Results 51 to 60 of about 8,062,393 (179)

Internalization dissociates β2-adrenergic receptors. [PDF]

PLoS ONE, 2011
G protein-coupled receptors (GPCRs) self-associate as dimers or higher-order oligomers in living cells. The stability of associated GPCRs has not been extensively studied, but it is generally thought that these receptors move between the plasma membrane ...
Tien-Hung Lan, Sudhakiranmayi Kuravi, Nevin A Lambert   +2 more
doaj   +1 more source

The potential for biased signalling in the P2Y receptor family of GPCRs

British Journal of Pharmacology, EarlyView.
The purinergic receptor family is primarily activated by nucleotides, and contains members of both the G protein coupled‐receptor (GPCR) superfamily (P1 and P2Y) and ligand‐gated ion channels (P2X). The P2Y receptors are widely expressed in the human body, and given the ubiquitous nature of nucleotides, purinergic signalling is involved with a plethora
Claudia M. Sisk, Simon Pitchford, Graham Ladds   +2 more
wiley   +1 more source

Enhanced brightness of bacterial luciferase by bioluminescence resonance energy transfer

Scientific Reports, 2021
Using the lux operon (luxCDABE) of bacterial bioluminescence system as an autonomous luminous reporter has been demonstrated in bacteria, plant and mammalian cells.
Tomomi Kaku, Kazunori Sugiura, Tetsuyuki Entani, Kenji Osabe, Takeharu Nagai   +4 more
doaj   +1 more source

Systematic profiling reveals broad G protein coupling and context‐dependent modulation of cyclic AMP by the orphan receptor GPR139

British Journal of Pharmacology, EarlyView.
Background and Purpose G protein‐coupled receptors (GPCRs) are major drug targets, yet many orphan receptors remain poorly characterized. GPR139 has been implicated in CNS disorders, including schizophrenia and depression, but its signalling mechanisms remain unclear.
Boris Trapkov, Hans Bräuner, Alexander Sebastian Hauser   +2 more
wiley   +1 more source

Positive modulation of glucagon‐like peptide 1 (GLP‐1) receptor by endogenous haemorphins with implications in glucose metabolism and diabetes

British Journal of Pharmacology, EarlyView.
Haemorphins act on GLP‐1 receptor (GLP‐1R) activity and signalling in a positive allosteric manner. This leads to potentiated GLP‐1‐mediated activity and signalling, as well as insulin synthesis, which may have implications for glucose metabolism and, thereby, diabetes and obesity. (The graph was created in BioRender.com).
Farheen Badrealam Khan, Rwdah Mohamed Alameri, Yasir S. Raouf, Damien Maurel, Naiem Ahmad Wani, Anatoliy Shmygol, Irfa Anwar, Heng B. See, Elizabeth K. M. Johnstone, Elodie Dupuis, Eric Trinquet, Kevin D. G. Pfleger, Emilia Oueis, Mohammed Akli Ayoub   +13 more
wiley   +1 more source

NanoBRET: The Bright Future of Proximity-Based Assays

Frontiers in Bioengineering and Biotechnology, 2019
Bioluminescence resonance energy transfer (BRET) is a biophysical technique used to monitor proximity within live cells. BRET exploits the naturally occurring phenomenon of dipole-dipole energy transfer from a donor enzyme (luciferase) to an acceptor ...
Natasha C. Dale, Natasha C. Dale, Natasha C. Dale, Elizabeth K. M. Johnstone, Elizabeth K. M. Johnstone, Elizabeth K. M. Johnstone, Carl W. White, Carl W. White, Carl W. White, Kevin D. G. Pfleger, Kevin D. G. Pfleger, Kevin D. G. Pfleger, Kevin D. G. Pfleger   +12 more
doaj   +1 more source

Ratiometric Detection of Zn²⁺ Using DNAzyme-Based Bioluminescence Resonance Energy Transfer Sensors

Chemistry, 2023
While fluorescent sensors have been developed for monitoring metal ions in health and diseases, they are limited by the requirement of an excitation light source that can lead to photobleaching and a high autofluorescence background.
Yuting Wu, Whitney Lewis, Jing Luen Wai, Mengyi Xiong, Jiao Zheng, Zhenglin Yang, Chloe Gordon, Ying Lu, Siu Yee New, Xiao-Bing Zhang, Yi Lu   +10 more
doaj   +1 more source

Far‐field bioluminescent immune cell–mediated photodynamic therapy is limited by photon flux

Photochemistry and Photobiology, EarlyView.
Bioluminescent immune cell‐mediated photodynamic therapy uses tumor‐homing, luciferase‐expressing immune cells as internal light sources to activate photosensitizers in deep‐seated and/or micrometastatic tumors. Using both experiments and computational simulations, we find that this approach delivers insufficient far‐field energy to tumor cells to ...
Ji Tae Park, Sudip Timilsina, Rebecca C. Harman, Chi‐Yong Eom, Jeonghyun Oh, Qianqian Fang, Nozomi Nishimura, Chris B. Schaffer, Bryan Q. Spring   +8 more
wiley   +1 more source

Analysis of Protease Activity Using Quantum Dots and Resonance Energy Transfer

Theranostics, 2012
This review demonstrates the detection of protease activity based on the energy transfer of quantum dots (QDs). By incorporation of varying protease substrates into designed QD probes both in fluorescence resonance energy transfer (FRET) and ...
Gae Baik Kim, Young-Pil Kim
doaj  

Application of bioluminescence resonance energy transfer assays in primary mouse neuronal cultures

STAR Protocols
Summary: Bioluminescence resonance energy transfer (BRET) is widely employed for real-time monitoring of G protein-coupled receptor activity, interactions, and trafficking in heterologous cell lines, yet its use in neuronal systems remains limited. Here,
Hanh T.M. Hoang, Kiran George, Mohiuddin Ahmad   +2 more
doaj   +1 more source