Results 41 to 50 of about 8,042,386 (212)

Luciferase-induced photoreductive uncaging of small-molecule effectors

Nature Communications, 2018
Bioluminescence resonance energy transfer (BRET) has been mostly employed in imaging applications. Here the authors use BRET to activate a ruthenium-based photocatalyst and perform a bioorthogonal chemical reaction, which can be used to uncage small ...
Eric Lindberg, Simona Angerani, Marcello Anzola, Nicolas Winssinger   +3 more
doaj   +1 more source

Biophysical detection of diversity and bias in GPCR function

Frontiers in Endocrinology, 2014
G protein-coupled receptors (GPCRs) function in complexes with a range of molecules and proteins including ligands, G proteins, arrestins, ubiquitin and other receptors.
Werner eJaeger, Stephen eArmstrong, Stephen eHill, Kevin D G Pfleger, Kevin D G Pfleger   +4 more
doaj   +1 more source

Monitoring G protein-coupled receptor and β-arrestin trafficking in live cells using enhanced bystander BRET

Nature Communications, 2016
Cellular signaling processes often involve trafficking of receptors and other proteins between subcellular compartments. Here the authors demonstrate a method based on the concept of Enhanced bystander Bioluminescence Resonance Energy Transfer (EbBRET ...
Yoon Namkung, Christian Le Gouill, Viktoria Lukashova, Hiroyuki Kobayashi, Mireille Hogue, Etienne Khoury, Mideum Song, Michel Bouvier, Stéphane A. Laporte   +8 more
doaj   +1 more source

Agonist and antagonist TRUPATH assays for G protein-coupled receptors

STAR Protocols, 2022
Summary: TRUPATH is a bioluminescence resonance energy transfer-based platform for quantifying G protein-coupled receptor activity via dissociation of heterotrimeric G protein biosensors.
Jeffrey F. DiBerto, Katie Smart, Reid H.J. Olsen, Bryan L. Roth   +3 more
doaj   +1 more source

Understanding Bioluminescence in Dinoflagellates—How Far Have We Come? [PDF]

, 2013
Some dinoflagellates possess the remarkable genetic, biochemical, and cellular machinery to produce bioluminescence. Bioluminescent species appear to be ubiquitous in surface waters globally and include numerous cosmopolitan and harmful taxa ...
Debora Iglesias-Rodriguez, Iglesias-Rodriguez, M.D., Iglesias-Rodriguez, D., Valiadi, Martha, Valiadi, M., Martha Valiadi   +5 more
core   +1 more source

Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing

Nature Communications, 2016
The coupling of optogenetics with fluorescent Ca2+ sensors is confounded by sensitivity of optogenetic probes to light used to excite the sensors. Here the authors develop a Ca2+ sensor based on bioluminescence resonance energy transfer (BRET) that ...
Jie Yang, Derrick Cumberbatch, Samuel Centanni, Shu-qun Shi, Danny Winder, Donna Webb, Carl Hirschie Johnson   +6 more
doaj   +1 more source

Linear approaches to intramolecular Förster Resonance Energy Transfer probe measurements for quantitative modeling [PDF]

, 2011
Numerous unimolecular, genetically-encoded Forster Resonance Energy Transfer (FRET) probes for monitoring biochemical activities in live cells have been developed over the past decade.
Kurt I. Anderson, Kida, K., Katarzyna Kida, Birtwistle, M.R., Kriegsheim, Alexander von, Anderson, K.I., Kida Katarzyna, von Kriegsheim, A., Walter Kolch, Anderson Kurt I., Kida, Katarzyna, Schwarz, J.P., Marc R Birtwistle, Birtwistle Marc R., Birtwistle, Marc R., Alexander von Kriegsheim, Kurt I Anderson, von Kriegsheim Alexander, Juliane P Schwarz, Schwarz Juliane P., Kolch, W., Marc R. Birtwistle, Kolch Walter, Juliane P. Schwarz, et al.   +24 more
core   +1 more source

Equilibrium and Kinetic Measurements of Ligand Binding to HiBiT-tagged GPCRs on the Surface of Living Cells

Bio-Protocol, 2020
G-protein coupled receptors (GPCRs) remain at the forefront of drug discovery efforts. Detailed assessment of features contributing to GPCR ligand engagement in a physiologically relevant environment is imperative to the development of new therapeutics ...
Michelle Boursier, Sergiy Levin, Robin Hurst, Rachel Friedman Ohana   +3 more
doaj   +1 more source

Cerenkov radiation energy transfer (CRET) imaging: a novel method for optical imaging of PET isotopes in biological systems. [PDF]

PLoS ONE, 2010
Positron emission tomography (PET) allows sensitive, non-invasive analysis of the distribution of radiopharmaceutical tracers labeled with positron (β(+))-emitting radionuclides in small animals and humans.
Robin S Dothager, Reece J Goiffon, Erin Jackson, Scott Harpstrite, David Piwnica-Worms   +4 more
doaj   +1 more source

Full-Spectral Multiplexing of Bioluminescence Resonance Energy Transfer in Three TRPV Channels. [PDF]

Biophys J, 2017
Multiplexed bioluminescence resonance energy transfer (BRET) assays were developed to monitor the activation of several functional transient receptor potential (TRP) channels in live cells and in real time. We probed both TRPV1 intramolecular rearrangements and its interaction with Calmodulin (CaM) under activation by chemical agonists and temperature.
Ruigrok HJ, Shahid G, Goudeau B, Poulletier de Gannes F, Poque-Haro E, Hurtier A, Lagroye I, Vacher P, Arbault S, Sojic N, Veyret B, Percherancier Y.   +11 more
europepmc   +4 more sources