Experimental determination of the bioluminescence resonance energy transfer (BRET) Förster distances of NanoBRET and red-shifted BRET pairs. [PDF]
Anal Chim Acta X, 2020 Bioluminescence Resonance Energy Transfer (BRET) is widely applied to study protein-protein interactions, as well as increasingly to monitor both ligand binding and molecular rearrangements.
Weihs F, Wang J, Pfleger KDG, Dacres H.
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In Vivo Analysis of Protein-Protein Interactions with Bioluminescence Resonance Energy Transfer (BRET): Progress and Prospects. [PDF]
Int J Mol Sci, 2016 Proteins are the elementary machinery of life, and their functions are carried out mostly by molecular interactions. Among those interactions, protein–protein interactions (PPIs) are the most important as they participate in or mediate all essential ...
Sun S, Yang X, Wang Y, Shen X.
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Bioluminescence Resonance Energy Transfer (BRET) Assay for Determination of Molecular Interactions in Living Cells [PDF]
Bio-Protocol, 2017 The bioluminescence resonance energy transfer (BRET) assay can be used as an indicator of molecular approximation and/or interaction. A significant resonance energy transfer signal is generated when the acceptor, having the appropriate spectral overlap ...
Kaleeckal Harikumar +5 more
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Bio-Protocol, 2019 Detecting protein-protein interactions by co-immunoprecipitation provided a major advancement in the immunology research field. In the G-protein-coupled receptors (GPCRs) research field, colocalization and co-immunoprecipitation were used to detect ...
Irene Reyes-Resina +3 more
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Real‐Time Visualization of Isoform‐Specific RAF‐KRAS Interactions in Living Cells Using FRET‐BRET Hybrid Biosensors [PDF]
Advanced ScienceThe RAS–RAF–MEK–ERK signaling cascade is a central component of the mitogen‐activated protein kinase (MAPK) pathway, regulating cell proliferation, differentiation, and survival, and is frequently dysregulated in cancer.
Jeong‐Min Go +12 more
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Bioluminescence Resonance Energy Transfer (BRET)-Mediated Protein Release from Self-Illuminating Photoresponsive Biomaterials. [PDF]
J Am Chem SocPhototriggered release of various cargos, including soluble protein factors and small molecules, has the potential to correct aberrant biological events by offering spatiotemporal control over local therapeutic levels. However, the poor penetration depth
Rapp TL +3 more
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Predicting response to platinum and non-platinum drugs through bioluminescence resonance energy transfer (BRET) based bio-molecular interactions in platinum resistant epithelial ovarian cancer. [PDF]
Transl Oncol, 2021 Highlights • Dynamic monitoring of therapy induced interactions between target/partners of ERK and AKT kinases in drug resistant ovarian cancer cell utilizing two improved nanoBRET sensors.• Delineation of the dynamics of ERK1/2 and AKT activation in ...
Bishnu A +5 more
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In-cell bioluminescence resonance energy transfer (BRET)-based assay uncovers ceritinib and CA-074 as SARS-CoV-2 papain-like protease inhibitors. [PDF]
J Enzyme Inhib Med ChemPapain-like protease (PLpro) is an attractive anti-coronavirus target. The development of PLpro inhibitors, however, is hampered by the limitations of the existing PLpro assay and the scarcity of validated active compounds.
Li M +8 more
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Biophysica, 2022 Bioluminescence resonance energy transfer (BRET) seems to be a promising biophysical technique to study protein–protein interactions within living cells due to a very specific reaction of bioluminescence that essentially decreases the background of other
Victor Marchenkov +4 more
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SLAS Discovery, 2008 The bioluminescence resonance energy transfer (BRET) technique has become extremely popular for studying protein-protein interactions in living cells and real time.
M. Kočan +4 more
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