Results 101 to 110 of about 5,054 (183)

Global DNA Methylation Detection System Using MBD-Fused Luciferase Based on Bioluminescence Resonance Energy Transfer Assay

, 2016
DNA methylation plays an important role in the regulation of gene expression. In normal cells, transposable elements that constitute approximately 45% of the human genome are highly methylated to silence their expression.
Yuji Baba (2534092), Wataru Yoshida (417955), Isao Karube (2868176)   +2 more
core   +1 more source

Means and methods for bioluminescence resonance energy transfer (bret) analysis in a biological sample

The invention relates to the field of in vitro detection methods using luminescence. Provided is a sensor molecule for detecting an analyte of interest in a sample using bioluminescence resonance energy transfer (BRET), the sensor molecule comprising a ...
Johnsson, Kai Peter, Schena, Alberto, Griss, Rudolf   +2 more
core  

Extended bioluminescence resonance energy transfer (eBRET) for monitoring prolonged protein–protein interactions in live cells

, 2006
Bioluminescence resonance energy transfer (BRET) is an increasingly popular technique for studying protein–protein interactions in live cells. It is particularly suitable for real-time monitoring of such interactions, however, the timescale over which ...
Dalrymple, Matthew B., Pfleger, Kevin D. G., Thomas, Walter G., Eidne, Karin A., Dromey, Jasmin R., Lim, Esther M. L.   +5 more
core   +1 more source

A universal bioluminescence resonance energy transfer sensor design enables high-sensitivity screening of GPCR activation dynamics

, 2018
G-protein-coupled receptors (GPCRs) represent one of the most important classes of drug targets. The discovery of new GCPR therapeutics would greatly benefit from the development of a generalizable high-throughput assay to directly monitor their ...
Frank, M., Schihada, Hannes, Lohse, M.J., Maiellaro, I., Vandenabeele, Sylvie, Schihada, H., Vandenabeele, S., Frank, Monika, Maiellaro, Isabella, Zabel, U., Zabel, Ulrike, Lohse, Martin J.   +11 more
core   +1 more source

Third-Party Bioluminescence Resonance Energy Transfer Indicates Constitutive Association of Membrane Proteins: Application to Class A G-Protein-Coupled Receptors and G-Proteins

, 2010
Many of the molecules that mediate G-protein signaling are thought to constitutively associate with each other in variably stable signaling complexes.
Lambert, Nevin A., Kuravi, Sudhakiranmayi, Barik, Arnab, Lan, Tien-Hung   +3 more
core   +1 more source

Unraveling ER dimerization dynamics in endocrine disruption based on a BRET-focused approach

Animal Cells and Systems
Endocrine-disrupting chemicals (EDCs) are exogenous compounds that interact with the estrogen receptor (ER), thereby disrupting estrogen-mediated signaling.
Soomin Yum, Haksoo Lee, Yong-Kook Kwon, Gunyoung Lee, Hye-Young Lee, HyeSook Youn, BuHyun Youn   +6 more
doaj   +1 more source

A Bioluminescence Resonance Energy Transfer-Based Approach for Determining Antibody-Receptor Occupancy In Vivo

, 2019
Elucidating receptor occupancy (RO) of monoclonal antibodies (mAbs) is a crucial step in characterizing the therapeutic efficacy of mAbs. However, the in vivo assessment of RO, particularly within peripheral tissues, is greatly limited by current ...
Parag-Sharma, Kshitij, Tang, Yu, Cao, Yanguang, Amelio, Antonio L.   +3 more
core   +1 more source

General Bioluminescence Resonance Energy Transfer Homogeneous Immunoassay for Small Molecules Based on Quantum Dots

, 2016
Here, we describe a general bioluminescence resonance energy transfer (BRET) homogeneous immunoassay based on quantum dots (QDs) as the acceptor and Renilla luciferase (Rluc) as the donor (QD-BRET) for the determination of small molecules.
Xiya Zhang (830294), Zhanhui Wang (160469), Chenglong Li (129885), Jianzhong Shen (261666), Xuezhi Yu (472142), Kai Wen (261662), Suxia Zhang (261656)   +6 more
core   +1 more source

Bioluminescence Resonance Energy Transfer: A Powerful Technique in Neuropharmacology for Discovering New Ligands of G Protein-Coupled Receptors

MasterThe Bioluminescence Resonance Energy Transfer (BRET) is a proximity-based assay used to study protein-protein interactions in living cells. The principle behind BRET involves resonance transfer of energy between bioluminescent enzymes and a ...
Morisset-Lopez, Séverine
core   +1 more source

SUBCELLULAR IMAGING OF DYNAMIC PROTEIN INTERACTIONS BY BIOLUMINESCENCE RESONANCE ENERGY TRANSFER.

, 2007
Despite the fact that numerous studies suggest the existence of receptor multiprotein complexes, visualization and monitoring of the dynamics of such protein assemblies remains a challenge.
Coulon, Vincent, Vincent Coulon, Martin Audet, Homburger, Vincent, Laurent Fagni, Fagni, Laurent, Michel Bouvier, Vincent Homburger, Perroy, Julie, Julie Perroy, Bouvier, Michel, Joël Bockaert, Bockaert, Joël, Audet, Martin   +13 more
core   +1 more source