Results 31 to 40 of about 14,160 (163)
Ratiometric BRET Measurements of ATP with a Genetically-Encoded Luminescent Sensor
Sensors, 2019 Luciferase-based reporters provide a key measurement approach in a broad range of applications, from in vitro high-throughput screening to whole animal imaging.
Se-Hong Min +5 more
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Investigation of interactions between TLR2, MyD88 and TIRAP by bioluminescence resonance energy transfer is hampered by artefacts of protein overexpression. [PDF]
PLoS ONE, 2018 Toll like receptors (TLRs) are important pattern recognition receptors that can detect pathogen and danger associated molecular patterns to initiate an innate immune response.
Natália G Sampaio +4 more
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Microorganisms, 2021 Human cytomegalovirus (HCMV) genome replication is a complex and still not completely understood process mediated by the highly coordinated interaction of host and viral products.
Veronica Di Antonio +2 more
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Molecules, 2019 The bioluminescence resonance energy transfer (BRET) approach involves resonance energy transfer between a light-emitting enzyme and fluorescent acceptors.
Chayma El Khamlichi +5 more
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Bio-Protocol, 2019 Detecting protein-protein interactions by co-immunoprecipitation provided a major advancement in the immunology research field. In the G-protein-coupled receptors (GPCRs) research field, colocalization and co-immunoprecipitation were used to detect ...
Irene Reyes-Resina +3 more
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Regulator of G protein signaling 2 (RGS2) and RGS4 form distinct G protein-dependent complexes with protease activated-receptor 1 (PAR1) in live cells. [PDF]
PLoS ONE, 2014 Protease-activated receptor 1 (PAR1) is a G-protein coupled receptor (GPCR) that is activated by natural proteases to regulate many physiological actions.
Sungho Ghil +2 more
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STAR Protocols, 2022 Summary: The Kinetic Intra-Cellular Assay (KICA) is a recombinant cell-based technique that utilizes NanoBRET technology. KICA enables the measurement of intracellular binding kinetics.
Charles S. Lay +5 more
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Image Processing for Bioluminescence Resonance Energy Transfer Measurement—BRET-Analyzer
Frontiers in Computational Neuroscience, 2018 A growing number of tools now allow live recordings of various signaling pathways and protein-protein interaction dynamics in time and space by ratiometric measurements, such as Bioluminescence Resonance Energy Transfer (BRET) Imaging.
Yan Chastagnier +11 more
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NanoBRET in C. elegans illuminates functional receptor interactions in real time
BMC Molecular and Cell Biology, 2022 Background Protein-protein interactions form the basis of every organism and thus, investigating their dynamics, intracellular protein localization, trafficking and interactions of distinct proteins such as receptors and their ligand-binding are of ...
Victoria Elisabeth Groß +4 more
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Luciferase-induced photoreductive uncaging of small-molecule effectors
Nature Communications, 2018 Bioluminescence resonance energy transfer (BRET) has been mostly employed in imaging applications. Here the authors use BRET to activate a ruthenium-based photocatalyst and perform a bioorthogonal chemical reaction, which can be used to uncage small ...
Eric Lindberg +3 more
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