Results 31 to 40 of about 467,470 (351)

Overexpression of biotin synthase and biotin ligase is required for efficient generation of sulfur-35 labeled biotin in E. coli

BMC Biotechnology, 2010
Background Biotin is an essential enzyme cofactor that acts as a CO2 carrier in carboxylation and decarboxylation reactions. The E. coli genome encodes a biosynthetic pathway that produces biotin from pimeloyl-CoA in four enzymatic steps. The final step,
Prigge Sean T, Spalding Maroya D, Delli-Bovi Teegan A   +2 more
doaj   +1 more source

Advanced Resistance Studies Identify Two Discrete Mechanisms in Staphylococcus aureus to Overcome Antibacterial Compounds that Target Biotin Protein Ligase

Antibiotics, 2020
Biotin protein ligase (BPL) inhibitors are a novel class of antibacterial that target clinically important methicillin-resistant Staphylococcus aureus (S. aureus). In S. aureus, BPL is a bifunctional protein responsible for enzymatic biotinylation of two
Andrew J. Hayes, Jiulia Satiaputra, Louise M. Sternicki, Ashleigh S. Paparella, Zikai Feng, Kwang J. Lee, Beatriz Blanco-Rodriguez, William Tieu, Bart A. Eijkelkamp, Keith E. Shearwin, Tara L. Pukala, Andrew D. Abell, Grant W. Booker, Steven W. Polyak   +13 more
doaj   +1 more source

Construction of Orthogonal Modular Proteinaceous Nanovaccine Delivery Vectors Based on mSA-Biotin Binding

Nanomaterials, 2022
Proteinaceous nanovaccine delivery systems have significantly promoted the development of various high-efficiency vaccines. However, the widely used method of coupling the expression of scaffolds and antigens may result in their structural interference ...
Yixin Shi, Chao Pan, Kangfeng Wang, Yan Liu, Yange Sun, Yan Guo, Peng Sun, Jun Wu, Ying Lu, Li Zhu, Hengliang Wang   +10 more
doaj   +1 more source

Protein-mediated dethreading of a biotin-functionalised pseudorotaxane [PDF]

, 2014
In this article, we describe the synthesis of new biotin-functionalised naphthalene derivatives 3 and 4 and their complexation behaviour with avidin and neutravidin using a range of analytical techniques.
Caldwell, S.T., Cooke, G., Cooper, A., Fitzpatrick, B., Khaled, B., Maclean, C., Nutley, M., Rabani, G., Riehle, M., Rotello, V.M., Smith, B.O., Woisel, P.   +11 more
core   +3 more sources

Biotin dependency due to a defect in biotin transport [PDF]

Journal of Clinical Investigation, 2002
We describe a 3-year-old boy with biotin dependency not caused by biotinidase, holocarboxylase synthetase, or nutritional biotin deficiency. We sought to define the mechanism of his biotin dependency. The child became acutely encephalopathic at age 18 months. Urinary organic acids indicated deficiency of several biotin-dependent carboxylases.
Rebecca, Mardach, Janos, Zempleni, Barry, Wolf, Martin J, Cannon, Michael L, Jennings, Sally, Cress, Jane, Boylan, Susan, Roth, Stephen, Cederbaum, Donald M, Mock   +9 more
openaire   +2 more sources

A Stable Biotin-Streptavidin Surface Enables Multiplex, Label-Free Protein Detection by Aptamer and Aptamer-Protein Arrays Using Arrayed Imaging Reflectometry

Sensors, 2020
While label-free multiplex sensor technology enables “mixing and matching” of different capture molecules in principle, in practice this has been rarely (if ever) demonstrated. To fill this gap, we developed protocols for the preparation of mixed aptamer-
Alanna M. Klose, Benjamin L. Miller
doaj   +1 more source

Sensitive protein detection using an optical fibre long period grating sensor anchored with silica core gold shell nanoparticles [PDF]

, 2014
Copyright ©2014 Society of Photo-Optical Instrumentation Engineers. This paper was published in the Proceedings of SPIE and is made available with permission of SPIE. One print or electronic copy may be made for personal use only.
Clark, M., Hernandez, F. U., James, Stephen W., Korposh, Sergiy, Marques, L., Morgan, S. P., Tatam, Ralph P.   +6 more
core   +1 more source

Scientific Opinion on Dietary Reference Values for biotin [PDF]

EFSA Journal, 2014
Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies (NDA) derived Dietary Reference Values (DRVs) for biotin.
EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA)
doaj   +1 more source

Media Supplementation with Mannitol and Biotin Enhances Squalene Production of Thraustochytrium ATCC 26185 through Increased Glucose Uptake and Antioxidative Mechanisms

Molecules, 2022
Media supplementation with exogenous chemicals is known to stimulate the accumulation of important lipids produced by microalgae and thraustochytrids.
M. Kashif Ali, Biswarup Sen, Yaodong He, Mohan Bai, Guangyi Wang   +4 more
doaj   +1 more source

Metabolism of Biotin and Analogues of Biotin by Microorganisms [PDF]

Journal of Bacteriology, 1966
Birnbaum, Jerome (University of Cincinnati, Cinncinati, Ohio), and Herman C. Lichstein . Metabolism of biotin and analogues of biotin by microorganisms. II. Further studies on the conversion of d -biotin to biotin vitamers by Lactobacillus plantarum .
J, Birnbaum, H C, Lichstein
openaire   +2 more sources