Results 21 to 30 of about 6,388 (174)
AB-MDMSBA-Not a Synthetic Cannabinoid Receptor Agonist. [PDF]
Pharmacol Res PerspectThis study uses classical in vitro pharmacology approaches to establish that AB‐MDMSBA does not bind to or activate the cannabinoid CB1 receptor and therefore should not be classified as a synthetic cannabinoid receptor agonist. ABSTRACT Synthetic cannabinoids remain one of the most prominent classes of New Psychoactive Substances (NPS).
Patel M +4 more
europepmc +2 more sources
Bioluminescence resonance energy transfer (BRET) imaging of protein-protein interactions within deep tissues of living subjects. [PDF]
Proc Natl Acad Sci U S A, 2011 Dragulescu-Andrasi A +4 more
europepmc +2 more sources
A bioluminescence resonance energy transfer (BRET) system: application to interacting circadian clock proteins. [PDF]
Proceedings of the National Academy of Sciences, 1999 We describe a method for assaying protein interactions that offers some attractive advantages over previous assays. This method, called bioluminescence resonance energy transfer (BRET), uses a bioluminescent luciferase that is genetically fused to one candidate protein, and a green fluorescent protein mutant fused to another protein of interest ...
Yao Xu, D. Piston, C. Johnson
semanticscholar +3 more sources
Developments in FRET- and BRET-Based Biosensors
Micromachines, 2022 Resonance energy transfer technologies have achieved great success in the field of analysis. Particularly, fluorescence resonance energy transfer (FRET) and bioluminescence resonance energy transfer (BRET) provide strategies to design tools for sensing ...
Yuexin Wu, Tianyu Jiang
doaj +1 more source
Imaging protein interactions with bioluminescence resonance energy transfer (BRET) in plant and mammalian cells and tissues [PDF]
Proceedings of the National Academy of Sciences, 2007 FRET is a well established method for cellular and subcellular imaging of protein interactions. However, FRET obligatorily necessitates fluorescence excitation with its concomitant problems of photobleaching, autofluorescence, phototoxicity, and undesirable stimulation of photobiological processes.
Xiaodong Xu +6 more
semanticscholar +3 more sources
A beginner's guide to bioluminescence resonance energy transfer (BRET) [PDF]
The Biochemist, 2019 The Beginner's Guides are an ongoing series of articles in the magazine, each one covering a key technique and offering the scientifically literate but not necessarily expert audience a background briefing on the underlying science of a technique that is (or will be) widely used in molecular bioscience.
Etienne Sauvageau, Stephane Lefrancois
openaire +1 more source
Genetically Encoded RNA-based Bioluminescence Resonance Energy Transfer (BRET) Sensors
bioRxiv, 2022 RNA-based nanostructures and molecular devices have become popular for developing biosensors and genetic regulators. These programmable RNA nanodevices can be genetically encoded and modularly engineered to detect various cellular targets and then induce
Lan Mi +7 more
semanticscholar +1 more source
Improved HaloTag Ligand Enables BRET Imaging With NanoLuc
Frontiers in Chemistry, 2020 Bioluminescence resonance energy transfer (BRET) from an exceptionally bright luciferase, NanoLuc, to a fluorescent HaloTag ligand is gaining momentum to monitor molecular interactions.
Ovia Margaret Thirukkumaran +8 more
doaj +1 more source
Biosensors, 2019 Bioluminescence resonance energy transfer (BRET) techniques offer a high degree of sensitivity, reliability and ease of use for their application to sensing biomolecules.
Eugene Hwang, Jisu Song, Jin Zhang
doaj +1 more source
Luciferase-induced photoreductive uncaging of small-molecule effectors
Nature Communications, 2018 Bioluminescence resonance energy transfer (BRET) has been mostly employed in imaging applications. Here the authors use BRET to activate a ruthenium-based photocatalyst and perform a bioorthogonal chemical reaction, which can be used to uncage small ...
Eric Lindberg +3 more
doaj +1 more source