Results 11 to 20 of about 131,173 (232)

Bret/ BRAT

Critical Quarterly
Critical Quarterly, EarlyView.
Nicholas Smart
openaire   +2 more sources

Advances in BRET probes for intracellular target engagement studies. [PDF]

Nat Chem Biol
Assessing drug–target engagement in living cells is essential for verifying the activity of pharmacological agents. Traditional binding assays often overlook key factors such as permeability, intracellular distribution and complex formation that ...
Capener JL, Schwalm MP, Vasta JD, Michaud A, Teske KA, Marsiglia WM, Huber KVM, Dar AC, Knapp S, Axtman AD, Robers MB.   +10 more
europepmc   +2 more sources

Rapid and Reliable Quantification of Prime Editing Targeting Within the Porcine ABCA4 Gene Using a BRET-Based Sensor

Nucleic Acid Therapeutics, 2023
Stargardt disease (STGD) leads to blindness in children and young adults. So far, no curative therapy is available and gene augmentation therapies have not yet advanced to the clinics, in part, due to the limited packaging capacity of adeno-associated ...
Tobias Wimmer, Hannah Sawinski, A. Urban, J. Motlík, K. Stieger   +4 more
semanticscholar   +1 more source

Genetically encoded BRET-activated photodynamic therapy for the treatment of deep-seated tumors

Light: Science & Applications, 2022
Photodynamic therapy (PDT) is one of the most appealing photonic modalities for cancer treatment based on anticancer activity of light-induced photosensitizer-mediated reactive oxygen species (ROS), but a limited depth of light penetration into tissues ...
E. Shramova, S. Chumakov, Victoria O. Shipunova, A. Ryabova, G. Telegin, A. Kabashin, S. Deyev, G. Proshkina   +7 more
semanticscholar   +1 more source

Quantitative assessment of constitutive G protein-coupled receptor activity with BRET-based G protein biosensors

bioRxiv, 2021
Heterotrimeric G proteins constitute the primary transducers of G protein-coupled receptor (GPCR) signaling. Besides mediating ligand-induced GPCR activation, G proteins transduce basal levels of activity in various physiological and pathophysiological ...
Hannes Schihada, Rawan Shekhani, G. Schulte   +2 more
semanticscholar   +1 more source

All-in-one theranostic nano-platform based on polymer nanoparticles for BRET/FRET-initiated bioluminescence imaging and synergistically anti-inflammatory therapy for ulcerative colitis

Journal of Nanobiotechnology, 2022
Background Ulcerative colitis (UC), a subtype of inflammatory bowel disease (IBD), has evolved into a global burden given its high incidence. There is a clinical need to create better diagnostic and therapeutic approaches to UC.
X. Yan, Chunhua Yang, Mei Yang, Yana Ma, Yuanyuan Zhang, Yujie Zhang, Cui Liu, Qiuran Xu, K. Tu, Mingzhen Zhang   +9 more
semanticscholar   +1 more source

A genetically encoded BRET-based SARS-CoV-2 Mpro protease activity sensor

bioRxiv, 2022
The main protease, M pro , is critical for SARS-CoV-2 replication and an appealing target for designing anti-SARS-CoV-2 agents. Therefore, there is a demand for the development of improved sensors to monitor its activity.
Anupriya M. Geethakumari, Wesam S. Ahmed, Saad Rasool, Asmat Fatima, Nasir Uddin, Mustapha Aouida, K. Biswas   +6 more
semanticscholar   +1 more source

BRET-Based Biosensors to Measure Agonist Efficacies in Histamine H1 Receptor-Mediated G Protein Activation, Signaling and Interactions with GRKs and β-Arrestins

International Journal of Molecular Sciences, 2022
The histamine H1 receptor (H1R) is a G protein-coupled receptor (GPCR) and plays a key role in allergic reactions upon activation by histamine which is locally released from mast cells and basophils.
E. W. E. Verweij, R. Bosma, Meichun Gao, Jelle van den Bor, Betty Al Araaj, Sabrina M. de Munnik, Xiaoyuan Ma, R. Leurs, H. Vischer   +8 more
semanticscholar   +1 more source

Genetically Encoded RNA-based Bioluminescence Resonance Energy Transfer (BRET) Sensors

bioRxiv, 2022
RNA-based nanostructures and molecular devices have become popular for developing biosensors and genetic regulators. These programmable RNA nanodevices can be genetically encoded and modularly engineered to detect various cellular targets and then induce
Lan Mi, Qikun Yu, A. K. Mudiyanselage, Rigumula Wu, Zhining Sun, R. Zheng, Kewei Ren, Mingxu You   +7 more
semanticscholar   +1 more source

Insertion of Nanoluc into the Extracellular Loops as a Complementary Method To Establish BRET-Based Binding Assays for GPCRs

ACS Pharmacology & Translational Science, 2022
Luminescence-based techniques play an increasingly important role in all areas of biochemical research, including investigations on G protein-coupled receptors (GPCRs).
Lukas Grätz, Christoph Müller, Andrea Pegoli, L. Schindler, G. Bernhardt, Timo Littmann   +5 more
semanticscholar   +1 more source