGS2) and RGS4 form distinct G protein-dependent complexes with protease activated-receptor 1 (PAR1) in live cells. [PDF]
PLoS ONE, 2014 Protease-activated receptor 1 (PAR1) is a G-protein coupled receptor (GPCR) that is activated by natural proteases to regulate many physiological actions.
Sungho Ghil +2 more
doaj +1 more source
STAR Protocols, 2022 Summary: The Kinetic Intra-Cellular Assay (KICA) is a recombinant cell-based technique that utilizes NanoBRET technology. KICA enables the measurement of intracellular binding kinetics.
Charles S. Lay +5 more
doaj +1 more source
Compartmentalisation in cAMP signalling: A phase separation perspective
British Journal of Pharmacology, EarlyView.Cells rely on precise spatiotemporal control of signalling pathways to ensure functional specificity. The compartmentalisation of cyclic AMP (cAMP) and protein kinase A (PKA) signalling enables distinct cellular responses within a crowded cytoplasmic space.
Milda Folkmanaite, Manuela Zaccolo
wiley +1 more source
British Journal of Pharmacology, EarlyView.Background and Purpose Migraine is a neurovascular disorder largely mediated by calcitonin gene‐related peptide (CGRP). This study explores the role of the nociceptin/orphanin FQ (N/OFQ)–N/OFQ receptor (NOP) system in CGRP‐induced periorbital mechanical allodynia (PMA) in mice.
Chiara Sturaro +14 more
wiley +1 more source
BRET-Based Self-Cleaving Biosensors for SARS-CoV-2 3CLpro Inhibitor Discovery
bioRxiv, 2021 The virus proteases 3CLpro are validated drug targets for developing antivirals to treat coronavirus diseases, such as COVID-19. However, the development of 3CLpro inhibitors relies heavily on BSL-3 laboratories. ABSTRACT The 3C-like protease (3CLpro) of
Ningke Hou +4 more
semanticscholar +1 more source
Applied Sciences, 2020 DNA-protein conjugates are useful molecules for construction of biosensors. Herein, we report the development of an enzymatically-conjugated DNA aptamer–protein hybrid molecule for use as a bioluminescence resonance energy transfer (BRET)-based biosensor.
Masayasu Mie +3 more
doaj +1 more source