Integration of Nanomaterials and Bioluminescence Resonance Energy Transfer Techniques for Sensing Biomolecules [PDF]
Biosensors, 2019 Bioluminescence resonance energy transfer (BRET) techniques offer a high degree of sensitivity, reliability and ease of use for their application to sensing biomolecules.
Eugene Hwang, Jisu Song, Jin Zhang
doaj +4 more sources
Comparison of static and microfluidic protease assays using modified bioluminescence resonance energy transfer chemistry. [PDF]
PLoS ONE, 2014 BackgroundFluorescence and bioluminescence resonance energy transfer (F/BRET) are two forms of Förster resonance energy transfer, which can be used for optical transduction of biosensors.
Nan Wu +4 more
doaj +4 more sources
Ratiometric Detection of Zn2+ Using DNAzyme-Based Bioluminescence Resonance Energy Transfer Sensors [PDF]
Chemistry, 2023 While fluorescent sensors have been developed for monitoring metal ions in health and diseases, they are limited by the requirement of an excitation light source that can lead to photobleaching and a high autofluorescence background.
Yuting Wu +10 more
doaj +2 more sources
Enhanced brightness of bacterial luciferase by bioluminescence resonance energy transfer [PDF]
Scientific Reports, 2021 Using the lux operon (luxCDABE) of bacterial bioluminescence system as an autonomous luminous reporter has been demonstrated in bacteria, plant and mammalian cells.
Tomomi Kaku +4 more
doaj +2 more sources
Application of bioluminescence resonance energy transfer-based cell tracking approach in bone tissue engineering [PDF]
Journal of Tissue Engineering, 2021 Bioluminescent imaging (BLI) has emerged as a popular in vivo tracking modality in bone regeneration studies stemming from its clear advantages: non-invasive, real-time, and inexpensive. We recently adopted bioluminescence resonance energy transfer (BRET)
Lufei Wang +13 more
doaj +2 more sources
Image Processing for Bioluminescence Resonance Energy Transfer Measurement—BRET-Analyzer [PDF]
Frontiers in Computational Neuroscience, 2018 A growing number of tools now allow live recordings of various signaling pathways and protein-protein interaction dynamics in time and space by ratiometric measurements, such as Bioluminescence Resonance Energy Transfer (BRET) Imaging.
Yan Chastagnier +11 more
doaj +2 more sources
Creation of different bioluminescence resonance energy transfer based biosensors with high affinity to VEGF. [PDF]
PLoS ONE, 2020 In age-related macular degeneration (AMD) or diabetic retinopathy (DR), hypoxia and inflammatory processes lead to an upregulation of the vascular endothelial growth factor (VEGF) expression and thereby to pathological neovascularisation with incorrectly
Constanze Stumpf +3 more
doaj +2 more sources
Application of bioluminescence resonance energy transfer assays in primary mouse neuronal cultures [PDF]
STAR ProtocolsSummary: Bioluminescence resonance energy transfer (BRET) is widely employed for real-time monitoring of G protein-coupled receptor activity, interactions, and trafficking in heterologous cell lines, yet its use in neuronal systems remains limited. Here,
Hanh T.M. Hoang +2 more
doaj +2 more sources
Bioluminescence Resonance Energy Transfer (BRET) Assay for Determination of Molecular Interactions in Living Cells [PDF]
Bio-Protocol, 2017 The bioluminescence resonance energy transfer (BRET) assay can be used as an indicator of molecular approximation and/or interaction. A significant resonance energy transfer signal is generated when the acceptor, having the appropriate spectral overlap ...
Kaleeckal Harikumar +5 more
doaj +2 more sources
A Bioluminescence Resonance Energy Transfer-Based Approach for Determining Antibody-Receptor Occupancy In Vivo [PDF]
iScience, 2019 Summary: Elucidating receptor occupancy (RO) of monoclonal antibodies (mAbs) is a crucial step in characterizing the therapeutic efficacy of mAbs. However, the in vivo assessment of RO, particularly within peripheral tissues, is greatly limited by ...
Yu Tang +3 more
doaj +2 more sources