Results 81 to 90 of about 5,054 (183)

Enhanced Molecular Tension Sensor Based on Bioluminescence Resonance Energy Transfer (BRET)

, 2019
Molecular tension sensors measure piconewton forces experienced by individual proteins in the context of the cellular microenvironment. Current genetically encoded tension sensors use FRET to report on extension of a deformable peptide encoded in a ...
Wendy R. Gordon (8162211), Maria Paz Ramirez, Kassidy J. Tompkins (8261694), Maria Paz Ramirez (8261697), Kassidy J. Tompkins, Eric J. Aird, Wendy R. Gordon, Eric J. Aird (8162208)   +7 more
core   +1 more source

GPCR-OKB: the G protein coupled receptor oligomer knowledge base [PDF]

, 2010
Rapid expansion of available data about G Protein Coupled Receptor (GPCR) dimers/oligomers over the past few years requires an effective system to organize this information electronically.
Bas Vroling, Pin, J.-P., Palczewski, K., Camacho-Artacho, Marta, Pin, J.P., Lucy Skrabanek, Jean-Philippe Pin, Marta Camacho-Artacho, Devi, Lakshmi A, Graeme Milligan, Marta Filizola, Campagne, Fabien, Fabien Campagne, Martin J. Lohse, Jufang Shan, Lohse, Martin J, Vriend, Gerrit, Khelashvili, George, Milligan, Graeme, Camacho-Artacho, M., Skrabanek, L., George Khelashvili, Campagne, F., Susan R. George, Lakshmi A. Devi, Filizola, M., Pin, Jean-Philippe, Vroling, B., Bouvier, M., Devi, L.A., Richard R. Neubig, Marc Parmentier, George, Susan R, Palczewski, Krzysztof, Dorff, K., Shan, J., Vriend, G., Michel Bouvier, Lohse, M.J., Bouvier, Michel, Skrabanek, Lucy, Vroling, Bas, Filizola, Marta, Neubig, Richard R, Gerrit Vriend, Dorff, Kevin, Parmentier, Marc, Khelashvili, G., Javitch, J.A., Shan, Jufang, Parmentier, M., Javitch, Jonathan A, Krzysztof Palczewski, Kevin Dorff, Jonathan A. Javitch, George, S.R., Neubig, R.R., Milligan, G.   +57 more
core   +1 more source

Profiling of HCAR1 signalling reveals Gαi/o and Gαs activation without β‐arrestin recruitment and the discovery of an allosteric agonist

British Journal of Pharmacology, Volume 183, Issue 10, Page 2219-2235, May 2026.
Abstract Background and Purpose Lactate, historically viewed as a metabolic by‐product, has emerged as a signalling molecule via the G protein–coupled receptor Hydroxycarboxylic Acid Receptor 1 (HCAR1). The receptor is primarily expressed in adipocytes but also found in various other tissues.
Simon Lind, Shane C. Wright, Emilia Gvozdenovic, Kristina Nilsson, Kenneth L. Granberg, Michel Bouvier, Linda C. Johansson   +6 more
wiley   +1 more source

Multiplexing of Multicolor Bioluminescence Resonance Energy Transfer

, 2010
Bioluminescence resonance energy transfer (BRET) is increasingly being used to monitor protein-protein interactions and cellular events in cells. However, the ability to monitor multiple events simultaneously is limited by the spectral properties of the ...
Le Gouill, Christian, Bouvier, Michel, Christian Le Gouill, Breton, Billy, Bonin, Hélène, Joris Zhou, Zhou, Joris, Michel Bouvier, Sauvageau, Étienne, Billy Breton, Étienne Sauvageau, Hélène Bonin   +11 more
core   +1 more source

Confirmation of SGIp1: endophilin interaction using bioluminescence resonance energy transfer (BRET)

, 2004
Confirmation of SGIp1: endophilin interaction using bioluminescence resonance energy transfer ...
Kristy Bolton (13077711), Gregory Collier (13049697), Matthew Prior (13055622), Richard Fahey (13049820), L Kerr-Bayles (13281264), Ken Walder (13099344), J Trevaskis (13280241), K Whitecross (13279590)   +7 more
core  

Genetically Encoded RNA-Based Bioluminescence Resonance Energy Transfer (BRET) Sensors

RNA-based nanostructures and molecular devices have become popular for developing biosensors and genetic regulators. These programmable RNA nanodevices can be genetically encoded and modularly engineered to detect various cellular targets and then induce
Lan Mi (813938), Zhining Sun (8468970), Aruni P. K. K. Karunanayake Mudiyanselage (8374083), Rigumula Wu (4671865), Kewei Ren (1770106), Qikun Yu (5491733), Mingxu You (1312845), Ru Zheng (1402009)   +7 more
core   +1 more source

Functional divergence of protein kinase A regulatory subunit Iβ variants: the importance of N3A motifs in PKA regulation

The FEBS Journal, Volume 293, Issue 8, Page 2417-2434, April 2026.
The regulatory subunit RIβ of protein kinase A occurs in two variants that differ at residue 268 (A268 versus R268). This seemingly small substitution significantly changes cAMP sensitivity, basal kinase activity, and the dynamic behavior of the regulatory domains.
Maximilian Wallbott, Jui‐Hung Weng, Valeria Pane, Yuliang Ma, Jian Wu, Susan S. Taylor, Friedrich W. Herberg   +6 more
wiley   +1 more source

Attenuated Bacteria‐Based Tumor Therapy: Clinical Application Risks, Marketing Approval Restrictions, and Coping Strategies

Advanced Science, Volume 13, Issue 16, 18 March 2026.
This review comprehensively overviews attenuated bacteria‐based tumor therapy, highlighting key methodologies and recent progress. It examines strategies for enhancing safety, stabilizing efficacy, optimizing manufacturing, and navigating regulatory pathways.
Yucheng Liu, Junhong Yao, Jiaxin Deng, Minjie Zhou, Kaiyue Shen, Shishuo Sun, Xiaoge Gao, Qing Zhang, Haiheng Xu   +8 more
wiley   +1 more source

pHlash: a new genetically encoded and ratiometric luminescence sensor of intracellular pH.

PLoS ONE, 2012
We report the development of a genetically encodable and ratiometic pH probe named "pHlash" that utilizes Bioluminescence Resonance Energy Transfer (BRET) rather than fluorescence excitation.
Yunfei Zhang, Qiguang Xie, J Brian Robertson, Carl Hirschie Johnson   +3 more
doaj   +1 more source

A rigorous experimental framework for detecting protein oligomerization using bioluminescence resonance energy transfer.

, 2006
Bioluminescence resonance energy transfer (BRET), which relies on nonradiative energy transfer between luciferase-coupled donors and GFP-coupled acceptors, is emerging as a useful tool for analyzing the quaternary structures of cell-surface molecules ...
John R James, Davis, Simon J, James, JR, Carmo, AM, Davis, Simon, Marta I Oliveira, Iaboni, A, James, John R., Oliveira, Marta I, Simon J Davis, Iaboni, Andrea, Alexandre M Carmo, Davis, SJ, Oliveira, MI, Carmo, Alexandre M, Andrea Iaboni   +15 more
core   +1 more source