Results 71 to 80 of about 131,173 (232)

The potential for biased signalling in the P2Y receptor family of GPCRs

British Journal of Pharmacology, EarlyView.
The purinergic receptor family is primarily activated by nucleotides, and contains members of both the G protein coupled‐receptor (GPCR) superfamily (P1 and P2Y) and ligand‐gated ion channels (P2X). The P2Y receptors are widely expressed in the human body, and given the ubiquitous nature of nucleotides, purinergic signalling is involved with a plethora
Claudia M. Sisk, Simon Pitchford, Graham Ladds   +2 more
wiley   +1 more source

AIMTOR, a BRET biosensor for live imaging, reveals subcellular mTOR signaling and dysfunctions

BMC Biology, 2020
mTOR signaling is an essential nutrient and energetic sensing pathway. Here we describe AIMTOR, a sensitive genetically encoded BRET (Bioluminescent Resonance Energy Transfer) biosensor to study mTOR activity in living cells.
Nathalie Bouquier, Enora Moutin, L. Tintignac, Amandine Reverbel, E. Jublanc, M. Sinnreich, Yan Chastagnier, J. Averous, P. Fafournoux, C. Verpelli, T. Boeckers, G. Carnac, Julie Perroy, V. Ollendorff   +13 more
semanticscholar   +1 more source

Image Processing for Bioluminescence Resonance Energy Transfer Measurement—BRET-Analyzer

Frontiers in Computational Neuroscience, 2018
A growing number of tools now allow live recordings of various signaling pathways and protein-protein interaction dynamics in time and space by ratiometric measurements, such as Bioluminescence Resonance Energy Transfer (BRET) Imaging.
Yan Chastagnier, Yan Chastagnier, Yan Chastagnier, Enora Moutin, Enora Moutin, Enora Moutin, Anne-Laure Hemonnot, Anne-Laure Hemonnot, Anne-Laure Hemonnot, Julie Perroy, Julie Perroy, Julie Perroy   +11 more
doaj   +1 more source

NanoBRET in C. elegans illuminates functional receptor interactions in real time

BMC Molecular and Cell Biology, 2022
Background Protein-protein interactions form the basis of every organism and thus, investigating their dynamics, intracellular protein localization, trafficking and interactions of distinct proteins such as receptors and their ligand-binding are of ...
Victoria Elisabeth Groß, Miron Mikhailowitsch Gershkovich, Torsten Schöneberg, Anette Kaiser, Simone Prömel   +4 more
doaj   +1 more source

Systematic profiling reveals broad G protein coupling and context‐dependent modulation of cyclic AMP by the orphan receptor GPR139

British Journal of Pharmacology, EarlyView.
Background and Purpose G protein‐coupled receptors (GPCRs) are major drug targets, yet many orphan receptors remain poorly characterized. GPR139 has been implicated in CNS disorders, including schizophrenia and depression, but its signalling mechanisms remain unclear.
Boris Trapkov, Hans Bräuner, Alexander Sebastian Hauser   +2 more
wiley   +1 more source

Paper‐Based Antibody Detection Devices Using Bioluminescent BRET‐Switching Sensor Proteins

Angewandte Chemie, 2018
This work reports on fully integrated “sample‐in‐signal‐out” microfluidic paper‐based analytical devices (μPADs) relying on bioluminescence resonance energy transfer (BRET) switches for analyte recognition and colorimetric signal generation.
Keisuke Tenda, Benice van Gerven, R. Arts, Y. Hiruta, M. Merkx, D. Citterio   +5 more
semanticscholar   +1 more source

Luciferase-induced photoreductive uncaging of small-molecule effectors

Nature Communications, 2018
Bioluminescence resonance energy transfer (BRET) has been mostly employed in imaging applications. Here the authors use BRET to activate a ruthenium-based photocatalyst and perform a bioorthogonal chemical reaction, which can be used to uncage small ...
Eric Lindberg, Simona Angerani, Marcello Anzola, Nicolas Winssinger   +3 more
doaj   +1 more source

Spanish Translation, Cultural Adaptation and Validation of the Person‐Centred Practice Inventory‐Care (PCPI‐C): Enhancing Collaborative Care and Patient Involvement

Journal of Advanced Nursing, EarlyView.
ABSTRACT Aim(s) To translate, culturally adapt and validate the first Spanish version of the Person‐centred Practice Inventory‐Care (PCPI‐C) instrument. Design Cross‐cultural adaptation and psychometric validation. Methods Two‐phase research design: (1) the PCPI‐C's translation and cultural adaptation from English to Spanish following the ‘Translation ...
Ana Choperena, Ana Carvajal‐Valcárcel, Mónica Vázquez‐Calatayud, Juan Gamero‐Salinas, Begoña Errasti‐Ibarrondo, Virginia La Rosa‐Salas, Brendan McCormack, Vaibhav Tyagi, Edgar Benitez, Marta Lizarbe‐Chocarro   +9 more
wiley   +1 more source

Functional selectivity profiling of the angiotensin II type 1 receptor using pathway-wide BRET signaling sensors

Science Signaling, 2018
Bioluminescence-based biosensors enable comprehensive profiling of G protein–coupled receptor signaling bias. Biosensors for GPCR-biased signaling Upon binding to ligands, G protein–coupled receptors (GPCRs) stimulate heterotrimeric G proteins and ...
Y. Namkung, Christian LeGouill, Sahil Kumar, Yubo Cao, Larissa B. Teixeira, V. Lukasheva, Jenna Giubilaro, S. C. Simões, Jean-Michel Longpré, D. Devost, T. Hébert, G. Piñeyro, R. Leduc, C. Costa-Neto, M. Bouvier, S. Laporte   +15 more
semanticscholar   +1 more source

Application of quantum dot nanoparticles for potential non-invasive bio-imaging of mammalian spermatozoa

Journal of Nanobiotechnology, 2012
Background Various obstacles are encountered by mammalian spermatozoa during their journey through the female genital tract, and only few or none will reach the site of fertilization.
Feugang Jean M, Youngblood Ramey C, Greene Jonathan M, Fahad Abed S, Monroe William A, Willard Scott T, Ryan Peter L   +6 more
doaj   +1 more source