Results 81 to 90 of about 8,062,393 (179)

A Constellation of Fluorescent Biosensors to Illuminate the Galaxy of Protein Kinases

ChemBioChem, Volume 27, Issue 11, 15 June 2026.
Protein kinases (PKs) are enzymes that catalyze phosphorylation of protein substrates involved in a wide variety of biological signalling pathways. This review describes the different families and mechanisms of action and regulation of protein kinases, together with the different classes of fluorescent biosensors that have been engineered and ...
Timothe Abbura, May C. Morris
wiley   +1 more source

Bioluminescence resonance energy transfer (BRET) imaging of protein–protein interactions within deep tissues of living subjects

, 2011
Identifying protein–protein interactions (PPIs) is essential for understanding various disease mechanisms and developing new therapeutic approaches.
Carmel T. Chan, Anca Dragulescu-Andrasi, Abhijit De, Tarik F. Massoud, Sanjiv S. Gambhir   +4 more
core   +1 more source

Microbial Metabolite‐Stimulated Bitter Taste Receptor T2R14 Signaling Is Modulated by CFTR Interactions

The FASEB Journal, Volume 40, Issue 11, 15 June 2026.
Bitter taste receptors (T2Rs) play an important role in physiological and cellular responses. Bitter tasting chemicals and microbial metabolites can bind to T2R14, which enables interactions between T2R14 and specific intracellular domains of the cystic fibrosis transmembrane conductance regulator (CFTR) anion channel.
Tejas Gupte, Nisha Singh, Vikram Bhatia, Kavisha Arora, Shayan Amiri, Paul Fernyhough, Anjaparavanda P. Naren, Shyamala Dakshinamurti, Prashen Chelikani   +8 more
wiley   +1 more source

Bioluminescence Resonance Energy Transfer: A Powerful Technique in Neuropharmacology for Discovering New Ligands of G Protein-Coupled Receptors

MasterThe Bioluminescence Resonance Energy Transfer (BRET) is a proximity-based assay used to study protein-protein interactions in living cells. The principle behind BRET involves resonance transfer of energy between bioluminescent enzymes and a ...
Morisset-Lopez, Séverine
core   +4 more sources

pHlash: a new genetically encoded and ratiometric luminescence sensor of intracellular pH.

PLoS ONE, 2012
We report the development of a genetically encodable and ratiometic pH probe named "pHlash" that utilizes Bioluminescence Resonance Energy Transfer (BRET) rather than fluorescence excitation.
Yunfei Zhang, Qiguang Xie, J Brian Robertson, Carl Hirschie Johnson   +3 more
doaj   +1 more source

Fluorescence resonance energy transfer studies of protein interactions [PDF]

, 2008
This thesis presents an investigation of fluorescence resonance energy transfer (FRET) as a reporting signal for protein-protein interactions. Quantitative optical assays to measure protein binding, conjugation and deconjugation are developed and results
Martin, Sarah Friede
core   +2 more sources

Enhanced BRET technology for the monitoring of agonist-induced and agonist-independent interactions between GPCRs and β-arrestins

Frontiers in Endocrinology, 2011
The bioluminescence resonance energy transfer (BRET) technique has become extremely valuable for the real-time monitoring of protein-protein interactions in live cells.
Martina eKocan, Martina eKocan, Matthew eDalrymple, Ruth eSeeber, Brian eFeldman, Kevin ePfleger   +5 more
doaj   +1 more source

In Vivo Biosensing Using Resonance Energy Transfer

Biosensors, 2019
Solution-phase and intracellular biosensing has substantially enhanced our understanding of molecular processes foundational to biology and pathology. Optical methods are favored because of the low cost of probes and instrumentation.
Shashi Bhuckory, Joshua C. Kays, Allison M. Dennis   +2 more
doaj   +1 more source

Optimized in vivo multispectral bioluminescent imaging of tumor biology using engineered BRET reporters

iScience
Summary: The ability to visualize and track multiple biological processes in vivo in real time is highly desirable. Bioluminescence imaging (BLI) has emerged as an attractive modality for non-invasive cell tracking, with various luciferase reporters ...
Bryan Labra, Kshitij Parag-Sharma, John J. Powers, Sonal Srivastava, Joel R. Walker, Thomas A. Kirkland, Caroline K. Brennan, Jennifer A. Prescher, Antonio L. Amelio   +8 more
doaj   +1 more source

A rigorous experimental framework for detecting protein oligomerization using bioluminescence resonance energy transfer.

, 2006
Bioluminescence resonance energy transfer (BRET), which relies on nonradiative energy transfer between luciferase-coupled donors and GFP-coupled acceptors, is emerging as a useful tool for analyzing the quaternary structures of cell-surface molecules ...
John R James, Davis, Simon J, James, JR, Carmo, AM, Davis, Simon, Marta I Oliveira, Iaboni, A, James, John R., Oliveira, Marta I, Simon J Davis, Iaboni, Andrea, Alexandre M Carmo, Davis, SJ, Oliveira, MI, Carmo, Alexandre M, Andrea Iaboni   +15 more
core   +1 more source