Results 11 to 20 of about 5,054 (183)

Image Processing for Bioluminescence Resonance Energy Transfer Measurement—BRET-Analyzer [PDF]

Frontiers in Computational Neuroscience, 2018
A growing number of tools now allow live recordings of various signaling pathways and protein-protein interaction dynamics in time and space by ratiometric measurements, such as Bioluminescence Resonance Energy Transfer (BRET) Imaging.
Yan Chastagnier, Yan Chastagnier, Yan Chastagnier, Enora Moutin, Enora Moutin, Enora Moutin, Anne-Laure Hemonnot, Anne-Laure Hemonnot, Anne-Laure Hemonnot, Julie Perroy, Julie Perroy, Julie Perroy   +11 more
doaj   +5 more sources

Bioluminescence Resonance Energy Transfer (BRET) Allows Monitoring the Barnase-Barstar Complex In Vivo [PDF]

Biophysica, 2022
Bioluminescence resonance energy transfer (BRET) seems to be a promising biophysical technique to study protein–protein interactions within living cells due to a very specific reaction of bioluminescence that essentially decreases the background of other
Victor Marchenkov, Tanya Ivashina, Natalia Marchenko, Vladimir Ksenzenko, Gennady Semisotnov   +4 more
doaj   +3 more sources

Type-3 BRET, an Improved Competition-Based Bioluminescence Resonance Energy Transfer Assay [PDF]

Biophysical Journal, 2014
We show that in conventional, competition-based bioluminescence resonance energy transfer (BRET) assays of membrane protein stoichiometry, the presence of competitors can alter tagged-protein density and artifactually reduce energy transfer efficiency. A
Knox, Rachel G., Davis, Simon J., Felce, James H.   +2 more
core   +3 more sources

Analysis of in vitro SUMOylation using bioluminescence resonance energy transfer (BRET)

Biochemical and Biophysical Research Communications, 2009
We demonstrated in vitro small ubiquitin-like modifier (SUMO)-mediated modification (SUMOylation) of RanGTPase activating protein-1 (RdnGAP1) by using bioluminescence resonance energy transfer (BRET) for studying protein interactions. Renilla luciferase (
Park, Sun-Young, Oh, Young-Hee, Jin, Zongwen, Kim, Eun-Kyung, Kim, Young-Pil, Kim, Hak-Sung   +5 more
core   +4 more sources

Integration of Nanomaterials and Bioluminescence Resonance Energy Transfer Techniques for Sensing Biomolecules

Biosensors, 2019
Bioluminescence resonance energy transfer (BRET) techniques offer a high degree of sensitivity, reliability and ease of use for their application to sensing biomolecules.
Eugene Hwang, Jisu Song, Jin Zhang
doaj   +2 more sources

Monitoring opioid receptor interaction in living cells by bioluminescence resonance energy transfer (bret)

, 2020
Bioluminescence resonance energy transfer (BRET) is a natural phenomenon that has been successfully applied for the study of protein–protein interactions, including opioid receptor oligomers.
Monica Baiula, Baiula M.
core   +3 more sources

Novel tools for use in Bioluminescence Resonance Energy Transfer (BRET) assays

, 2009
Recent advances in imaging assays based on bioluminescence resonance energy transfer (BRET) have made it possible to study protein/protein interactions in living cells under physiological conditions.
Alessandra Baragli, Hebert, Terence E., Baragli, Alessandra, Isabelle Héroux, Heroux, Isabelle, Robitaille, Melanie, Mélanie Robitaille, Terence E. Hébert   +7 more
core   +6 more sources

Monitoring opioid receptor dimerization in living cells by bioluminescence resonance energy transfer (BRET)

, 2014
Bioluminescence resonance energy transfer (BRET) is a natural phenomenon that has been successfully applied for the study of protein–protein interactions, including opioid receptor oligomers.
Baiula, Monica, Monica Baiula
core   +4 more sources

Mathematical Models for Quantitative Assessment of Bioluminescence Resonance Energy Transfer (BRET): Application to Seven Transmembrane Receptors (7TMRs) Oligomerization

Frontiers in Endocrinology, 2012
The idea that seven transmembrane receptors (7TMRs; also designated G-protein coupled receptors (GPCRs)) might form dimers or higher order oligomeric complexes was formulated more than 20 years ago and has been intensively studied since then. In the last
Luka eDrinovec, Valentina eKubale, Jane eNøhr Larsen, Milka eVrecl   +3 more
doaj   +2 more sources

Protocol to measure and analyze protein interactions in mammalian cells using bioluminescence resonance energy transfer [PDF]

STAR Protocols
Summary: Bioluminescence resonance energy transfer (BRET) allows to quantitate protein interactions in intact cells. Here, we present a protocol for measuring BRET due to transient interactions of oncogenic K-RasG12V in plasma membrane nanoclusters of ...
Carla Jane Duval, Candy Laura Steffen, Karolina Pavic, Daniel Kwaku Abankwa   +3 more
doaj   +2 more sources