Novel tools for use in Bioluminescence Resonance Energy Transfer (BRET) assays
, 2009 Recent advances in imaging assays based on bioluminescence resonance energy transfer (BRET) have made it possible to study protein/protein interactions in living cells under physiological conditions.
Alessandra Baragli +7 more
core +6 more sources
Self-illumination of Carbon Dots by Bioluminescence Resonance Energy Transfer. [PDF]
Sci Rep, 2019 AbstractCarbon-dots (CDs), the emerging fluorescent nanoparticles, show special multicolor properties, chemical stability, and biocompatibility, and are considered as the new and advanced imaging probe in replacement of molecular fluorophores and semiconductor quantum dots.
Song J, Zhang J.
europepmc +4 more sources
Multiresolution Imaging Using Bioluminescence Resonance Energy Transfer Identifies Distinct Biodistribution Profiles of Extracellular Vesicles and Exomeres with Redirected Tropism. [PDF]
Adv Sci (Weinh), 2020 Wu AY +18 more
europepmc +2 more sources
Ratiometric Zinc Biosensor Based on Bioluminescence Resonance Energy Transfer: Trace Metal Ion Determination with Tunable Response. [PDF]
Int J Mol Sci, 2022 Determination of metal ions such as zinc in solution remains an important task in analytical and biological chemistry. We describe a novel zinc ion biosensing approach using a carbonic anhydrase–Oplophorus luciferase fusion protein that employs ...
Matveeva EG +6 more
europepmc +2 more sources
Developments in FRET- and BRET-Based Biosensors
Micromachines, 2022 Resonance energy transfer technologies have achieved great success in the field of analysis. Particularly, fluorescence resonance energy transfer (FRET) and bioluminescence resonance energy transfer (BRET) provide strategies to design tools for sensing ...
Yuexin Wu, Tianyu Jiang
doaj +1 more source
Flavoprotein miniSOG Cytotoxisity Can Be Induced By Bioluminescence Resonance Energy Transfer. [PDF]
Acta Naturae, 2016 In this study, we investigated the possibility of phototoxic flavoprotein miniSOG (photosensitizer) excitation in cancer cells by bioluminescence occurring when luciferase NanoLuc oxidizes its substrate, furimazine. We have shown that the phototoxic flavoprotein miniSOG expressed in eukaryotic cells in fusion with NanoLuc luciferase is activated in the
Shramova EI +4 more
europepmc +4 more sources
In Vivo Analysis of Protein-Protein Interactions with Bioluminescence Resonance Energy Transfer (BRET): Progress and Prospects. [PDF]
Int J Mol Sci, 2016 Sun S, Yang X, Wang Y, Shen X.
europepmc +2 more sources
Biosensing and imaging based on bioluminescence resonance energy transfer [PDF]
Current Opinion in Biotechnology, 2009 Bioluminescence resonance energy transfer (BRET) operates with biochemical energy generated by bioluminescent proteins to excite fluorophores and offers additional advantages over fluorescence energy transfer (FRET) for in vivo imaging and biosensing.
Zuyong, Xia, Jianghong, Rao
openaire +2 more sources
Multiple GPCR Functional Assays Based on Resonance Energy Transfer Sensors
Frontiers in Cell and Developmental Biology, 2021 G protein-coupled receptors (GPCRs) represent one of the largest membrane protein families that participate in various physiological and pathological activities.
Yiwei Zhou +5 more
doaj +1 more source
Resistance to kinase inhibition through shortened target engagement
Molecular & Cellular Oncology, 2022 Imatinib, a selective inhibitor of the breakpoint cluster region (BCR)-ABL kinase, is the poster child for targeted cancer therapeutics. However, its efficacy is limited by resistance mutations.
Aziz M. Rangwala +4 more
doaj +1 more source