Real‐Time Visualization of Isoform‐Specific RAF‐KRAS Interactions in Living Cells Using FRET‐BRET Hybrid Biosensors [PDF]
Advanced ScienceThe RAS–RAF–MEK–ERK signaling cascade is a central component of the mitogen‐activated protein kinase (MAPK) pathway, regulating cell proliferation, differentiation, and survival, and is frequently dysregulated in cancer.
Jeong‐Min Go +12 more
doaj +2 more sources
BRET-Based Approaches for Ion Channels: Emerging Applications and Future Directions. [PDF]
Arch Pharm (Weinheim)Bioluminescence resonance energy transfer (BRET) is a versatile method for studying ligand–protein interactions. Its compatibility with high‐throughput screening and real‐time kinetics has made it widely used in drug discovery. Here, we summarize recent applications of BRET‐based methods for studying ligand binding to ion channels, highlighting key ...
Kock N +6 more
europepmc +2 more sources
Synthetic fluorescent proteins: Bridging advanced imaging technologies and clinical practice. [PDF]
Clin Transl MedClinical and Translational Medicine, Volume 16, Issue 9, September 2026.
Chen Z, Chen X, Yang Y.
europepmc +2 more sources
A beginner's guide to bioluminescence resonance energy transfer (BRET) [PDF]
The Biochemist, 2019 The Beginner's Guides are an ongoing series of articles in the magazine, each one covering a key technique and offering the scientifically literate but not necessarily expert audience a background briefing on the underlying science of a technique that is (or will be) widely used in molecular bioscience.
Etienne Sauvageau, Stephane Lefrancois
openaire +1 more source
Improved HaloTag Ligand Enables BRET Imaging With NanoLuc
Frontiers in Chemistry, 2020 Bioluminescence resonance energy transfer (BRET) from an exceptionally bright luciferase, NanoLuc, to a fluorescent HaloTag ligand is gaining momentum to monitor molecular interactions.
Ovia Margaret Thirukkumaran +8 more
doaj +1 more source
Bioluminescence Resonance Energy Transfer Reveals the Adrenocorticotropin (ACTH)-Induced Conformational Change of the Activated ACTH Receptor Complex in Living Cells [PDF]
, 2011 Bioluminescence resonance energy transfer analysis is used to study the interaction between melanocortin 2 receptor (MC2R) accessory protein and the MC2R and provides evidence for protein kinase A-dependent conformational changes in the receptor complex ...
Adrian J. L. Clark +9 more
core +1 more source
Luciferase–Rose Bengal conjugates for singlet oxygen generation by bioluminescence resonance energy transfer [PDF]
, 2017 Luciferase–Rose Bengal conjugates generated reactive oxygen species (ROS) inside cells via bioluminescence resonance energy transfer (BRET) without external light irradiation.
Sei Kwang Hahn +5 more
core +2 more sources
Luciferase-induced photoreductive uncaging of small-molecule effectors
Nature Communications, 2018 Bioluminescence resonance energy transfer (BRET) has been mostly employed in imaging applications. Here the authors use BRET to activate a ruthenium-based photocatalyst and perform a bioorthogonal chemical reaction, which can be used to uncage small ...
Eric Lindberg +3 more
doaj +1 more source
Molecules, 2019 The bioluminescence resonance energy transfer (BRET) approach involves resonance energy transfer between a light-emitting enzyme and fluorescent acceptors.
Chayma El Khamlichi +5 more
doaj +1 more source
Scientific Reports, 2022 Bioluminescence resonance energy transfer (BRET) saturation is a method of studying protein–protein interaction (PPI) upon quantification of the dependence of the BRET signal on the acceptor/donor (A:D) expression ratio.
Benoit Besson +5 more
doaj +1 more source