Results 91 to 100 of about 5,054 (183)

Enhanced BRET technology for the monitoring of agonist-induced and agonist-independent interactions between GPCRs and β-arrestins

Frontiers in Endocrinology, 2011
The bioluminescence resonance energy transfer (BRET) technique has become extremely valuable for the real-time monitoring of protein-protein interactions in live cells.
Martina eKocan, Martina eKocan, Matthew eDalrymple, Ruth eSeeber, Brian eFeldman, Kevin ePfleger   +5 more
doaj   +1 more source

INVESTIGATING THE DIMERIZATION OF RASG USING BIOLUMINESCENCE RESONANCE ENERGY TRANSFER (BRET)

, 2021
In diseases such as cancer, metastasis, the spread of cancer to secondary tumor sites, is one of the greatest challenges associated with disease progression and treatment.
WEISS, ETHAN T.
core  

In Vivo Biosensing Using Resonance Energy Transfer

Biosensors, 2019
Solution-phase and intracellular biosensing has substantially enhanced our understanding of molecular processes foundational to biology and pathology. Optical methods are favored because of the low cost of probes and instrumentation.
Shashi Bhuckory, Joshua C. Kays, Allison M. Dennis   +2 more
doaj   +1 more source

ATP- and Adenosine-Mediated Signaling in the Central Nervous System: Purinergic Receptor Complex: Generating Adenine Nucleotide-Sensitive Adenosine Receptors

Journal of Pharmacological Sciences, 2004
Adenosine A1 receptors (A1R) are able to form a heteromeric complex with P2Y1 receptors (P2Y1R) that generates A1R with P2Y1R-like agonistic pharmacology.
Kazuaki Yoshioka, Hiroyasu Nakata
doaj   +1 more source

Optimized in vivo multispectral bioluminescent imaging of tumor biology using engineered BRET reporters

iScience
Summary: The ability to visualize and track multiple biological processes in vivo in real time is highly desirable. Bioluminescence imaging (BLI) has emerged as an attractive modality for non-invasive cell tracking, with various luciferase reporters ...
Bryan Labra, Kshitij Parag-Sharma, John J. Powers, Sonal Srivastava, Joel R. Walker, Thomas A. Kirkland, Caroline K. Brennan, Jennifer A. Prescher, Antonio L. Amelio   +8 more
doaj   +1 more source

Greatly enhanced detection of a volatile ligand at femtomolar levels using bioluminescence resonance energy transfer (BRET)

Biosensors and Bioelectronics, 2011
Our goal is to develop a general transduction system for G-protein coupled receptors (GPCRs). GPCRs are present in most eukaryote cells and transduce diverse extracellular signals. GPCRs comprise not only the largest class of integral membrane receptors but also the largest class of targets for therapeutic drugs. In all cases studied, binding of ligand
Dacres, Helen, Wang, Jian, Leitch, Virginia, Horne, Irene, Anderson, Alisha R., Trowell, Stephen C.   +5 more
openaire   +2 more sources

Predicting response to platinum and non-platinum drugs through bioluminescence resonance energy transfer (BRET) based bio-molecular interactions in platinum resistant epithelial ovarian cancer. [PDF]

Transl Oncol, 2021
Bishnu A, Mehrotra M, Dhadve A, Dimri S, De A, Ray P.   +5 more
europepmc   +1 more source

Bioluminescence Imaging of Inflammation in Vivo Based on Bioluminescence and Fluorescence Resonance Energy Transfer Using Nanobubble Ultrasound Contrast Agent

, 2019
Inflammation is an immunological response involved in various inflammatory disorders ranging from neurodegenerative diseases to cancers. Luminol has been reported to detect myeloperoxidase (MPO) activity in an inflamed area through a light-emitting ...
Yunxue Xu, Zhifei Dai (1515892), Jie Tang (46802), Jie Tang, Renfa Liu, Renfa Liu (1515895), Zhifei Dai, Yunxue Xu (4388278)   +7 more
core   +1 more source

Setting up a Bioluminescence Resonance Energy Transfer high throughput screening assay to search for protein/protein interaction inhibitors in mammalian cells.

Frontiers in Endocrinology, 2012
Each step of the cell life and its response or adaptation to its environment are mediated by a network of protein/protein interactions termed interactome.
Cyril eCouturier, Benoit eDeprez
doaj   +1 more source

Application of FRET- and BRET-based live-cell biosensors in deorphanization and ligand discovery studies on orphan G protein-coupled receptors

SLAS Discovery
Bioluminescence- and fluorescence-based resonance energy transfer assays have gained considerable attention in pharmacological research as high-throughput scalable tools applicable to drug discovery. To this end, G protein-coupled receptors represent the
Joanna J. Sajkowska, Choi Har Tsang, Paweł Kozielewicz   +2 more
doaj   +1 more source