Results 61 to 70 of about 39,288 (183)

NanoBRET in C. elegans illuminates functional receptor interactions in real time

open access: yesBMC Molecular and Cell Biology, 2022
Background Protein-protein interactions form the basis of every organism and thus, investigating their dynamics, intracellular protein localization, trafficking and interactions of distinct proteins such as receptors and their ligand-binding are of ...
Victoria Elisabeth Groß   +4 more
doaj   +1 more source

The Allais Paradox and Upside/Downside Risk Preference: An Experimental Analysis With Probability Weighting and Chernoff Faces

open access: yesSouthern Economic Journal, EarlyView.
ABSTRACT The Allais Paradox is often attributed to the strong attraction of a certain option that avoids a downside risk, i.e., a small chance of a low payoff. In contrast, there is clear evidence from laboratory and field data that people are attracted to the upside risk of obtaining a relatively high payoff.
Irene Comeig, Charles Holt
wiley   +1 more source

Diagnostic antibodies at an inflection point: From discovery platforms to design‐oriented engineering

open access: yesVIEW, EarlyView.
This study systematically organizes the evolution of diagnostic antibodies from discovery platforms to design‐oriented engineering and analyzes the strengths and limitations of each approach. Meanwhile, it provides a critical, literature‐driven synthesis of current challenges, proposing integrated strategies for assay‐aware and function‐driven antibody
Qitao Song   +14 more
wiley   +1 more source

Luciferase-induced photoreductive uncaging of small-molecule effectors

open access: yesNature Communications, 2018
Bioluminescence resonance energy transfer (BRET) has been mostly employed in imaging applications. Here the authors use BRET to activate a ruthenium-based photocatalyst and perform a bioorthogonal chemical reaction, which can be used to uncage small ...
Eric Lindberg   +3 more
doaj   +1 more source

Patient‐reported outcomes, postoperative pain and pain relief after day‐case surgery (POPPY): acute pain and recovery during the first 7 days

open access: yesAnaesthesia, EarlyView.
Summary Introduction The majority of surgical cases in the UK occur in a day‐case setting. Published short‐term follow‐up studies after day‐case surgery are lacking and patterns of recovery including pain and patient reported outcome measures, are largely unknown. Methods Data from the Patient‐reported Outcomes, Postoperative Pain and pain relief after
Matthew Everson   +2177 more
wiley   +1 more source

Application of quantum dot nanoparticles for potential non-invasive bio-imaging of mammalian spermatozoa

open access: yesJournal of Nanobiotechnology, 2012
Background Various obstacles are encountered by mammalian spermatozoa during their journey through the female genital tract, and only few or none will reach the site of fertilization.
Feugang Jean M   +6 more
doaj   +1 more source

Compartmentalisation in cAMP signalling: A phase separation perspective

open access: yesBritish Journal of Pharmacology, EarlyView.
Cells rely on precise spatiotemporal control of signalling pathways to ensure functional specificity. The compartmentalisation of cyclic AMP (cAMP) and protein kinase A (PKA) signalling enables distinct cellular responses within a crowded cytoplasmic space.
Milda Folkmanaite, Manuela Zaccolo
wiley   +1 more source

Activation of peripheral NOP receptors reduces periorbital mechanical allodynia evoked by CGRP in mice

open access: yesBritish Journal of Pharmacology, EarlyView.
Background and Purpose Migraine is a neurovascular disorder largely mediated by calcitonin gene‐related peptide (CGRP). This study explores the role of the nociceptin/orphanin FQ (N/OFQ)–N/OFQ receptor (NOP) system in CGRP‐induced periorbital mechanical allodynia (PMA) in mice.
Chiara Sturaro   +14 more
wiley   +1 more source

Enhanced BRET technology for the monitoring of agonist-induced and agonist-independent interactions between GPCRs and β-arrestins

open access: yesFrontiers in Endocrinology, 2011
The bioluminescence resonance energy transfer (BRET) technique has become extremely valuable for the real-time monitoring of protein-protein interactions in live cells.
Martina eKocan   +5 more
doaj   +1 more source

The potential for biased signalling in the P2Y receptor family of GPCRs

open access: yesBritish Journal of Pharmacology, EarlyView.
The purinergic receptor family is primarily activated by nucleotides, and contains members of both the G protein coupled‐receptor (GPCR) superfamily (P1 and P2Y) and ligand‐gated ion channels (P2X). The P2Y receptors are widely expressed in the human body, and given the ubiquitous nature of nucleotides, purinergic signalling is involved with a plethora
Claudia M. Sisk   +2 more
wiley   +1 more source

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