Genetically Encoded RNA-Based Bioluminescence Resonance Energy Transfer (BRET) Sensors. [PDF]
ACS Sens, 2023 Abstract RNA-based nanostructures and molecular devices have become popular for developing biosensors and genetic regulators. These programmable RNA nanodevices can be genetically encoded and modularly engineered to detect various cellular targets and then induce output signals, most often a fluorescence readout ...
Mi L +7 more
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Enhanced Molecular Tension Sensor Based on Bioluminescence Resonance Energy Transfer (BRET). [PDF]
ACS Sens, 2020 Molecular tension sensors measure piconewton forces experienced by individual proteins in the context of the cellular microenvironment. Current genetically encoded tension sensors use FRET to report on extension of a deformable peptide encoded in a cellular protein of interest.
Aird EJ +3 more
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Protocol to measure and analyze protein interactions in mammalian cells using bioluminescence resonance energy transfer [PDF]
STAR ProtocolsSummary: Bioluminescence resonance energy transfer (BRET) allows to quantitate protein interactions in intact cells. Here, we present a protocol for measuring BRET due to transient interactions of oncogenic K-RasG12V in plasma membrane nanoclusters of ...
Carla Jane Duval +3 more
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Bioluminescence Resonance Energy Transfer System for Measuring Dynamic Protein-Protein Interactions in Bacteria [PDF]
mBio, 2014 Protein-protein interactions are important for virtually every biological process, and a number of elegant approaches have been designed to detect and evaluate such interactions.
Boyu Cui +7 more
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Bio-Protocol, 2019 Detecting protein-protein interactions by co-immunoprecipitation provided a major advancement in the immunology research field. In the G-protein-coupled receptors (GPCRs) research field, colocalization and co-immunoprecipitation were used to detect ...
Irene Reyes-Resina +3 more
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Analysis of RXR/THR and RXR/PPARG2 heterodimerization by bioluminescence resonance energy transfer (BRET). [PDF]
PLoS ONE, 2013 BACKGROUND: Nuclear receptors (NR) regulate transcription of genes involved in many biological processes such as development, cell proliferation, differentiation and cell death.
Miquel Mulero +4 more
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In-cell bioluminescence resonance energy transfer (BRET)-based assay uncovers ceritinib and CA-074 as SARS-CoV-2 papain-like protease inhibitors [PDF]
Journal of Enzyme Inhibition and Medicinal ChemistryPapain-like protease (PLpro) is an attractive anti-coronavirus target. The development of PLpro inhibitors, however, is hampered by the limitations of the existing PLpro assay and the scarcity of validated active compounds.
Mei Li +8 more
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Bioluminescence Resonance Energy Transfer as a Method to Study Protein-Protein Interactions: Application to G Protein Coupled Receptor Biology [PDF]
Molecules, 2019 The bioluminescence resonance energy transfer (BRET) approach involves resonance energy transfer between a light-emitting enzyme and fluorescent acceptors.
Chayma El Khamlichi +5 more
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Development of a Human Estrogen Receptor Dimerization Assay for the Estrogenic Endocrine-Disrupting Chemicals Using Bioluminescence Resonance Energy Transfer. [PDF]
Int J Environ Res Public Health, 2021 Kim HM +5 more
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Self-illumination of Carbon Dots by Bioluminescence Resonance Energy Transfer. [PDF]
Sci Rep, 2019 AbstractCarbon-dots (CDs), the emerging fluorescent nanoparticles, show special multicolor properties, chemical stability, and biocompatibility, and are considered as the new and advanced imaging probe in replacement of molecular fluorophores and semiconductor quantum dots.
Song J, Zhang J.
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