Results 41 to 50 of about 89,965 (323)

Efficient Cryopreservation of Porcine Blastocysts produced by In vitro Fertilization

open access: yesJournal of Animal Reproduction and Biotechnology, 2016
Cryopreservation has been applied successfully in many mammalian species. Nevertheless, pig embryos, because of their greater susceptibility to cryoinjuries, have shown a reduced developmental competence.
Sung-Hun Min   +2 more
doaj   +1 more source

Equivalent clinical outcome after vitrified‐thawed blastocyst transfer using semi‐automated embryo vitrification system compared with manual vitrification method

open access: yesReproductive Medicine and Biology, 2020
Purpose This study compared Gavi®, an automated system for the equilibration and dehydration steps of vitrification, and a manual vitrification procedure in terms of effects on clinical outcomes. Methods The authors retrospectively compared survival rate,
Atsuko Miwa   +7 more
doaj   +1 more source

Embryo culture in presence of oviductal fluid induces DNA methylation changes in bovine blastocysts [PDF]

open access: yes, 2017
During the transit through the oviduct, the early embryo initiates an extensive DNA methylation reprogramming of its genome. Given that these epigenetic modifications are susceptible to environmental factors, components present in the oviductal milieu ...
Barrera, Antonio Daniel   +7 more
core   +1 more source

Human embryos from overweight and obese women display phenotypic and metabolic abnormalities [PDF]

open access: yes, 2014
STUDY QUESTION Is the developmental timing and metabolic regulation disrupted in embryos from overweight or obese women? SUMMARY ANSWER Oocytes from overweight or obese women are smaller than those from women of healthy weight, yet post-fertilization ...
Leary, C.   +5 more
core   +1 more source

Aneuploidy in the Human Blastocyst

open access: yesCytogenetic and Genome Research, 2011
Studies of human cleavage stage embryos, 3 days after fertilization of the oocyte, have revealed remarkably high levels of chromosome abnormality. In addition to meiotic errors derived from the gametes, principally the oocyte, mitotic errors occurring after fertilization are also common, leading to widespread chromosomal mosaicism.
Fragouli, E, Wells, D
openaire   +3 more sources

TFAP2C regulates transcription in human naive pluripotency by opening enhancers. [PDF]

open access: yes, 2018
Naive and primed pluripotent human embryonic stem cells bear transcriptional similarity to pre- and post-implantation epiblast and thus constitute a developmental model for understanding the pluripotent stages in human embryo development. To identify new
Chen, Di   +10 more
core   +2 more sources

Cryopreservation of equine oocytes: looking into the crystal ball [PDF]

open access: yes, 2020
In vitro embryo production has evolved rapidly in the horse over the past decade, but blastocyst rates from vitrified equine oocytes remain quite poor and further research is needed to warrant application.
Angel Velez, Daniel   +4 more
core   +2 more sources

Regulated mitochondrial DNA replication during oocyte maturation is essential for successful porcine embryonic development. [PDF]

open access: yes, 2006
Cellular ATP is mainly generated through mitochondrial oxidative phosphorylation, which is dependent on mitochondrial DNA (mtDNA). We have previously demonstrated the importance of oocyte mtDNA for porcine and human fertilization.
Alexander, Jan   +26 more
core   +7 more sources

Development and validation of deep learning based embryo selection across multiple days of transfer [PDF]

open access: yesarXiv, 2022
This work describes the development and validation of a fully automated deep learning model, iDAScore v2.0, for the evaluation of embryos incubated for 2, 3, and 5 or more days. The model is trained and evaluated on an extensive and diverse dataset including 181,428 embryos from 22 IVF clinics across the world.
arxiv  

Regulation of blastocyst formation

open access: yesFrontiers in Bioscience, 2001
Preimplantation or pre-attachment development encompasses the "free"-living period of mammalian embryogenesis, which directs development of the zygote through to the blastocyst stage. Blastocyst formation is essential for implantation, establishment of pregnancy and is a principal determinant of embryo quality prior to embryo transfer.
Watson, A J, Barcroft, L C
openaire   +5 more sources

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