Efficient Cryopreservation of Porcine Blastocysts produced by In vitro Fertilization
Cryopreservation has been applied successfully in many mammalian species. Nevertheless, pig embryos, because of their greater susceptibility to cryoinjuries, have shown a reduced developmental competence.
Sung-Hun Min+2 more
doaj +1 more source
Purpose This study compared Gavi®, an automated system for the equilibration and dehydration steps of vitrification, and a manual vitrification procedure in terms of effects on clinical outcomes. Methods The authors retrospectively compared survival rate,
Atsuko Miwa+7 more
doaj +1 more source
Embryo culture in presence of oviductal fluid induces DNA methylation changes in bovine blastocysts [PDF]
During the transit through the oviduct, the early embryo initiates an extensive DNA methylation reprogramming of its genome. Given that these epigenetic modifications are susceptible to environmental factors, components present in the oviductal milieu ...
Barrera, Antonio Daniel+7 more
core +1 more source
Human embryos from overweight and obese women display phenotypic and metabolic abnormalities [PDF]
STUDY QUESTION Is the developmental timing and metabolic regulation disrupted in embryos from overweight or obese women? SUMMARY ANSWER Oocytes from overweight or obese women are smaller than those from women of healthy weight, yet post-fertilization ...
Leary, C.+5 more
core +1 more source
Aneuploidy in the Human Blastocyst
Studies of human cleavage stage embryos, 3 days after fertilization of the oocyte, have revealed remarkably high levels of chromosome abnormality. In addition to meiotic errors derived from the gametes, principally the oocyte, mitotic errors occurring after fertilization are also common, leading to widespread chromosomal mosaicism.
Fragouli, E, Wells, D
openaire +3 more sources
TFAP2C regulates transcription in human naive pluripotency by opening enhancers. [PDF]
Naive and primed pluripotent human embryonic stem cells bear transcriptional similarity to pre- and post-implantation epiblast and thus constitute a developmental model for understanding the pluripotent stages in human embryo development. To identify new
Chen, Di+10 more
core +2 more sources
Cryopreservation of equine oocytes: looking into the crystal ball [PDF]
In vitro embryo production has evolved rapidly in the horse over the past decade, but blastocyst rates from vitrified equine oocytes remain quite poor and further research is needed to warrant application.
Angel Velez, Daniel+4 more
core +2 more sources
Regulated mitochondrial DNA replication during oocyte maturation is essential for successful porcine embryonic development. [PDF]
Cellular ATP is mainly generated through mitochondrial oxidative phosphorylation, which is dependent on mitochondrial DNA (mtDNA). We have previously demonstrated the importance of oocyte mtDNA for porcine and human fertilization.
Alexander, Jan+26 more
core +7 more sources
Development and validation of deep learning based embryo selection across multiple days of transfer [PDF]
This work describes the development and validation of a fully automated deep learning model, iDAScore v2.0, for the evaluation of embryos incubated for 2, 3, and 5 or more days. The model is trained and evaluated on an extensive and diverse dataset including 181,428 embryos from 22 IVF clinics across the world.
arxiv
Regulation of blastocyst formation
Preimplantation or pre-attachment development encompasses the "free"-living period of mammalian embryogenesis, which directs development of the zygote through to the blastocyst stage. Blastocyst formation is essential for implantation, establishment of pregnancy and is a principal determinant of embryo quality prior to embryo transfer.
Watson, A J, Barcroft, L C
openaire +5 more sources