Results 11 to 20 of about 7,702 (129)

Protocol to measure and analyze protein interactions in mammalian cells using bioluminescence resonance energy transfer [PDF]

STAR Protocols
Summary: Bioluminescence resonance energy transfer (BRET) allows to quantitate protein interactions in intact cells. Here, we present a protocol for measuring BRET due to transient interactions of oncogenic K-RasG12V in plasma membrane nanoclusters of ...
Carla Jane Duval, Candy Laura Steffen, Karolina Pavic, Daniel Kwaku Abankwa   +3 more
doaj   +2 more sources

Bioluminescence Resonance Energy Transfer System for Measuring Dynamic Protein-Protein Interactions in Bacteria [PDF]

mBio, 2014
Protein-protein interactions are important for virtually every biological process, and a number of elegant approaches have been designed to detect and evaluate such interactions.
Boyu Cui, Yao Wang, Yunhong Song, Tietao Wang, Changfu Li, Yahong Wei, Zhao-Qing Luo, Xihui Shen   +7 more
doaj   +2 more sources

Identification of Heteroreceptors Complexes and Signal Transduction Events Using Bioluminescence Resonance Energy Transfer (BRET)

Bio-Protocol, 2019
Detecting protein-protein interactions by co-immunoprecipitation provided a major advancement in the immunology research field. In the G-protein-coupled receptors (GPCRs) research field, colocalization and co-immunoprecipitation were used to detect ...
Irene Reyes-Resina, Jasmina Jiménez, Gemma Navarro, Rafael Franco   +3 more
doaj   +2 more sources

Analysis of RXR/THR and RXR/PPARG2 heterodimerization by bioluminescence resonance energy transfer (BRET). [PDF]

PLoS ONE, 2013
BACKGROUND: Nuclear receptors (NR) regulate transcription of genes involved in many biological processes such as development, cell proliferation, differentiation and cell death.
Miquel Mulero, Julie Perroy, Carole Federici, Gérard Cabello, Vincent Ollendorff   +4 more
doaj   +2 more sources

In-cell bioluminescence resonance energy transfer (BRET)-based assay uncovers ceritinib and CA-074 as SARS-CoV-2 papain-like protease inhibitors [PDF]

Journal of Enzyme Inhibition and Medicinal Chemistry
Papain-like protease (PLpro) is an attractive anti-coronavirus target. The development of PLpro inhibitors, however, is hampered by the limitations of the existing PLpro assay and the scarcity of validated active compounds.
Mei Li, Zhu-Chun Bei, Yongtian Yuan, Baogang Wang, Dongna Zhang, Likun Xu, Liangliang Zhao, Qin Xu, Yabin Song   +8 more
doaj   +2 more sources

Bioluminescence Resonance Energy Transfer as a Method to Study Protein-Protein Interactions: Application to G Protein Coupled Receptor Biology [PDF]

Molecules, 2019
The bioluminescence resonance energy transfer (BRET) approach involves resonance energy transfer between a light-emitting enzyme and fluorescent acceptors.
Chayma El Khamlichi, Flora Reverchon-Assadi, Nadège Hervouet-Coste, Lauren Blot, Eric Reiter, Séverine Morisset-Lopez   +5 more
doaj   +2 more sources

Genetically Encoded RNA-Based Bioluminescence Resonance Energy Transfer (BRET) Sensors. [PDF]

ACS Sens, 2023
Mi L, Yu Q, Karunanayake Mudiyanselage APKK, Wu R, Sun Z, Zheng R, Ren K, You M.   +7 more
europepmc   +2 more sources

Multiresolution Imaging Using Bioluminescence Resonance Energy Transfer Identifies Distinct Biodistribution Profiles of Extracellular Vesicles and Exomeres with Redirected Tropism. [PDF]

Adv Sci (Weinh), 2020
Wu AY, Sung YC, Chen YJ, Chou ST, Guo V, Chien JC, Ko JJ, Yang AL, Huang HC, Chuang JC, Wu S, Ho MR, Ericsson M, Lin WW, Cheung CHY, Juan HF, Ueda K, Chen Y, Lai CP.   +18 more
europepmc   +2 more sources

Developments in FRET- and BRET-Based Biosensors

Micromachines, 2022
Resonance energy transfer technologies have achieved great success in the field of analysis. Particularly, fluorescence resonance energy transfer (FRET) and bioluminescence resonance energy transfer (BRET) provide strategies to design tools for sensing ...
Yuexin Wu, Tianyu Jiang
doaj   +1 more source

In Vivo Analysis of Protein-Protein Interactions with Bioluminescence Resonance Energy Transfer (BRET): Progress and Prospects. [PDF]

Int J Mol Sci, 2016
Sun S, Yang X, Wang Y, Shen X.
europepmc   +2 more sources