Results 41 to 50 of about 7,702 (129)

Protocol for measuring BRAF autoinhibition in live cells using a proximity-based NanoBRET assay

STAR Protocols, 2023
Summary: BRAF is frequently activated via mutation in human cancer and the RASopathy syndromes; however, for BRAF activation to occur, autoinhibitory interactions between the regulatory and catalytic domains must be relieved. Here, we present a proximity-
Russell Spencer-Smith, Deborah K. Morrison   +1 more
doaj   +1 more source

Asymmetrically Coordinated Dual‐Atom Manganese Contrast Agents Enabling High‐Efficiency T1 Enhanced MRI for Precise Tumor Visualization

Advanced Science, EarlyView.
A novel dual‐atom manganese MRI contrast agent (Mn‐BN) with a unique asymmetric coordination geometry provided a promising platform for sensitive and specific tumor imaging. In mice model, Mn‐BN nanoparticles precisely functioned as both a primary tumor‐specific enhancer in subcutaneous models and a hepatocyte‐specific agent in liver metastases models ...
Ding Yang, Qing‐Yang Li, Jianli Liang, Gengyou Li, Nan Sun, Qianqian Song, Haitao Zhu, Xiaoting Li, Zechuan Li, Jiankai Dong, Weisheng Guo, Yafang Xiao, Chun‐Sing Lee, Ying‐Shi Sun   +13 more
wiley   +1 more source

Corrigendum

International Journal of Nanomedicine, 2012
Wu Q, Chu M. Self-illuminating quantum dots for highly sensitive in vivo real-time luminescent mapping of sentinel lymph nodes. Int J Nanomedicine. 2012;7:3433–3443.The following sentence on page 3433 was incorrect in the published paper:& ...
Wu Q, Chu M
doaj  

Bioluminescence-Based Energy Transfer Using Semiconductor Quantum Dots as Acceptors

Sensors, 2020
Bioluminescence resonance energy transfer (BRET) is the non-radiative transfer of energy from a bioluminescent protein donor to a fluorophore acceptor. It shares all the formalism of Förster resonance energy transfer (FRET) but differs in one key aspect:
Anirban Samanta, Igor L. Medintz
doaj   +1 more source

Mitochondrial Carrier SLC25A13 Drives Ferroptosis Resistance and Immune Evasion via a STAT3–IFI6 Circuit in Breast Cancer

Advanced Science, EarlyView.
SLC25A13 is identified as an immunometabolic driver of triple‐negative breast cancer that sustains ferroptosis resistance and immune evasion through a STAT3–IFI6 circuit. Pharmacologic degradation of SLC25A13 restores ferroptosis sensitivity and enhances anti‐PD‐1 efficacy, highlighting a strategy to convert immune‐cold tumors into immunotherapy ...
Yingze Zhu, Yuhan Tang, Yingcui Chen, Zhuoqi Zhang, Yige Lu, Xinyue Wang, Qiyuan Shi, Wenhui Zhao, Hui Pang   +8 more
wiley   +1 more source

Surfaceome Reprogramming of Stemsomes Promotes Lung Cancer Targeting via Potentiated Receptor–Ligand Interactions

Advanced Science, EarlyView.
This study presents a surfaceome‐reprogramming strategy for mutation‐independent lung cancer therapy by repurposing dexamethasone to prime mesenchymal stem cell‐derived nanovesicles. The engineered vesicles leverage multi‐valent interactions mediated by upregulated adhesion proteins, EPHA2, and NOTCH3.
Geunhye Kim, In Ah Kwon, Bo Seop Jeong, Hyo Seon Kim, Joo‐Hwan Park, Sung Jean Park, Sang‐Rae Lee, Youngki Lee, Dongwoo Khang   +8 more
wiley   +1 more source

tracerDB: a crowdsourced fluorescent tracer database for target engagement analysis

Nature Communications
Investigating ligand-protein complexes is essential in the areas of chemical biology and drug discovery. However, detailed information on key reagents such as fluorescent tracers and associated data for the development of widely used bioluminescence ...
Johannes Dopfer, James D. Vasta, Susanne Müller, Stefan Knapp, Matthew B. Robers, Martin P. Schwalm   +5 more
doaj   +1 more source

Single‐Cell Profiling Identifies SLC2A5‐Mediated Fructose Metabolism as a Vulnerability in Primary CNS Lymphoma

Advanced Science, EarlyView.
Glucose deprivation in the primary CNS lymphoma (PCNSL) tumor microenvironment drives SLC2A5 (encoding GLUT5)‐dependent fructose metabolism in tumor cells, while hypoxia induces HIF‐mediated SLC2A5 expression in tumor‐supportive macrophages, revealing SLC2A5‐driven fructose utilization as a shared and targetable metabolic vulnerability across malignant
Qiaoli Wu, Qianru Zhang, Wenqiang Yan, Lu Sun, Yiran Cui, Bolin Liu, Guoqing Han, Feng Zhang, Ke Pu, Mingchao Zhang, Xiaobing Zhang, Qingguo Li, Gang An, Yuxuan Liu   +13 more
wiley   +1 more source

Analysis of Protease Activity Using Quantum Dots and Resonance Energy Transfer

Theranostics, 2012
This review demonstrates the detection of protease activity based on the energy transfer of quantum dots (QDs). By incorporation of varying protease substrates into designed QD probes both in fluorescence resonance energy transfer (FRET) and ...
Gae Baik Kim, Young-Pil Kim
doaj  

Receptor-G Protein Interaction Studied by Bioluminescence Resonance Energy Transfer: Lessons From Protease-Activated Receptor 1

Frontiers in Endocrinology, 2012
Since its development, the bioluminescence resonance energy transfer (BRET) approach has been extensively applied to study G protein-coupled receptors (GPCRs) in real time and in live cells.
Mohammed Akli eAYOUB, Mohammed Akli eAYOUB, Mohammed Akli eAYOUB, Abdulrahman eAl-Senaidy, Jean-Philippe ePin, Jean-Philippe ePin   +5 more
doaj   +1 more source