Results 41 to 50 of about 8,062,393 (179)

Differential Water Networks Guide Selectivity Optimization of a Cell Active BPTF Inhibitor in Neuroblastoma

Angewandte Chemie International Edition, EarlyView.
BZ2 is a second‐generation Bromodomain PHD finger Transcription Factor (BPTF) inhibitor with improved selectivity over Class I and Class IV BRD off‐targets identified in our previously reported inhibitor, BZ1. Structural analyses reveal that water network engagement is key to this selectivity.
Kesavan Babu, Chun‐Ju Tsou, Sourav Das, Liying Fan, Anasuya Pal, Molly Sneddon, Timothy R. Stachowski, Priyanka Samanta, Stanley Nithianantham, Caroline Buchholz, Shi Zhang, Xiang Fu, Rahul S. Kathayat, Wenwei Lin, Yongtao Li, Lei Yang, Taosheng Chen, Marcus Fischer, Anang A. Shelat, William C. K. Pomerantz   +19 more
wiley   +1 more source

Construction of an Enzymatically-Conjugated DNA Aptamer–Protein Hybrid Molecule for Use as a BRET-Based Biosensor

Applied Sciences, 2020
DNA-protein conjugates are useful molecules for construction of biosensors. Herein, we report the development of an enzymatically-conjugated DNA aptamer–protein hybrid molecule for use as a bioluminescence resonance energy transfer (BRET)-based biosensor.
Masayasu Mie, Rena Hirashima, Yasumasa Mashimo, Eiry Kobatake   +3 more
doaj   +1 more source

Imaging protein interactions with bioluminescence resonance energy transfer (BRET) in plant and mammalian cells and tissues [PDF]

Proceedings of the National Academy of Sciences, 2007
FRET is a well established method for cellular and subcellular imaging of protein interactions. However, FRET obligatorily necessitates fluorescence excitation with its concomitant problems of photobleaching, autofluorescence, phototoxicity, and undesirable stimulation of photobiological processes.
Xiaodong, Xu, Mohammed, Soutto, Qiguang, Xie, Stein, Servick, Chitra, Subramanian, Albrecht G, von Arnim, Carl Hirschie, Johnson   +6 more
openaire   +2 more sources

Differential effects of glucose‐dependent insulinotropic polypeptide receptor/glucagon‐like peptide‐1 receptor heteromerization on cell signaling when expressed in HEK‐293 cells

Pharmacology Research & Perspectives, 2022
The incretin hormones: glucose‐dependent insulinotropic polypeptide (GIP) and glucagon‐like peptide‐1 (GLP‐1) are important regulators of many aspects of metabolism including insulin secretion.
Bashaier Al‐Zaid, Siby Chacko, Charles Ifeamalume Ezeamuzie, Moritz Bünemann, Cornelius Krasel, Tina Karimian, Peter Lanzerstorfer, Suleiman Al‐Sabah   +7 more
doaj   +1 more source

Multiplexing of Multicolor Bioluminescence Resonance Energy Transfer [PDF]

, 2010
Bioluminescence resonance energy transfer (BRET) is increasingly being used to monitor protein-protein interactions and cellular events in cells. However, the ability to monitor multiple events simultaneously is limited by the spectral properties of the ...
Le Gouill, Christian, Bouvier, Michel, Christian Le Gouill, Breton, Billy, Bonin, Hélène, Joris Zhou, Zhou, Joris, Michel Bouvier, Sauvageau, Étienne, Billy Breton, Étienne Sauvageau, Hélène Bonin   +11 more
core   +1 more source

Progress report on new epilepsy treatments: A summary of the Eighteenth Eilat Conference on New Antiepileptic Drugs and Devices (EILAT XVIII). II. Treatments in more advanced clinical development

Epilepsia, EarlyView.
Abstract This article summarizes data for 13 investigational treatments for which at least preliminary seizure outcome data in patients with epilepsy were reported at the Eighteenth Eilat Conference on New Antiepileptic Drugs and Devices held in Madrid, Spain, on May 3–6, 2026.
Meir Bialer, Cecilie Johannessen Landmark, Matthias J. Koepp, Emilio Perucca, Piero Perucca, Torbjörn Tomson, H. Steve White, Elaine Wirrell   +7 more
wiley   +1 more source

Activation of peripheral NOP receptors reduces periorbital mechanical allodynia evoked by CGRP in mice

British Journal of Pharmacology, EarlyView.
Background and Purpose Migraine is a neurovascular disorder largely mediated by calcitonin gene‐related peptide (CGRP). This study explores the role of the nociceptin/orphanin FQ (N/OFQ)–N/OFQ receptor (NOP) system in CGRP‐induced periorbital mechanical allodynia (PMA) in mice.
Chiara Sturaro, Pietro Pola, Michela Argentieri, Alessia Frezza, Matilde Marini, Francesco De Logu, Valentina Albanese, Marie Soukupova, Davide Malfacini, Nurulain T. Zaveri, Romina Nassini, Dane D. Jensen, Pierangelo Geppetti, Girolamo Calò, Chiara Ruzza   +14 more
wiley   +1 more source

Thermostable Bioluminescent Intercalating Dyes for Real‐Time, Integrated Nucleic Acid Amplification and Detection

Angewandte Chemie, Volume 138, Issue 29, 13 July 2026.
We present a bioluminescent diagnostic platform that integrates DNA amplification and detection in a single step. The engineering of thermostable luciferase‐intercalating dye conjugates and controlled release of caged luciferin substrates enable real‐time, detection of attomolar concentrations of viral DNA within 30 min.
Yosta de Stigter, Harmen J. van der Veer, Sterre de Lignie, Robbert J. de Haas, Renko de Vries, Joost P. H. Schoeber, Anne J. M. Loonen, Adriaan J. C. van den Brule, Maarten Merkx   +8 more
wiley   +2 more sources

Novel BRET combination for detection of rapamycin-induced protein dimerization using luciferase from fungus Neonothopanus nambi

Heliyon
Bioluminescence resonance energy transfer (BRET) is one of the most promising approaches used for noninvasive imaging of protein-protein interactions in vivo. Recently, our team has discovered a genetically encodable bioluminescent system from the fungus
Aaiyas Mujawar, Shalini Dimri, Ksenia A. Palkina, Nadezhda M. Markina, Karen S. Sarkisyan, Anastasia V. Balakireva, Ilia V. Yampolsky, Abhijit De   +7 more
doaj   +1 more source

Detection of the Vascular Endothelial Growth Factor with a Novel Bioluminescence Resonance Energy Transfer Pair Using a Two-Component System

Sensors, 2017
In this paper we describe a two-component BRET (bioluminescence resonance energy transfer)-based method to detect vascular endothelial growth factor (VEGF) molecules in unknown samples as the basis for subsequent in vivo use.
Tobias Wimmer, Eva Schroeter, Birgit Lorenz, Knut Stieger   +3 more
doaj   +1 more source