Results 51 to 60 of about 6,388 (174)

Novel BRET combination for detection of rapamycin-induced protein dimerization using luciferase from fungus Neonothopanus nambi

Heliyon
Bioluminescence resonance energy transfer (BRET) is one of the most promising approaches used for noninvasive imaging of protein-protein interactions in vivo. Recently, our team has discovered a genetically encodable bioluminescent system from the fungus
Aaiyas Mujawar, Shalini Dimri, Ksenia A. Palkina, Nadezhda M. Markina, Karen S. Sarkisyan, Anastasia V. Balakireva, Ilia V. Yampolsky, Abhijit De   +7 more
doaj   +1 more source

Detection of the Vascular Endothelial Growth Factor with a Novel Bioluminescence Resonance Energy Transfer Pair Using a Two-Component System

Sensors, 2017
In this paper we describe a two-component BRET (bioluminescence resonance energy transfer)-based method to detect vascular endothelial growth factor (VEGF) molecules in unknown samples as the basis for subsequent in vivo use.
Tobias Wimmer, Eva Schroeter, Birgit Lorenz, Knut Stieger   +3 more
doaj   +1 more source

Internalization dissociates β2-adrenergic receptors. [PDF]

PLoS ONE, 2011
G protein-coupled receptors (GPCRs) self-associate as dimers or higher-order oligomers in living cells. The stability of associated GPCRs has not been extensively studied, but it is generally thought that these receptors move between the plasma membrane ...
Tien-Hung Lan, Sudhakiranmayi Kuravi, Nevin A Lambert   +2 more
doaj   +1 more source

Agonist‐specific conformational dynamics at the β2‐adrenoceptor dictate allosteric modulation of Gαs signalling and bronchodilation

British Journal of Pharmacology, Volume 183, Issue 17, Page 5315-5335, September 2026.
Abstract Background and Purpose β2‐adrenoceptor (β2AR) agonists are the cornerstone of asthma therapy and promote bronchodilation through Gαs signalling in airway smooth muscle (ASM), but their efficacy is limited by β‐arrestin‐mediated β2AR desensitization.
Sushrut D. Shah, Joshua Richard, Suvankar Ghosh, Jillian Jerwick, Joice T. Gavali, Raymond B. Penn, Alexander D. MacKerell Jr, Deepak A. Deshpande   +7 more
wiley   +1 more source

Enhanced brightness of bacterial luciferase by bioluminescence resonance energy transfer

Scientific Reports, 2021
Using the lux operon (luxCDABE) of bacterial bioluminescence system as an autonomous luminous reporter has been demonstrated in bacteria, plant and mammalian cells.
Tomomi Kaku, Kazunori Sugiura, Tetsuyuki Entani, Kenji Osabe, Takeharu Nagai   +4 more
doaj   +1 more source

Recent Advances in Photocatalyst‐Driven Protein Labeling and Proximity Mapping

The Chemical Record, Volume 26, Issue 8, August 2026.
Photocatalyst‐driven protein labeling and proximity mapping have rapidly advanced as powerful strategies for spatiotemporal control in complex biological environments. This review integrates recent developments across single‐electron transfer and energy transfer‐based mechanisms and highlights how catalyst design, reactive intermediates, and diffusion ...
Shinichi Sato, Kazuki Miura, Juki Nakao, Hiroyuki Nakamura   +3 more
wiley   +1 more source

The Natural Quassinoid Brusatol Enhances Venetoclax Efficacy and Overcomes Resistance in Myeloid Leukemias

Cancer Science, Volume 117, Issue 8, Page 2274-2288, August 2026.
Schematic illustration of the mechanisms by which the natural quassinoid brusatol induces apoptosis in myeloid leukemias and enhances venetoclax efficacy via p53‐dependent apoptosis and mitochondrial dysfunction. ABSTRACT While venetoclax‐based combinations have shown promising results in acute myeloid leukemia (AML), the remission duration is ...
Shin‐ichiro Kawaguchi, Kazuya Sato, Junko Izawa, Norihito Takayama, Hiroko Hayakawa, Ken Suzaki, Susumu Goyama, Chiyoko Kato, Eiji Sakashita, Kaoru Tominaga, Hitoshi Endo, Yoshinobu Kanda   +11 more
wiley   +1 more source

NanoBRET: The Bright Future of Proximity-Based Assays

Frontiers in Bioengineering and Biotechnology, 2019
Bioluminescence resonance energy transfer (BRET) is a biophysical technique used to monitor proximity within live cells. BRET exploits the naturally occurring phenomenon of dipole-dipole energy transfer from a donor enzyme (luciferase) to an acceptor ...
Natasha C. Dale, Natasha C. Dale, Natasha C. Dale, Elizabeth K. M. Johnstone, Elizabeth K. M. Johnstone, Elizabeth K. M. Johnstone, Carl W. White, Carl W. White, Carl W. White, Kevin D. G. Pfleger, Kevin D. G. Pfleger, Kevin D. G. Pfleger, Kevin D. G. Pfleger   +12 more
doaj   +1 more source

Ratiometric Detection of Zn²⁺ Using DNAzyme-Based Bioluminescence Resonance Energy Transfer Sensors

Chemistry, 2023
While fluorescent sensors have been developed for monitoring metal ions in health and diseases, they are limited by the requirement of an excitation light source that can lead to photobleaching and a high autofluorescence background.
Yuting Wu, Whitney Lewis, Jing Luen Wai, Mengyi Xiong, Jiao Zheng, Zhenglin Yang, Chloe Gordon, Ying Lu, Siu Yee New, Xiao-Bing Zhang, Yi Lu   +10 more
doaj   +1 more source

Analysis of Protease Activity Using Quantum Dots and Resonance Energy Transfer

Theranostics, 2012
This review demonstrates the detection of protease activity based on the energy transfer of quantum dots (QDs). By incorporation of varying protease substrates into designed QD probes both in fluorescence resonance energy transfer (FRET) and ...
Gae Baik Kim, Young-Pil Kim
doaj