Results 71 to 80 of about 6,388 (174)

tracerDB: a crowdsourced fluorescent tracer database for target engagement analysis

Nature Communications
Investigating ligand-protein complexes is essential in the areas of chemical biology and drug discovery. However, detailed information on key reagents such as fluorescent tracers and associated data for the development of widely used bioluminescence ...
Johannes Dopfer, James D. Vasta, Susanne Müller, Stefan Knapp, Matthew B. Robers, Martin P. Schwalm   +5 more
doaj   +1 more source

Microbial Metabolite‐Stimulated Bitter Taste Receptor T2R14 Signaling Is Modulated by CFTR Interactions

The FASEB Journal, Volume 40, Issue 11, 15 June 2026.
Bitter taste receptors (T2Rs) play an important role in physiological and cellular responses. Bitter tasting chemicals and microbial metabolites can bind to T2R14, which enables interactions between T2R14 and specific intracellular domains of the cystic fibrosis transmembrane conductance regulator (CFTR) anion channel.
Tejas Gupte, Nisha Singh, Vikram Bhatia, Kavisha Arora, Shayan Amiri, Paul Fernyhough, Anjaparavanda P. Naren, Shyamala Dakshinamurti, Prashen Chelikani   +8 more
wiley   +1 more source

Biophysical detection of diversity and bias in GPCR function

Frontiers in Endocrinology, 2014
G protein-coupled receptors (GPCRs) function in complexes with a range of molecules and proteins including ligands, G proteins, arrestins, ubiquitin and other receptors.
Werner eJaeger, Stephen eArmstrong, Stephen eHill, Kevin D G Pfleger, Kevin D G Pfleger   +4 more
doaj   +1 more source

Using a NanoBRET‐Based Ligand‐Binding Assay at the β2‐Adrenoceptor for Undergraduate Pharmacology Education

Pharmacology Research &Perspectives, Volume 14, Issue 3, June 2026.
ABSTRACT The teaching of pharmacology includes a fundamental understanding of ligand binding; how it is measured and how it is calculated. We sought to modernize the techniques by which ligand affinity is determined by undergraduate students and introduce them to parameter estimation through curve fitting.
Abigail Pearce, Xianglin Huang, Theo Redfern‐Nichols, Claudia M. Sisk, Dewi Safitri, Michael Collins, Milena A. Malcharek, Sergio Tomey García, Chiharu Nakamura, Andrzej Harris, Graham Ladds   +10 more
wiley   +1 more source

NanoBRET in C. elegans illuminates functional receptor interactions in real time

BMC Molecular and Cell Biology, 2022
Background Protein-protein interactions form the basis of every organism and thus, investigating their dynamics, intracellular protein localization, trafficking and interactions of distinct proteins such as receptors and their ligand-binding are of ...
Victoria Elisabeth Groß, Miron Mikhailowitsch Gershkovich, Torsten Schöneberg, Anette Kaiser, Simone Prömel   +4 more
doaj   +1 more source

Comprehensive transduceromic profiling of NOP receptor ligands at different Gα subunits

British Journal of Pharmacology, Volume 183, Issue 12, Page 3195-3212, June 2026.
Abstract Background and Purpose The nociceptin/orphanin FQ (N/OFQ) peptide receptor (NOP) is an opioid receptor family member with distinct pharmacology and broad therapeutic potential. NOP receptors regulate functions such as nociception, stress reactivity, reward, mood, autonomic tone, immunity and sleep/wake cycle.
Davide Malfacini, Riccardo Camilotto, Michele Maritan, Erika Morrone, Stephen Afriyie Boateng, Anna Vacilotto, Salvatore Pacifico, Remo Guerrini, Nurulain T. Zaveri, Girolamo Calo'   +9 more
wiley   +1 more source

Light‐ and Bioluminescence‐Controlled Reversible Protein Conjugation on the Inner Leaflet of Asymmetric Phospholipid‐Amphiphilic Protein Hybrid Vesicles

ChemPlusChem, Volume 91, Issue 5, May 2026.
We established light‐ and bioluminescence‐responsive systems that allow reversible conjugation and dissociation of proteins on the inner leaflet of asymmetric lipid–amphiphilic protein hybrid vesicles. These systems provide a versatile platform for precise spatiotemporal regulation and bioluminescence‐triggered protein release within synthetic vesicles.
Masato Suzuki, Koki Kamiya
wiley   +1 more source

Real‐Life Application of a Point‐of‐Care Biosensor for Phenylalanine in Patients With Phenylketonuria

Journal of Inherited Metabolic Disease, Volume 49, Issue 3, May 2026.
ABSTRACT Phenylketonuria (PKU) is a rare metabolic disorder causing elevated phenylalanine (PHE) levels requiring lifelong dietary or pharmacological management and regular monitoring. Current PHE monitoring methods, such as tandem mass spectrometry (FIA‐MS/MS), are laboratory and sample transport‐dependent, leading to delays in obtaining results. This
Corentin Gondrand, Anna T. Reischl‐Hajiabadi, Estelle Bonedeau, Nicolas Weiß, Gerd Schneider, Maurice Fahn, Frederic Myers, Philipp Minel Städle, Kathrin Jeltsch, Georg F. Hoffmann, Jürgen G. Okun, Dorothea Haas, Sven F. Garbade, Kai Johnsson, Thomas Opladen   +14 more
wiley   +1 more source

Profiling of HCAR1 signalling reveals Gαi/o and Gαs activation without β‐arrestin recruitment and the discovery of an allosteric agonist

British Journal of Pharmacology, Volume 183, Issue 10, Page 2219-2235, May 2026.
Abstract Background and Purpose Lactate, historically viewed as a metabolic by‐product, has emerged as a signalling molecule via the G protein–coupled receptor Hydroxycarboxylic Acid Receptor 1 (HCAR1). The receptor is primarily expressed in adipocytes but also found in various other tissues.
Simon Lind, Shane C. Wright, Emilia Gvozdenovic, Kristina Nilsson, Kenneth L. Granberg, Michel Bouvier, Linda C. Johansson   +6 more
wiley   +1 more source

Greatly enhanced detection of a volatile ligand at femtomolar levels using bioluminescence resonance energy transfer (BRET).

Biosensors & bioelectronics, 2011
Our goal is to develop a general transduction system for G-protein coupled receptors (GPCRs). GPCRs are present in most eukaryote cells and transduce diverse extracellular signals.
H. Dacres, Jian Wang, V. Leitch, I. Horne, A. Anderson, S. Trowell   +5 more
semanticscholar   +1 more source