Results 41 to 50 of about 5,054 (183)

Equilibrium and Kinetic Measurements of Ligand Binding to HiBiT-tagged GPCRs on the Surface of Living Cells

Bio-Protocol, 2020
G-protein coupled receptors (GPCRs) remain at the forefront of drug discovery efforts. Detailed assessment of features contributing to GPCR ligand engagement in a physiologically relevant environment is imperative to the development of new therapeutics ...
Michelle Boursier, Sergiy Levin, Robin Hurst, Rachel Friedman Ohana   +3 more
doaj   +1 more source

Heterodimerization of apelin receptor and neurotensin receptor 1 induces phosphorylation of ERK1/2and cell proliferationviaGαq-mediated mechanism [PDF]

, 2014
Dimerization of G protein-coupled receptors (GPCRs) is crucial for receptor function including agonist affinity, efficacy, trafficking and specificity of signal transduction, including G protein coupling.
Cai, X, Jiang, Y, Karteris, E, Bai, B, Chen, J, Karteris, Emmanouil, Bai, Bo, Jiang, Yunlu, Cai, Xin, Chen, Jing   +9 more
core   +1 more source

Multiple GPCR Functional Assays Based on Resonance Energy Transfer Sensors

Frontiers in Cell and Developmental Biology, 2021
G protein-coupled receptors (GPCRs) represent one of the largest membrane protein families that participate in various physiological and pathological activities.
Yiwei Zhou, Jiyong Meng, Chanjuan Xu, Chanjuan Xu, Jianfeng Liu, Jianfeng Liu   +5 more
doaj   +1 more source

Bioluminescence Resonance Energy Transfer System for Measuring Dynamic Protein-Protein Interactions in Bacteria

mBio, 2014
Protein-protein interactions are important for virtually every biological process, and a number of elegant approaches have been designed to detect and evaluate such interactions.
Boyu Cui, Yao Wang, Yunhong Song, Tietao Wang, Changfu Li, Yahong Wei, Zhao-Qing Luo, Xihui Shen   +7 more
doaj   +1 more source

Bioluminescence Resonance Energy Transfer Reveals the Adrenocorticotropin (ACTH)-Induced Conformational Change of the Activated ACTH Receptor Complex in Living Cells

, 2011
Bioluminescence resonance energy transfer analysis is used to study the interaction between melanocortin 2 receptor (MC2R) accessory protein and the MC2R and provides evidence for protein kinase A-dependent conformational changes in the receptor complex ...
Adrian J. L. Clark, Szidonya, L, Laszlo Szidonya, Cooray, SN, Mazhar, K, Teng-Teng Chung, Clark, AJ, Khansa Mazhar, Chung, TT, Sadani N. Cooray   +9 more
core   +1 more source

Evolution of BRET biosensors from live cell to tissue-scale in vivo imaging

Frontiers in Endocrinology, 2013
Development of bioluminescence resonance energy transfer [BRET] based genetic sensors for sensing biological functions such as protein-protein interactions [PPIs] in vivo has a special value in measuring such dynamic events at their native environment ...
ABHIJIT eDE, Akshi eJasani, Rohit eArora, Sanjiv Sam Gambhir   +3 more
doaj   +1 more source

Imaging protein interactions with bioluminescence resonance energy transfer (BRET) in plant and mammalian cells and tissues [PDF]

Proceedings of the National Academy of Sciences, 2007
FRET is a well established method for cellular and subcellular imaging of protein interactions. However, FRET obligatorily necessitates fluorescence excitation with its concomitant problems of photobleaching, autofluorescence, phototoxicity, and undesirable stimulation of photobiological processes.
Xiaodong, Xu, Mohammed, Soutto, Qiguang, Xie, Stein, Servick, Chitra, Subramanian, Albrecht G, von Arnim, Carl Hirschie, Johnson   +6 more
openaire   +2 more sources

Construction of an Enzymatically-Conjugated DNA Aptamer–Protein Hybrid Molecule for Use as a BRET-Based Biosensor

Applied Sciences, 2020
DNA-protein conjugates are useful molecules for construction of biosensors. Herein, we report the development of an enzymatically-conjugated DNA aptamer–protein hybrid molecule for use as a bioluminescence resonance energy transfer (BRET)-based biosensor.
Masayasu Mie, Rena Hirashima, Yasumasa Mashimo, Eiry Kobatake   +3 more
doaj   +1 more source

Differential effects of glucose‐dependent insulinotropic polypeptide receptor/glucagon‐like peptide‐1 receptor heteromerization on cell signaling when expressed in HEK‐293 cells

Pharmacology Research & Perspectives, 2022
The incretin hormones: glucose‐dependent insulinotropic polypeptide (GIP) and glucagon‐like peptide‐1 (GLP‐1) are important regulators of many aspects of metabolism including insulin secretion.
Bashaier Al‐Zaid, Siby Chacko, Charles Ifeamalume Ezeamuzie, Moritz Bünemann, Cornelius Krasel, Tina Karimian, Peter Lanzerstorfer, Suleiman Al‐Sabah   +7 more
doaj   +1 more source

Bioluminescence Resonance Energy Transfer (BRET) to Detect the Interactions Between Kappa Opioid Receptor and Nonvisual Arrestins

, 2020
Bioluminescence resonance energy transfer (BRET) is a very sensitive technique employed to study protein–protein interactions, including G-protein-coupled receptor (GPCR) hetero- and homo-dimerization. Recently, BRET has also been used to investigate the
Andrea Bedini, Bedini A.
core   +1 more source