Results 61 to 70 of about 8,042,386 (212)

Bioluminescence-Based Energy Transfer Using Semiconductor Quantum Dots as Acceptors

Sensors, 2020
Bioluminescence resonance energy transfer (BRET) is the non-radiative transfer of energy from a bioluminescent protein donor to a fluorophore acceptor. It shares all the formalism of Förster resonance energy transfer (FRET) but differs in one key aspect:
Anirban Samanta, Igor L. Medintz
doaj   +1 more source

Quenching of Biotinylated Aequorin Bioluminescence by Dye-Labeled Avidin Conjugates:  Application to Homogeneous Bioluminescence Resonance Energy Transfer Assays

, 2016
Avidin conjugates containing the covalently attached dyes QSY-7 and dabcyl were prepared and shown to quench the bioluminescence of biotinylated aequorin.
Jeffrey A. Moore (2944254), Maciej Adamczyk (2944242), Kevin Shreder (2944251)   +2 more
core   +1 more source

Magneto‐NIR‐II‐Programmed Cascade Nanozymes Unlocking Blood–Brain Barrier Translocation and Autophagic Resistance in Glioblastoma

Advanced Science, EarlyView.
Magneto‐NIR‐II‐programmed NFSH nanozymes integrate magnetic blood–brain barrier (BBB) translocation, CD44 targeting, multimodal imaging, and cascade catalytic therapy for glioblastoma. Alternating magnetic fields and NIR‐II irradiation amplify ferroptosis, release H2S, suppress autophagic and mitophagic repair, and reshape the immune microenvironment ...
Ruocan Liu, Yundi Wu, Shuai Zhang, Hongjuan Zhao, Jiping Zheng, Shiyang Shao, Jianqiang Chen, Xilong Wu   +7 more
wiley   +1 more source

Bioluminescence resonance energy transfer to detect protein-protein interactions in live cells. [PDF]

Methods Mol Biol, 2015
Bioluminescence resonance energy transfer (BRET) is a valuable tool to detect protein-protein interactions. BRET utilizes bioluminescent and fluorescent protein tags with compatible emission and excitation properties, making it possible to examine resonance energy transfer when the tags are in close proximity (
Brown NE, Blumer JB, Hepler JR.
europepmc   +4 more sources

Determination of GLUT1 Oligomerization Parameters using Bioluminescent Förster Resonance Energy Transfer [PDF]

Scientific Reports, 2016
AbstractThe facilitated glucose transporter GLUT1 (SLC2A1) is an important mediator of glucose homeostasis in humans. Though it is found in most cell types to some extent, the level of GLUT1 expression across different cell types can vary dramatically.
Brendan Looyenga, Calvin VanOpstall, Zion Lee, Jed Bell, Evans Lodge, Katherine Wrobel, Eric Arnoys, Larry Louters   +7 more
openaire   +2 more sources

Repurposing a Small Molecule Plant Hormone as a Tunable ON‐Switch for CAR‐T Cell Immunotherapy

Advanced Science, EarlyView.
By engineering a receptor system integrating the plant auxin receptor AFB1 with its co‐receptor IAA7, we enable ligand‐dependent interactions triggered by the plant hormone auxins. This design allows rapid, reversible, and dose‐dependent T cell activation, resulting in potent cytotoxicity against B‐cell lymphoma in vitro and in vivo.
Hongxiang Zeng, Lei Zheng, Rongkun Tao, Sirui Zheng, Yanke Zhong, Yongxia Niu, Peng Zhang, Xiang Ma, Chen Yang, Min Zhang, Fuyu Duan, Jing Yang, Xuan Wei, Xiang Zhou, Zhenbiao Yang, Zhaoxi Sun, Qizhou Lian   +16 more
wiley   +1 more source

tracerDB: a crowdsourced fluorescent tracer database for target engagement analysis

Nature Communications
Investigating ligand-protein complexes is essential in the areas of chemical biology and drug discovery. However, detailed information on key reagents such as fluorescent tracers and associated data for the development of widely used bioluminescence ...
Johannes Dopfer, James D. Vasta, Susanne Müller, Stefan Knapp, Matthew B. Robers, Martin P. Schwalm   +5 more
doaj   +1 more source

Carrier‐Free Berberine/Nitidine Chloride Self‐Assembled Nanoparticles Induce Ferroptosis to Overcome Bortezomib Resistance in Multiple Myeloma

Advanced Science, EarlyView.
A schematic illustrating the fabrication process and ferroptosis‐mediated therapeutic mechanism of carrier‐free self‐assembled BBR/NC‐SAPs for overcoming bortezomib resistance in multiple myeloma. ABSTRACT Therapeutic relapse driven by bortezomib resistance represents a formidable clinical barrier in the management of multiple myeloma (MM).
Yiwen Lv, Shuang Liu, Hong Wu, Guiluan Li, Ying Rong, Guangchao Li, Yangmin Zhu, Qi Zhong, Ruiming Ou, Huijuan Shen, Zhi Liu, Jing Huang, Zhenwei Wang, Pei Lin, Qing Zhang, Guopan Yu, Zhao Yin   +16 more
wiley   +1 more source

Analysis of Protease Activity Using Quantum Dots and Resonance Energy Transfer

Theranostics, 2012
This review demonstrates the detection of protease activity based on the energy transfer of quantum dots (QDs). By incorporation of varying protease substrates into designed QD probes both in fluorescence resonance energy transfer (FRET) and ...
Gae Baik Kim, Young-Pil Kim
doaj  

Receptor-G Protein Interaction Studied by Bioluminescence Resonance Energy Transfer: Lessons From Protease-Activated Receptor 1

Frontiers in Endocrinology, 2012
Since its development, the bioluminescence resonance energy transfer (BRET) approach has been extensively applied to study G protein-coupled receptors (GPCRs) in real time and in live cells.
Mohammed Akli eAYOUB, Mohammed Akli eAYOUB, Mohammed Akli eAYOUB, Abdulrahman eAl-Senaidy, Jean-Philippe ePin, Jean-Philippe ePin   +5 more
doaj   +1 more source