In Vivo Analysis of Protein-Protein Interactions with Bioluminescence Resonance Energy Transfer (BRET): Progress and Prospects. [PDF]
Int J Mol Sci, 2016 Sun S, Yang X, Wang Y, Shen X.
europepmc +2 more sources
Developments in FRET- and BRET-Based Biosensors
Micromachines, 2022 Resonance energy transfer technologies have achieved great success in the field of analysis. Particularly, fluorescence resonance energy transfer (FRET) and bioluminescence resonance energy transfer (BRET) provide strategies to design tools for sensing ...
Yuexin Wu, Tianyu Jiang
doaj +1 more source
Flavoprotein miniSOG Cytotoxisity Can Be Induced By Bioluminescence Resonance Energy Transfer. [PDF]
Acta Naturae, 2016 In this study, we investigated the possibility of phototoxic flavoprotein miniSOG (photosensitizer) excitation in cancer cells by bioluminescence occurring when luciferase NanoLuc oxidizes its substrate, furimazine. We have shown that the phototoxic flavoprotein miniSOG expressed in eukaryotic cells in fusion with NanoLuc luciferase is activated in the
Shramova EI +4 more
europepmc +4 more sources
Multiplexing of multicolor bioluminescence resonance energy transfer. [PDF]
Biophys J, 2010 Bioluminescence resonance energy transfer (BRET) is increasingly being used to monitor protein-protein interactions and cellular events in cells. However, the ability to monitor multiple events simultaneously is limited by the spectral properties of the existing BRET partners.
Breton B +5 more
europepmc +4 more sources
Biosensing and imaging based on bioluminescence resonance energy transfer [PDF]
Current Opinion in Biotechnology, 2009 Bioluminescence resonance energy transfer (BRET) operates with biochemical energy generated by bioluminescent proteins to excite fluorophores and offers additional advantages over fluorescence energy transfer (FRET) for in vivo imaging and biosensing.
Zuyong, Xia, Jianghong, Rao
openaire +2 more sources
Multiple GPCR Functional Assays Based on Resonance Energy Transfer Sensors
Frontiers in Cell and Developmental Biology, 2021 G protein-coupled receptors (GPCRs) represent one of the largest membrane protein families that participate in various physiological and pathological activities.
Yiwei Zhou +5 more
doaj +1 more source
Multimeric Purinoceptor Detection by Bioluminescence Resonance Energy Transfer [PDF]
, 2019 Assays based on bioluminescence resonance energy transfer (BRET) provide a sensitive and simple method to study protein-protein interactions in live cells. Here we describe a protocol using BRET technique to investigate potential interactions between P2X subunits.
Vincent, Compan, François, Rassendren
openaire +2 more sources
Resistance to kinase inhibition through shortened target engagement
Molecular & Cellular Oncology, 2022 Imatinib, a selective inhibitor of the breakpoint cluster region (BCR)-ABL kinase, is the poster child for targeted cancer therapeutics. However, its efficacy is limited by resistance mutations.
Aziz M. Rangwala +4 more
doaj +1 more source
Bisdeoxycoelenterazine Derivatives for Improvement of Bioluminescence Resonance Energy Transfer Assays [PDF]
Journal of the American Chemical Society, 2007 We report here syntheses of bisdeoxycoelenterazine derivatives that show significantly improved kinetics of the bioluminescent reaction. All derivatives contain a protecting group at the carbonyl group of the imidazopyrazinone moiety that ensures slower light signal decay.
Jelena, Levi +3 more
openaire +2 more sources
Biophysica, 2022 Bioluminescence resonance energy transfer (BRET) seems to be a promising biophysical technique to study protein–protein interactions within living cells due to a very specific reaction of bioluminescence that essentially decreases the background of other
Victor Marchenkov +4 more
doaj +1 more source