Results 71 to 80 of about 452,561 (294)

Selective targeting of proteins by hybrid polyoxometalates: Interaction between a bis-biotinylated hybrid conjugate and avidin [PDF]

, 2018
The Keggin-type polyoxometalate [\u3b3-SiW10O36]8 12 was covalently modified to obtain a bis-biotinylated conjugate able to bind avidin. Spectroscopic studies such as UV-vis, fluorimetry, circular dichroism, coupled to surface plasmon resonance technique
Alessandra Saccani, Alessandro Cazzolaro, Disma Mastrogiacomo, Elisa Lubian, Fabrizio Mancin, Gloria Modugno, Livia Giotta, Ludovico Valli, Marcella Bonchio, Mauro Carraro, Silke Krol, Silke Krol, Valeria A. Zamolo   +12 more
core   +2 more sources

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Composição química dos cascos de eqüinos das raças Pantaneira e Mangalarga Marchador Chemical composition of black versus non-pigmented hooves from Pantaneira and Mangalarga Marchador horses

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2005
Pesquisaram-se eventuais diferenças na composição da matéria seca (MS), proteína bruta, extrato etéreo, cinzas, cálcio, fósforo, cobre, zinco, perfil de aminoácidos e biotina entre cascos pretos e claros, de eqüinos das raças Pantaneira e Mangalarga ...
G.A. Faria, A.S.C. Rezende, I.B.M. Sampaio, A.M.Q. Lana, R.S. Moura, J.S. Madureira, M.C. Resende   +6 more
doaj   +1 more source

DNA‐Templated 2D Heterostructures as Phototriggered Dynamic Nanohybrids: From Releasing Molecular Loads to Controlling Enzyme Biocatalytic Function

Advanced Functional Materials, EarlyView.
DNA strands are employed both as dynamic linkers and nanoscale templates for the integration of Ag2S nanoparticles on MoS2, which in turn imparted photothermal responsiveness; this feature permits the selective cargo (fluorophore, quantum dots or an enzyme) release from the MoS2 surface in response to local heat induced by light irradiation.
Kai Chen, Haosen Miao, Weiying Hong, Matteo Palma   +3 more
wiley   +1 more source

Probing the Catalytic Roles of Arg548 and Gln552 in the Carboxyl Transferase Domain of the \u3cem\u3eRhizobium etli\u3c/em\u3e Pyruvate Carboxylase by Site-directed Mutagenesis [PDF]

, 2010
The roles of Arg548 and Gln552 residues in the active site of the carboxyl transferase domain of Rhizobium etli pyruvate carboxylase were investigated using site-directed mutagenesis.
Adina-Zada, Abdussalam, Attwood, Paul V., Byrne, Lindsay, Cleland, W. Wallace, Duangpan, Saowapa, Jitrapakdee, Sarawut, Maurice, Martin St., Wallace, John C., Zeczycki, Tonya N.   +8 more
core   +1 more source

Immunostaining of skeletal tissues [PDF]

, 2011
Peer ...
De Bari, Cosimo, Kurth, Tobias B
core   +1 more source

Biotin [PDF]

Advances in Nutrition, 2012
Janos, Zempleni, Toshinobu, Kuroishi
openaire   +3 more sources

Gut microbiome and metabolome to discover pathogenic bacteria and probiotics in ankylosing spondylitis

Frontiers in Immunology
ObjectivePrevious research has partially revealed distinct gut microbiota in ankylosing spondylitis (AS). In this study, we performed non-targeted fecal metabolomics in AS in order to discover the microbiome–metabolome interface in AS.
Yupeng Lai, Yupeng Lai, Wenli Tang, Xiao Luo, Huihui Zheng, Yanpeng Zhang, Meiying Wang, Guangchuang Yu, Min Yang   +8 more
doaj   +1 more source

Impact of Surface Functionalization on NV Quantum Properties: Implications for Biosensing with Fluorescent Nanodiamonds

Advanced Functional Materials, EarlyView.
Fluorescent nanodiamonds (fNDs) have emerged as an invaluable quantum sensing platform for biological and biochemical systems. This paper investigates the influence of common surface functionalization strategies for bioconjugation on the quantum properties of nitrogen vacancy (NV) centers in nanodiamonds.
Anja Sadžak, Iván Pérez, Mateja Guimarães, Lukas Spantzel, Fedor Jelezko, Michael Börsch, Anke Krueger   +6 more
wiley   +1 more source

VIVID: A qPCR‐Based Platform for Sensitive and Quantitative In Vivo Tracking of Extracellular Vesicles

Advanced Functional Materials, EarlyView.
This study introduces VIVID (Vesicle In Vivo Identification using DNA), a qPCR‐based platform that tracks PCR‐amplifiable DNA tags loaded in the EVs for accurate and quantifiable EV biodistribution in vivo. ABSTRACT Extracellular vesicles (EVs) represent promising carriers for nucleic acid therapeutics, offering advantages over synthetic nanoparticles ...
Oscar Boyadjian, Talin Ebrahimian, Jonathan Miranda O'Connor, Janusz Rak, Stephanie Lehoux, Maryam Tabrizian   +5 more
wiley   +1 more source