Protocol to measure and analyze protein interactions in mammalian cells using bioluminescence resonance energy transfer [PDF]
STAR ProtocolsSummary: Bioluminescence resonance energy transfer (BRET) allows to quantitate protein interactions in intact cells. Here, we present a protocol for measuring BRET due to transient interactions of oncogenic K-RasG12V in plasma membrane nanoclusters of ...
Carla Jane Duval +3 more
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Genetically Encoded RNA-Based Bioluminescence Resonance Energy Transfer (BRET) Sensors. [PDF]
ACS Sens, 2023 Abstract RNA-based nanostructures and molecular devices have become popular for developing biosensors and genetic regulators. These programmable RNA nanodevices can be genetically encoded and modularly engineered to detect various cellular targets and then induce output signals, most often a fluorescence readout ...
Mi L +7 more
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Multiplexing of multicolor bioluminescence resonance energy transfer. [PDF]
Biophys J, 2010 Bioluminescence resonance energy transfer (BRET) is increasingly being used to monitor protein-protein interactions and cellular events in cells. However, the ability to monitor multiple events simultaneously is limited by the spectral properties of the ...
Breton B +5 more
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Resonance Energy Transfer Molecular Imaging Application in Biomedicine
Journal of Isotopes, 2016 Resonance energy transfer molecular imaging (RETI) can markedly improve signal intensity and tissue penetrating capacity of optical imaging, and have huge potential application in the deep-tissue optical imaging in vivo.
NIE Da-hong1,2;TANG Gang-hua1,3
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Biophysica, 2022 Bioluminescence resonance energy transfer (BRET) seems to be a promising biophysical technique to study protein–protein interactions within living cells due to a very specific reaction of bioluminescence that essentially decreases the background of other
Victor Marchenkov +4 more
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Bio-Protocol, 2019 Detecting protein-protein interactions by co-immunoprecipitation provided a major advancement in the immunology research field. In the G-protein-coupled receptors (GPCRs) research field, colocalization and co-immunoprecipitation were used to detect ...
Irene Reyes-Resina +3 more
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Bioluminescence Resonance Energy Transfer System for Measuring Dynamic Protein-Protein Interactions in Bacteria [PDF]
mBio, 2014 Protein-protein interactions are important for virtually every biological process, and a number of elegant approaches have been designed to detect and evaluate such interactions.
Boyu Cui +7 more
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In-cell bioluminescence resonance energy transfer (BRET)-based assay uncovers ceritinib and CA-074 as SARS-CoV-2 papain-like protease inhibitors [PDF]
Journal of Enzyme Inhibition and Medicinal ChemistryPapain-like protease (PLpro) is an attractive anti-coronavirus target. The development of PLpro inhibitors, however, is hampered by the limitations of the existing PLpro assay and the scarcity of validated active compounds.
Mei Li +8 more
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Analysis of RXR/THR and RXR/PPARG2 heterodimerization by bioluminescence resonance energy transfer (BRET). [PDF]
PLoS ONE, 2013 BACKGROUND: Nuclear receptors (NR) regulate transcription of genes involved in many biological processes such as development, cell proliferation, differentiation and cell death.
Miquel Mulero +4 more
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Analysis of in vitro SUMOylation using bioluminescence resonance energy transfer (BRET)
Biochemical and Biophysical Research Communications, 2009 We demonstrated in vitro small ubiquitin-like modifier (SUMO)-mediated modification (SUMOylation) of RanGTPase activating protein-1 (RdnGAP1) by using bioluminescence resonance energy transfer (BRET) for studying protein interactions. Renilla luciferase (
Park, Sun-Young +5 more
core +4 more sources