Results 11 to 20 of about 8,062,393 (179)

Type-3 BRET, an Improved Competition-Based Bioluminescence Resonance Energy Transfer Assay [PDF]

Biophysical Journal, 2014
We show that in conventional, competition-based bioluminescence resonance energy transfer (BRET) assays of membrane protein stoichiometry, the presence of competitors can alter tagged-protein density and artifactually reduce energy transfer efficiency.
Felce, James H., Knox, Rachel G., Davis, Simon J.   +2 more
openaire   +4 more sources

Integration of Nanomaterials and Bioluminescence Resonance Energy Transfer Techniques for Sensing Biomolecules

Biosensors, 2019
Bioluminescence resonance energy transfer (BRET) techniques offer a high degree of sensitivity, reliability and ease of use for their application to sensing biomolecules.
Eugene Hwang, Jisu Song, Jin Zhang
doaj   +2 more sources

Developments in FRET- and BRET-Based Biosensors

Micromachines, 2022
Resonance energy transfer technologies have achieved great success in the field of analysis. Particularly, fluorescence resonance energy transfer (FRET) and bioluminescence resonance energy transfer (BRET) provide strategies to design tools for sensing ...
Yuexin Wu, Tianyu Jiang
doaj   +2 more sources

Bioluminescence Resonance Energy Transfer (BRET)-Mediated Protein Release from Self-Illuminating Photoresponsive Biomaterials. [PDF]

J Am Chem Soc
Phototriggered release of various cargos, including soluble protein factors and small molecules, has the potential to correct aberrant biological events by offering spatiotemporal control over local therapeutic levels. However, the poor penetration depth of light historically limits implementation to subdermal regions, necessitating alternative methods
Rapp TL, Kopyeva I, Adhikari A, DeForest CA.   +3 more
europepmc   +4 more sources

Mathematical Models for Quantitative Assessment of Bioluminescence Resonance Energy Transfer (BRET): Application to Seven Transmembrane Receptors (7TMRs) Oligomerization [PDF]

Frontiers in Endocrinology, 2012
The idea that seven transmembrane receptors (7TMRs; also designated G-protein coupled receptors (GPCRs)) might form dimers or higher order oligomeric complexes was formulated more than 20 years ago and has been intensively studied since then. In the last
Luka eDrinovec, Valentina eKubale, Jane eNøhr Larsen, Milka eVrecl   +3 more
doaj   +2 more sources

Monitoring Opioid Receptor Dimerization in Living Cells by Bioluminescence Resonance Energy Transfer (BRET)

, 2014
Bioluminescence resonance energy transfer (BRET) is a natural phenomenon that has been successfully applied for the study of protein-protein interactions, including opioid receptor oligomers. The discovery of opioid receptor homomers and heteromers has brought to the finding of new functions and new way of signaling and trafficking; therefore, opioid ...
Baiula, Monica, Monica Baiula
openaire   +4 more sources

Protocol to measure and analyze protein interactions in mammalian cells using bioluminescence resonance energy transfer [PDF]

STAR Protocols
Summary: Bioluminescence resonance energy transfer (BRET) allows to quantitate protein interactions in intact cells. Here, we present a protocol for measuring BRET due to transient interactions of oncogenic K-RasG12V in plasma membrane nanoclusters of ...
Carla Jane Duval, Candy Laura Steffen, Karolina Pavic, Daniel Kwaku Abankwa   +3 more
doaj   +2 more sources

Application of bioluminescence resonance energy transfer-based cell tracking approach in bone tissue engineering

Journal of Tissue Engineering, 2021
Bioluminescent imaging (BLI) has emerged as a popular in vivo tracking modality in bone regeneration studies stemming from its clear advantages: non-invasive, real-time, and inexpensive. We recently adopted bioluminescence resonance energy transfer (BRET)
Lufei Wang, Dong Joon Lee, Han Han, Lixing Zhao, Hiroshi Tsukamoto, Yong-IL Kim, Adele M Musicant, Kshitij Parag-Sharma, Xiangxiang Hu, Henry C Tseng, Jen-Tsan Chi, Zhengyan Wang, Antonio L Amelio, Ching-Chang Ko   +13 more
doaj   +2 more sources

Bioluminescent sentinel plants enable autonomous diagnostics of viral infections [PDF]

Nature Communications
Plants engineered with synthetic genetic programs can transform how we monitor and manage the extension of crop pests and diseases. Here, we establish a bioluminescent platform in Nicotiana benthamiana for autonomous viral sensing based on the fungal ...
Camilo Calvache, Marta Rodriguez-Rodriguez, Victor Vazquez-Vilriales, Elena Garcia-Perez, Aubin Fleiss, Mustafá Ezzeddin-Ayoub, Fabio Pasin, José-Antonio Daròs, Karen S. Sarkisyan, Diego Orzaez, Marta Vazquez-Vilar   +10 more
doaj   +2 more sources

Comparison of static and microfluidic protease assays using modified bioluminescence resonance energy transfer chemistry.

PLoS ONE, 2014
BackgroundFluorescence and bioluminescence resonance energy transfer (F/BRET) are two forms of Förster resonance energy transfer, which can be used for optical transduction of biosensors.
Nan Wu, Helen Dacres, Alisha Anderson, Stephen C Trowell, Yonggang Zhu   +4 more
doaj   +3 more sources