Results 81 to 90 of about 6,388 (174)

Monitoring Opioid Receptor Dimerization in Living Cells by Bioluminescence Resonance Energy Transfer (BRET)

, 2014
Bioluminescence resonance energy transfer (BRET) is a natural phenomenon that has been successfully applied for the study of protein-protein interactions, including opioid receptor oligomers. The discovery of opioid receptor homomers and heteromers has brought to the finding of new functions and new way of signaling and trafficking; therefore, opioid ...
openaire   +3 more sources

pHlash: a new genetically encoded and ratiometric luminescence sensor of intracellular pH.

PLoS ONE, 2012
We report the development of a genetically encodable and ratiometic pH probe named "pHlash" that utilizes Bioluminescence Resonance Energy Transfer (BRET) rather than fluorescence excitation.
Yunfei Zhang, Qiguang Xie, J Brian Robertson, Carl Hirschie Johnson   +3 more
doaj   +1 more source

Enhanced BRET technology for the monitoring of agonist-induced and agonist-independent interactions between GPCRs and β-arrestins

Frontiers in Endocrinology, 2011
The bioluminescence resonance energy transfer (BRET) technique has become extremely valuable for the real-time monitoring of protein-protein interactions in live cells.
Martina eKocan, Martina eKocan, Matthew eDalrymple, Ruth eSeeber, Brian eFeldman, Kevin ePfleger   +5 more
doaj   +1 more source

In Vivo Biosensing Using Resonance Energy Transfer

Biosensors, 2019
Solution-phase and intracellular biosensing has substantially enhanced our understanding of molecular processes foundational to biology and pathology. Optical methods are favored because of the low cost of probes and instrumentation.
Shashi Bhuckory, Joshua C. Kays, Allison M. Dennis   +2 more
doaj   +1 more source

A bioluminescence resonance energy transfer (BRET) assay to detect telomere length in S. cerevisiae

bioRxiv
A method to measure telomere length in S. cerevisiae was developed based on bioluminescence resonance energy transfer (BRET). The system uses energy transfer between a luciferase-Rif2 fusion protein and fluorescently tagged Rap1.
Felix Richter, Honorata M. Ropiak, Jörg Urban, J. Franke   +3 more
semanticscholar   +1 more source

Optimized in vivo multispectral bioluminescent imaging of tumor biology using engineered BRET reporters

iScience
Summary: The ability to visualize and track multiple biological processes in vivo in real time is highly desirable. Bioluminescence imaging (BLI) has emerged as an attractive modality for non-invasive cell tracking, with various luciferase reporters ...
Bryan Labra, Kshitij Parag-Sharma, John J. Powers, Sonal Srivastava, Joel R. Walker, Thomas A. Kirkland, Caroline K. Brennan, Jennifer A. Prescher, Antonio L. Amelio   +8 more
doaj   +1 more source

ATP- and Adenosine-Mediated Signaling in the Central Nervous System: Purinergic Receptor Complex: Generating Adenine Nucleotide-Sensitive Adenosine Receptors

Journal of Pharmacological Sciences, 2004
Adenosine A1 receptors (A1R) are able to form a heteromeric complex with P2Y1 receptors (P2Y1R) that generates A1R with P2Y1R-like agonistic pharmacology.
Kazuaki Yoshioka, Hiroyasu Nakata
doaj   +1 more source

Setting up a Bioluminescence Resonance Energy Transfer high throughput screening assay to search for protein/protein interaction inhibitors in mammalian cells.

Frontiers in Endocrinology, 2012
Each step of the cell life and its response or adaptation to its environment are mediated by a network of protein/protein interactions termed interactome.
Cyril eCouturier, Benoit eDeprez
doaj   +1 more source

Application of FRET- and BRET-based live-cell biosensors in deorphanization and ligand discovery studies on orphan G protein-coupled receptors

SLAS Discovery
Bioluminescence- and fluorescence-based resonance energy transfer assays have gained considerable attention in pharmacological research as high-throughput scalable tools applicable to drug discovery. To this end, G protein-coupled receptors represent the
Joanna J. Sajkowska, Choi Har Tsang, Paweł Kozielewicz   +2 more
doaj   +1 more source

Detection of beta 2-adrenergic receptor dimerization in living cells using bioluminescence resonance energy transfer (BRET).

Proceedings of the National Academy of Sciences of the United States of America, 2000
S. Angers, A. Salahpour, E. Joly, S. Hilairet, D. Chelsky, M. Dennis, M. Bouvier   +6 more
semanticscholar   +1 more source