Results 21 to 30 of about 5,054 (183)

Application of bioluminescence resonance energy transfer-based cell tracking approach in bone tissue engineering

Journal of Tissue Engineering, 2021
Bioluminescent imaging (BLI) has emerged as a popular in vivo tracking modality in bone regeneration studies stemming from its clear advantages: non-invasive, real-time, and inexpensive. We recently adopted bioluminescence resonance energy transfer (BRET)
Lufei Wang, Dong Joon Lee, Han Han, Lixing Zhao, Hiroshi Tsukamoto, Yong-IL Kim, Adele M Musicant, Kshitij Parag-Sharma, Xiangxiang Hu, Henry C Tseng, Jen-Tsan Chi, Zhengyan Wang, Antonio L Amelio, Ching-Chang Ko   +13 more
doaj   +2 more sources

Comparison of static and microfluidic protease assays using modified bioluminescence resonance energy transfer chemistry.

PLoS ONE, 2014
BackgroundFluorescence and bioluminescence resonance energy transfer (F/BRET) are two forms of Förster resonance energy transfer, which can be used for optical transduction of biosensors.
Nan Wu, Helen Dacres, Alisha Anderson, Stephen C Trowell, Yonggang Zhu   +4 more
doaj   +3 more sources

Bioluminescence-Based Energy Transfer Using Semiconductor Quantum Dots as Acceptors

Sensors, 2020
Bioluminescence resonance energy transfer (BRET) is the non-radiative transfer of energy from a bioluminescent protein donor to a fluorophore acceptor. It shares all the formalism of Förster resonance energy transfer (FRET) but differs in one key aspect:
Anirban Samanta, Igor L. Medintz
doaj   +2 more sources

Bioluminescence Resonance Energy Transfer as a Method to Study Protein-Protein Interactions: Application to G Protein Coupled Receptor Biology

Molecules, 2019
The bioluminescence resonance energy transfer (BRET) approach involves resonance energy transfer between a light-emitting enzyme and fluorescent acceptors.
Chayma El Khamlichi, Flora Reverchon-Assadi, Nadège Hervouet-Coste, Lauren Blot, Eric Reiter, Séverine Morisset-Lopez   +5 more
doaj   +2 more sources

Bioluminescence Resonance Energy Transfer (BRET) Assay to Measure Gq Recruitment to the Ghrelin Receptor

This protocol chapter outlines a guide for investigating ghrelin receptor activation of Gq signaling using Bioluminescence Resonance Energy Transfer (BRET).
Jamaluddin, Aqfan; id_orcid
core   +3 more sources

Bioluminescence resonance energy transfer (BRET) to detect the interactions between kappa opioid receptor and non visual arrestins.

, 2014
Bioluminescence resonance energy transfer (BRET) is a very sensitive technique employed to study protein-protein interactions, including G-protein-coupled receptors (GPCRs) hetero- and homo-dimerization.
BEDINI, ANDREA, Andrea Bedini
core   +6 more sources

Application of bioluminescence resonance energy transfer assays in primary mouse neuronal cultures

STAR Protocols
Summary: Bioluminescence resonance energy transfer (BRET) is widely employed for real-time monitoring of G protein-coupled receptor activity, interactions, and trafficking in heterologous cell lines, yet its use in neuronal systems remains limited. Here,
Hanh T.M. Hoang, Kiran George, Mohiuddin Ahmad   +2 more
doaj   +2 more sources

Analysis of Protease Activity Using Quantum Dots and Resonance Energy Transfer

Theranostics, 2012
This review demonstrates the detection of protease activity based on the energy transfer of quantum dots (QDs). By incorporation of varying protease substrates into designed QD probes both in fluorescence resonance energy transfer (FRET) and ...
Gae Baik Kim, Young-Pil Kim
doaj   +2 more sources

Bioluminescence resonance energy transfer (BRET) imaging in plant seedlings and mammalian cells. [PDF]

Methods Mol Biol, 2011
Bioluminescence resonance energy transfer (BRET) has become a widely used technique to monitor protein-protein interactions. It involves resonance energy transfer between a bioluminescent donor and a fluorescent acceptor. Because the donor emits photons intrinsically, fluorescence excitation is unnecessary.
Xie Q, Soutto M, Xu X, Zhang Y, Johnson CH.   +4 more
europepmc   +4 more sources

A beginner's guide to bioluminescence resonance energy transfer (BRET) [PDF]

The Biochemist, 2019
The Beginner's Guides are an ongoing series of articles in the magazine, each one covering a key technique and offering the scientifically literate but not necessarily expert audience a background briefing on the underlying science of a technique that is (or will be) widely used in molecular bioscience.
Etienne Sauvageau, Stephane Lefrancois
openaire   +1 more source