Results 11 to 20 of about 6,388 (174)

Image Processing for Bioluminescence Resonance Energy Transfer Measurement—BRET-Analyzer [PDF]

Frontiers in Computational Neuroscience, 2018
A growing number of tools now allow live recordings of various signaling pathways and protein-protein interaction dynamics in time and space by ratiometric measurements, such as Bioluminescence Resonance Energy Transfer (BRET) Imaging.
Yan Chastagnier, Yan Chastagnier, Yan Chastagnier, Enora Moutin, Enora Moutin, Enora Moutin, Anne-Laure Hemonnot, Anne-Laure Hemonnot, Anne-Laure Hemonnot, Julie Perroy, Julie Perroy, Julie Perroy   +11 more
doaj   +5 more sources

Analysis of RXR/THR and RXR/PPARG2 heterodimerization by bioluminescence resonance energy transfer (BRET). [PDF]

PLoS ONE, 2013
BACKGROUND: Nuclear receptors (NR) regulate transcription of genes involved in many biological processes such as development, cell proliferation, differentiation and cell death.
Miquel Mulero, Julie Perroy, Carole Federici, Gérard Cabello, Vincent Ollendorff   +4 more
doaj   +10 more sources

Bioluminescence resonance energy transfer (BRET) imaging in plant seedlings and mammalian cells. [PDF]

Methods Mol Biol, 2011
Bioluminescence resonance energy transfer (BRET) has become a widely used technique to monitor protein-protein interactions. It involves resonance energy transfer between a bioluminescent donor and a fluorescent acceptor.
Xie Q, Soutto M, Xu X, Zhang Y, Johnson CH.   +4 more
europepmc   +5 more sources

Enhanced Molecular Tension Sensor Based on Bioluminescence Resonance Energy Transfer (BRET). [PDF]

ACS Sens, 2020
Molecular tension sensors measure piconewton forces experienced by individual proteins in the context of the cellular microenvironment. Current genetically encoded tension sensors use FRET to report on extension of a deformable peptide encoded in a cellular protein of interest.
Aird EJ, Tompkins KJ, Ramirez MP, Gordon WR.   +3 more
europepmc   +5 more sources

Genetically Encoded RNA-Based Bioluminescence Resonance Energy Transfer (BRET) Sensors. [PDF]

ACS Sens, 2023
Abstract RNA-based nanostructures and molecular devices have become popular for developing biosensors and genetic regulators. These programmable RNA nanodevices can be genetically encoded and modularly engineered to detect various cellular targets and then induce output signals, most often a fluorescence readout ...
Mi L, Yu Q, Karunanayake Mudiyanselage APKK, Wu R, Sun Z, Zheng R, Ren K, You M.   +7 more
europepmc   +4 more sources

AlphaScreen HTS and live-cell bioluminescence resonance energy transfer (BRET) assays for identification of Tau-Fyn SH3 interaction inhibitors for Alzheimer disease. [PDF]

J Biomol Screen, 2014
Alzheimer disease (AD) is the most common neurodegenerative disease, and with Americans’ increasing longevity, it is becoming an epidemic. There are currently no effective treatments for this disorder.
Cochran JN, Diggs PV, Nebane NM, Rasmussen L, White EL, Bostwick R, Maddry JA, Suto MJ, Roberson ED.   +8 more
europepmc   +2 more sources

Screening for Protein-Protein Interaction Inhibitors Using a Bioluminescence Resonance Energy Transfer (BRET)–Based Assay in Yeast

SLAS Discovery, 2017
The bioluminescence resonance energy transfer (BRET) technology is a widely used live cell-based method for monitoring protein-protein interactions as well as conformational changes within proteins or molecular complexes.
Caroline Corbel, S. Sartini, E. Levati, P. Colas, L. Maillet, Cyril Couturier, B. Montanini, S. Bach   +7 more
semanticscholar   +3 more sources

Genetically encoded fluorogenic RNA-based bioluminescence resonance energy transfer (BRET) sensors for cellular imaging and target detection

SLAS Discovery
Fluorescence- and bioluminescence-based probes are valuable tools for understanding cell functions in health and disease. Bioluminescence offers an ideal complementary readout to fluorescence due to its minimal background interference and self ...
Lan Mi, Mingxu You
doaj   +2 more sources

An enhanced molecular tension sensor based on bioluminescence resonance energy transfer (BRET) [PDF]

bioRxiv, 2019
Molecular tension sensors measure piconewton forces experienced by individual proteins in the context of the cellular microenvironment. Current genetically-encoded tension sensors use FRET to report on extension of an elastic peptide encoded in a ...
Eric J. Aird, K. Tompkins, W. Gordon
semanticscholar   +2 more sources

Real-time, continuous detection of maltose using bioluminescence resonance energy transfer (BRET) on a microfluidic system.

Biosensors and Bioelectronics, 2014
We have previously shown that a genetically encoded bioluminescent resonance energy transfer (BRET) biosensor, comprising maltose binding protein (MBP) flanked by a green fluorescent protein (GFP(2)) at the N-terminus and a variant of Renilla luciferase (RLuc2) at the C-terminus, has superior sensitivity and limits of detection for maltose, compared ...
N. Le, M. Gel, Yonggang Zhu, H. Dacres, A. Anderson, S. Trowell   +5 more
semanticscholar   +5 more sources