Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing
Nature Communications, 2016 The coupling of optogenetics with fluorescent Ca2+ sensors is confounded by sensitivity of optogenetic probes to light used to excite the sensors. Here the authors develop a Ca2+ sensor based on bioluminescence resonance energy transfer (BRET) that ...
Jie Yang +6 more
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Bioluminescence-Based Energy Transfer Using Semiconductor Quantum Dots as Acceptors
Sensors, 2020 Bioluminescence resonance energy transfer (BRET) is the non-radiative transfer of energy from a bioluminescent protein donor to a fluorophore acceptor. It shares all the formalism of Förster resonance energy transfer (FRET) but differs in one key aspect:
Anirban Samanta, Igor L. Medintz
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An endogenous green fluorescent protein–photoprotein pair in Clytia hemisphaerica eggs shows co-targeting to mitochondria and efficient bioluminescence energy transfer [PDF]
Open Biology, 2014 Green fluorescent proteins (GFPs) and calcium-activated photoproteins of the aequorin/clytin family, now widely used as research tools, were originally isolated from the hydrozoan jellyfish Aequora victoria.
Cécile Fourrage +4 more
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Bio-Protocol, 2020 G-protein coupled receptors (GPCRs) remain at the forefront of drug discovery efforts. Detailed assessment of features contributing to GPCR ligand engagement in a physiologically relevant environment is imperative to the development of new therapeutics ...
Michelle Boursier +3 more
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INVESTIGATING THE DIMERIZATION OF RASG USING BIOLUMINESCENCE RESONANCE ENERGY TRANSFER (BRET)
, 2021 In diseases such as cancer, metastasis, the spread of cancer to secondary tumor sites, is one of the greatest challenges associated with disease progression and treatment.
WEISS, ETHAN T.
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Multiple GPCR Functional Assays Based on Resonance Energy Transfer Sensors
Frontiers in Cell and Developmental Biology, 2021 G protein-coupled receptors (GPCRs) represent one of the largest membrane protein families that participate in various physiological and pathological activities.
Yiwei Zhou +5 more
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A bioluminescence resonance energy transfer (BRET) system: Application to interacting circadian clock proteins [PDF]
Proceedings of the National Academy of Sciences, 1999 We describe a method for assaying protein interactions that offers some attractive advantages over previous assays. This method, called bioluminescence resonance energy transfer (BRET), uses a bioluminescent luciferase that is genetically fused to one candidate protein, and a green fluorescent protein mutant fused to another protein of interest ...
Y, Xu, D W, Piston, C H, Johnson
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mBio, 2014 Protein-protein interactions are important for virtually every biological process, and a number of elegant approaches have been designed to detect and evaluate such interactions.
Boyu Cui +7 more
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Heterodimerization of apelin receptor and neurotensin receptor 1 induces phosphorylation of ERK1/2and cell proliferationviaGαq-mediated mechanism [PDF]
, 2014 Dimerization of G protein-coupled receptors (GPCRs) is crucial for receptor function including agonist affinity, efficacy, trafficking and specificity of signal transduction, including G protein coupling.
Cai, X +9 more
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Evolution of BRET biosensors from live cell to tissue-scale in vivo imaging
Frontiers in Endocrinology, 2013 Development of bioluminescence resonance energy transfer [BRET] based genetic sensors for sensing biological functions such as protein-protein interactions [PPIs] in vivo has a special value in measuring such dynamic events at their native environment ...
ABHIJIT eDE +3 more
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