Multimeric Purinoceptor Detection by Bioluminescence Resonance Energy Transfer [PDF]
, 2019 Assays based on bioluminescence resonance energy transfer (BRET) provide a sensitive and simple method to study protein-protein interactions in live cells. Here we describe a protocol using BRET technique to investigate potential interactions between P2X subunits.
Vincent, Compan, François, Rassendren
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Bisdeoxycoelenterazine Derivatives for Improvement of Bioluminescence Resonance Energy Transfer Assays [PDF]
Journal of the American Chemical Society, 2007 We report here syntheses of bisdeoxycoelenterazine derivatives that show significantly improved kinetics of the bioluminescent reaction. All derivatives contain a protecting group at the carbonyl group of the imidazopyrazinone moiety that ensures slower light signal decay.
Jelena, Levi +3 more
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Subcellular Imaging of Dynamic Protein Interactions by Bioluminescence Resonance Energy Transfer [PDF]
Biophysical Journal, 2008 Despite the fact that numerous studies suggest the existence of receptor multiprotein complexes, visualization and monitoring of the dynamics of such protein assemblies remain a challenge. In this study, we established appropriate conditions to consider spatiotemporally resolved images of such protein assemblies using bioluminescence resonance energy ...
Coulon, Vincent +6 more
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Type-3 BRET, an improved competition-based bioluminescence resonance energy transfer assay. [PDF]
Biophys J, 2014 We show that in conventional, competition-based bioluminescence resonance energy transfer (BRET) assays of membrane protein stoichiometry, the presence of competitors can alter tagged-protein density and artifactually reduce energy transfer efficiency. A
Felce JH, Knox RG, Davis SJ.
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Bioluminescence Resonance Energy Transfer Reveals the Adrenocorticotropin (ACTH)-Induced Conformational Change of the Activated ACTH Receptor Complex in Living Cells [PDF]
, 2011 Bioluminescence resonance energy transfer analysis is used to study the interaction between melanocortin 2 receptor (MC2R) accessory protein and the MC2R and provides evidence for protein kinase A-dependent conformational changes in the receptor complex ...
Adrian J. L. Clark +9 more
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Fluorescence resonance energy transfer studies of protein interactions [PDF]
, 2008 This thesis presents an investigation of fluorescence resonance energy transfer (FRET) as a reporting signal for protein-protein interactions. Quantitative optical assays to measure protein binding, conjugation and deconjugation are developed and results
Martin, Sarah Friede
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A beginner's guide to bioluminescence resonance energy transfer (BRET) [PDF]
The Biochemist, 2019 The Beginner's Guides are an ongoing series of articles in the magazine, each one covering a key technique and offering the scientifically literate but not necessarily expert audience a background briefing on the underlying science of a technique that is (or will be) widely used in molecular bioscience.
Etienne Sauvageau, Stephane Lefrancois
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Bio-optimized energy transfer in densely packed fluorescent protein enables near-maximal luminescence and solid-state lasers [PDF]
, 2014 This work was supported by the US National Science Foundation (ECCS- 1101947), National Institutes of Health (P41EB015903, R21EB013761), Department of Defense (FA9550-11-1-0331), and the Korea National Research Foundation (R31-2008-000-10071-0).
Gather, Malte Christian, Yun, Seok Hyun
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cAMP Biosensors Based on Genetically Encoded Fluorescent/Luminescent Proteins
Biosensors, 2021 Cyclic adenosine monophosphate (cAMP) plays a key role in signal transduction pathways as a second messenger. Studies on the cAMP dynamics provided useful scientific insights for drug development and treatment of cAMP-related diseases such as some ...
Namdoo Kim, Seunghan Shin, Se Won Bae
doaj +1 more source
A Dual-Reporter Platform for Screening Tumor-Targeted Extracellular Vesicles
Pharmaceutics, 2022 Extracellular vesicle (EV)-mediated transfer of biomolecules plays an essential role in intercellular communication and may improve targeted drug delivery.
Masamitsu Kanada +3 more
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