Results 31 to 40 of about 6,388 (174)
Molecules, 2019 The bioluminescence resonance energy transfer (BRET) approach involves resonance energy transfer between a light-emitting enzyme and fluorescent acceptors.
Chayma El Khamlichi +5 more
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Scientific Reports, 2022 Bioluminescence resonance energy transfer (BRET) saturation is a method of studying protein–protein interaction (PPI) upon quantification of the dependence of the BRET signal on the acceptor/donor (A:D) expression ratio.
Benoit Besson +5 more
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Type-3 BRET, an Improved Competition-Based Bioluminescence Resonance Energy Transfer Assay [PDF]
Biophysical Journal, 2014 We show that in conventional, competition-based bioluminescence resonance energy transfer (BRET) assays of membrane protein stoichiometry, the presence of competitors can alter tagged-protein density and artifactually reduce energy transfer efficiency.
Felce, James H. +2 more
openaire +2 more sources
Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing
Nature Communications, 2016 The coupling of optogenetics with fluorescent Ca2+ sensors is confounded by sensitivity of optogenetic probes to light used to excite the sensors. Here the authors develop a Ca2+ sensor based on bioluminescence resonance energy transfer (BRET) that ...
Jie Yang +6 more
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Bioluminescence-Based Energy Transfer Using Semiconductor Quantum Dots as Acceptors
Sensors, 2020 Bioluminescence resonance energy transfer (BRET) is the non-radiative transfer of energy from a bioluminescent protein donor to a fluorophore acceptor. It shares all the formalism of Förster resonance energy transfer (FRET) but differs in one key aspect:
Anirban Samanta, Igor L. Medintz
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Bio-Protocol, 2020 G-protein coupled receptors (GPCRs) remain at the forefront of drug discovery efforts. Detailed assessment of features contributing to GPCR ligand engagement in a physiologically relevant environment is imperative to the development of new therapeutics ...
Michelle Boursier +3 more
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An endogenous green fluorescent protein–photoprotein pair in Clytia hemisphaerica eggs shows co-targeting to mitochondria and efficient bioluminescence energy transfer [PDF]
Open Biology, 2014 Green fluorescent proteins (GFPs) and calcium-activated photoproteins of the aequorin/clytin family, now widely used as research tools, were originally isolated from the hydrozoan jellyfish Aequora victoria.
Cécile Fourrage +4 more
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Frontiers in Endocrinology, 2012 The idea that seven transmembrane receptors (7TMRs; also designated G-protein coupled receptors (GPCRs)) might form dimers or higher order oligomeric complexes was formulated more than 20 years ago and has been intensively studied since then. In the last
Luka eDrinovec +3 more
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Journal of Tissue Engineering, 2021 Bioluminescent imaging (BLI) has emerged as a popular in vivo tracking modality in bone regeneration studies stemming from its clear advantages: non-invasive, real-time, and inexpensive. We recently adopted bioluminescence resonance energy transfer (BRET)
Lufei Wang +13 more
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Multiple GPCR Functional Assays Based on Resonance Energy Transfer Sensors
Frontiers in Cell and Developmental Biology, 2021 G protein-coupled receptors (GPCRs) represent one of the largest membrane protein families that participate in various physiological and pathological activities.
Yiwei Zhou +5 more
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