Results 31 to 40 of about 338,983 (344)

Advanced Resistance Studies Identify Two Discrete Mechanisms in Staphylococcus aureus to Overcome Antibacterial Compounds that Target Biotin Protein Ligase

Antibiotics, 2020
Biotin protein ligase (BPL) inhibitors are a novel class of antibacterial that target clinically important methicillin-resistant Staphylococcus aureus (S. aureus). In S. aureus, BPL is a bifunctional protein responsible for enzymatic biotinylation of two
Andrew J. Hayes, Jiulia Satiaputra, Louise M. Sternicki, Ashleigh S. Paparella, Zikai Feng, Kwang J. Lee, Beatriz Blanco-Rodriguez, William Tieu, Bart A. Eijkelkamp, Keith E. Shearwin, Tara L. Pukala, Andrew D. Abell, Grant W. Booker, Steven W. Polyak   +13 more
doaj   +1 more source

Construction of Orthogonal Modular Proteinaceous Nanovaccine Delivery Vectors Based on mSA-Biotin Binding

Nanomaterials, 2022
Proteinaceous nanovaccine delivery systems have significantly promoted the development of various high-efficiency vaccines. However, the widely used method of coupling the expression of scaffolds and antigens may result in their structural interference ...
Yixin Shi, Chao Pan, Kangfeng Wang, Yan Liu, Yange Sun, Yan Guo, Peng Sun, Jun Wu, Ying Lu, Li Zhu, Hengliang Wang   +10 more
doaj   +1 more source

A Stable Biotin-Streptavidin Surface Enables Multiplex, Label-Free Protein Detection by Aptamer and Aptamer-Protein Arrays Using Arrayed Imaging Reflectometry

Sensors, 2020
While label-free multiplex sensor technology enables “mixing and matching” of different capture molecules in principle, in practice this has been rarely (if ever) demonstrated. To fill this gap, we developed protocols for the preparation of mixed aptamer-
Alanna M. Klose, Benjamin L. Miller
doaj   +1 more source

Overexpression of biotin synthase and biotin ligase is required for efficient generation of sulfur-35 labeled biotin in E. coli

BMC Biotechnology, 2010
Background Biotin is an essential enzyme cofactor that acts as a CO2 carrier in carboxylation and decarboxylation reactions. The E. coli genome encodes a biosynthetic pathway that produces biotin from pimeloyl-CoA in four enzymatic steps. The final step,
Prigge Sean T, Spalding Maroya D, Delli-Bovi Teegan A   +2 more
doaj   +1 more source

Scientific Opinion on Dietary Reference Values for biotin [PDF]

EFSA Journal, 2014
Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies (NDA) derived Dietary Reference Values (DRVs) for biotin.
EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA)
doaj   +1 more source

Media Supplementation with Mannitol and Biotin Enhances Squalene Production of Thraustochytrium ATCC 26185 through Increased Glucose Uptake and Antioxidative Mechanisms

Molecules, 2022
Media supplementation with exogenous chemicals is known to stimulate the accumulation of important lipids produced by microalgae and thraustochytrids.
M. Kashif Ali, Biswarup Sen, Yaodong He, Mohan Bai, Guangyi Wang   +4 more
doaj   +1 more source

Biotin Homeostasis and Human Disorders: Recent Findings and Perspectives

International Journal of Molecular Sciences
Biotin (vitamin B7, or vitamin H) is a water-soluble B-vitamin that functions as a cofactor for carboxylases, i.e., enzymes involved in the cellular metabolism of fatty acids and amino acids and in gluconeogenesis; moreover, as reported, biotin may be ...
Chrysoula-Evangelia Karachaliou, E. Livaniou   +1 more
semanticscholar   +1 more source

Chemistry of Biotin–Streptavidin and the Growing Concern of an Emerging Biotin Interference in Clinical Immunoassays

ACS Omega, 2019
Overconsumption of biotin (5–100 mg daily) as a supplement by the general population poses a significant problem for clinical immunoassays (IAs) based on biotin–streptavidin (SA) interactions. This affinity pair has been exploited in immunoassays because
J. Luong, S. K. Vashist
semanticscholar   +1 more source

Synthesis and Antitumor Evaluation of Biotin-SN38-Valproic Acid Conjugates

Molecules, 2023
Despite the strong anticancer activity of SN38 (7-ethyl-10-hydroxy-camptothecin), the severe side effects and loss of anticancer activity caused by the lack of selectivity to cancer cells and hydrolysis of ring E prevent its clinical application.
Yi Dai, Yang Zhang, Tianxiang Ye, Yue Chen   +3 more
doaj   +1 more source

Transungual Delivery, Anti-Inflammatory Activity, and In Vivo Assessment of a Cyclodextrin Polypseudorotaxanes Nail Lacquer

Pharmaceutics, 2020
A new cyclodextrin polypseudorotaxanes nail lacquer (Regenail®) containing biotin, methyl sulphonyl methane (MSM), and dimethylsilanediol salicylate was developed and evaluated in vitro and in vivo.
Francisco Fernández-Campos, Francesc Navarro, Adrian Corrales, Jordi Picas, Eloy Pena, Jordi González, Francisco J. Otero-Espinar   +6 more
doaj   +1 more source