Bioluminescence Resonance Energy Transfer (BRET) Assay for Determination of Molecular Interactions in Living Cells [PDF]
Bio-Protocol, 2017 The bioluminescence resonance energy transfer (BRET) assay can be used as an indicator of molecular approximation and/or interaction. A significant resonance energy transfer signal is generated when the acceptor, having the appropriate spectral overlap ...
Kaleeckal Harikumar +5 more
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In Vivo Analysis of Protein-Protein Interactions with Bioluminescence Resonance Energy Transfer (BRET): Progress and Prospects. [PDF]
Int J Mol Sci, 2016 Proteins are the elementary machinery of life, and their functions are carried out mostly by molecular interactions. Among those interactions, protein–protein interactions (PPIs) are the most important as they participate in or mediate all essential ...
Sun S, Yang X, Wang Y, Shen X.
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Analysis of RXR/THR and RXR/PPARG2 heterodimerization by bioluminescence resonance energy transfer (BRET). [PDF]
PLoS ONE, 2013 BACKGROUND: Nuclear receptors (NR) regulate transcription of genes involved in many biological processes such as development, cell proliferation, differentiation and cell death.
Miquel Mulero +4 more
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Identification of Heteroreceptors Complexes and Signal Transduction Events Using Bioluminescence Resonance Energy Transfer (BRET) [PDF]
Bio-Protocol, 2019 Detecting protein-protein interactions by co-immunoprecipitation provided a major advancement in the immunology research field. In the G-protein-coupled receptors (GPCRs) research field, colocalization and co-immunoprecipitation were used to detect ...
Irene Reyes-Resina +3 more
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Real‐Time Visualization of Isoform‐Specific RAF‐KRAS Interactions in Living Cells Using FRET‐BRET Hybrid Biosensors [PDF]
Advanced ScienceThe RAS–RAF–MEK–ERK signaling cascade is a central component of the mitogen‐activated protein kinase (MAPK) pathway, regulating cell proliferation, differentiation, and survival, and is frequently dysregulated in cancer.
Jeong‐Min Go +12 more
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Enhanced Molecular Tension Sensor Based on Bioluminescence Resonance Energy Transfer (BRET). [PDF]
ACS Sens, 2020 Molecular tension sensors measure piconewton forces experienced by individual proteins in the context of the cellular microenvironment. Current genetically encoded tension sensors use FRET to report on extension of a deformable peptide encoded in a cellular protein of interest.
Aird EJ +3 more
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Genetically Encoded RNA-Based Bioluminescence Resonance Energy Transfer (BRET) Sensors. [PDF]
ACS Sens, 2023 Abstract RNA-based nanostructures and molecular devices have become popular for developing biosensors and genetic regulators. These programmable RNA nanodevices can be genetically encoded and modularly engineered to detect various cellular targets and then induce output signals, most often a fluorescence readout ...
Mi L +7 more
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Experimental determination of the bioluminescence resonance energy transfer (BRET) Förster distances of NanoBRET and red-shifted BRET pairs. [PDF]
Anal Chim Acta X, 2020 Bioluminescence Resonance Energy Transfer (BRET) is widely applied to study protein-protein interactions, as well as increasingly to monitor both ligand binding and molecular rearrangements. The Förster distance (R0) describes the physical distance between the two chromophores at which 50% of the maximal energy transfer occurs and it depends on the ...
Weihs F, Wang J, Pfleger KDG, Dacres H.
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Developments in FRET- and BRET-Based Biosensors
Micromachines, 2022 Resonance energy transfer technologies have achieved great success in the field of analysis. Particularly, fluorescence resonance energy transfer (FRET) and bioluminescence resonance energy transfer (BRET) provide strategies to design tools for sensing ...
Yuexin Wu, Tianyu Jiang
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AB-MDMSBA-Not a Synthetic Cannabinoid Receptor Agonist. [PDF]
Pharmacol Res PerspectThis study uses classical in vitro pharmacology approaches to establish that AB‐MDMSBA does not bind to or activate the cannabinoid CB1 receptor and therefore should not be classified as a synthetic cannabinoid receptor agonist. ABSTRACT Synthetic cannabinoids remain one of the most prominent classes of New Psychoactive Substances (NPS).
Patel M +4 more
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