Results 21 to 30 of about 7,702 (129)
Multiple GPCR Functional Assays Based on Resonance Energy Transfer Sensors
Frontiers in Cell and Developmental Biology, 2021 G protein-coupled receptors (GPCRs) represent one of the largest membrane protein families that participate in various physiological and pathological activities.
Yiwei Zhou +5 more
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Resistance to kinase inhibition through shortened target engagement
Molecular & Cellular Oncology, 2022 Imatinib, a selective inhibitor of the breakpoint cluster region (BCR)-ABL kinase, is the poster child for targeted cancer therapeutics. However, its efficacy is limited by resistance mutations.
Aziz M. Rangwala +4 more
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Biophysica, 2022 Bioluminescence resonance energy transfer (BRET) seems to be a promising biophysical technique to study protein–protein interactions within living cells due to a very specific reaction of bioluminescence that essentially decreases the background of other
Victor Marchenkov +4 more
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Resonance Energy Transfer Molecular Imaging Application in Biomedicine
Journal of Isotopes, 2016 Resonance energy transfer molecular imaging (RETI) can markedly improve signal intensity and tissue penetrating capacity of optical imaging, and have huge potential application in the deep-tissue optical imaging in vivo.
NIE Da-hong1,2;TANG Gang-hua1,3
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cAMP Biosensors Based on Genetically Encoded Fluorescent/Luminescent Proteins
Biosensors, 2021 Cyclic adenosine monophosphate (cAMP) plays a key role in signal transduction pathways as a second messenger. Studies on the cAMP dynamics provided useful scientific insights for drug development and treatment of cAMP-related diseases such as some ...
Namdoo Kim, Seunghan Shin, Se Won Bae
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A Dual-Reporter Platform for Screening Tumor-Targeted Extracellular Vesicles
Pharmaceutics, 2022 Extracellular vesicle (EV)-mediated transfer of biomolecules plays an essential role in intercellular communication and may improve targeted drug delivery.
Masamitsu Kanada +3 more
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Luciferase-induced photoreductive uncaging of small-molecule effectors
Nature Communications, 2018 Bioluminescence resonance energy transfer (BRET) has been mostly employed in imaging applications. Here the authors use BRET to activate a ruthenium-based photocatalyst and perform a bioorthogonal chemical reaction, which can be used to uncage small ...
Eric Lindberg +3 more
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Biophysical detection of diversity and bias in GPCR function
Frontiers in Endocrinology, 2014 G protein-coupled receptors (GPCRs) function in complexes with a range of molecules and proteins including ligands, G proteins, arrestins, ubiquitin and other receptors.
Werner eJaeger +4 more
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Nature Communications, 2016 Cellular signaling processes often involve trafficking of receptors and other proteins between subcellular compartments. Here the authors demonstrate a method based on the concept of Enhanced bystander Bioluminescence Resonance Energy Transfer (EbBRET ...
Yoon Namkung +8 more
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Agonist and antagonist TRUPATH assays for G protein-coupled receptors
STAR Protocols, 2022 Summary: TRUPATH is a bioluminescence resonance energy transfer-based platform for quantifying G protein-coupled receptor activity via dissociation of heterotrimeric G protein biosensors.
Jeffrey F. DiBerto +3 more
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