Results 31 to 40 of about 7,863 (172)
Subcellular Imaging of Dynamic Protein Interactions by Bioluminescence Resonance Energy Transfer [PDF]
Biophysical Journal, 2008 Despite the fact that numerous studies suggest the existence of receptor multiprotein complexes, visualization and monitoring of the dynamics of such protein assemblies remain a challenge. In this study, we established appropriate conditions to consider spatiotemporally resolved images of such protein assemblies using bioluminescence resonance energy ...
Coulon, Vincent +6 more
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Resonance Energy Transfer Molecular Imaging Application in Biomedicine
Journal of Isotopes, 2016 Resonance energy transfer molecular imaging (RETI) can markedly improve signal intensity and tissue penetrating capacity of optical imaging, and have huge potential application in the deep-tissue optical imaging in vivo.
NIE Da-hong1,2;TANG Gang-hua1,3
doaj +1 more source
A beginner's guide to bioluminescence resonance energy transfer (BRET) [PDF]
The Biochemist, 2019 The Beginner's Guides are an ongoing series of articles in the magazine, each one covering a key technique and offering the scientifically literate but not necessarily expert audience a background briefing on the underlying science of a technique that is (or will be) widely used in molecular bioscience.
Etienne Sauvageau, Stephane Lefrancois
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cAMP Biosensors Based on Genetically Encoded Fluorescent/Luminescent Proteins
Biosensors, 2021 Cyclic adenosine monophosphate (cAMP) plays a key role in signal transduction pathways as a second messenger. Studies on the cAMP dynamics provided useful scientific insights for drug development and treatment of cAMP-related diseases such as some ...
Namdoo Kim, Seunghan Shin, Se Won Bae
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A Dual-Reporter Platform for Screening Tumor-Targeted Extracellular Vesicles
Pharmaceutics, 2022 Extracellular vesicle (EV)-mediated transfer of biomolecules plays an essential role in intercellular communication and may improve targeted drug delivery.
Masamitsu Kanada +3 more
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Luciferase-induced photoreductive uncaging of small-molecule effectors
Nature Communications, 2018 Bioluminescence resonance energy transfer (BRET) has been mostly employed in imaging applications. Here the authors use BRET to activate a ruthenium-based photocatalyst and perform a bioorthogonal chemical reaction, which can be used to uncage small ...
Eric Lindberg +3 more
doaj +1 more source
Biophysical detection of diversity and bias in GPCR function
Frontiers in Endocrinology, 2014 G protein-coupled receptors (GPCRs) function in complexes with a range of molecules and proteins including ligands, G proteins, arrestins, ubiquitin and other receptors.
Werner eJaeger +4 more
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Nature Communications, 2016 Cellular signaling processes often involve trafficking of receptors and other proteins between subcellular compartments. Here the authors demonstrate a method based on the concept of Enhanced bystander Bioluminescence Resonance Energy Transfer (EbBRET ...
Yoon Namkung +8 more
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Agonist and antagonist TRUPATH assays for G protein-coupled receptors
STAR Protocols, 2022 Summary: TRUPATH is a bioluminescence resonance energy transfer-based platform for quantifying G protein-coupled receptor activity via dissociation of heterotrimeric G protein biosensors.
Jeffrey F. DiBerto +3 more
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Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing
Nature Communications, 2016 The coupling of optogenetics with fluorescent Ca2+ sensors is confounded by sensitivity of optogenetic probes to light used to excite the sensors. Here the authors develop a Ca2+ sensor based on bioluminescence resonance energy transfer (BRET) that ...
Jie Yang +6 more
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