Results 241 to 250 of about 1,050,605 (265)

Blocking of Non-Specific Sorption in Elisa on Formylated Polystyrene Beads

Journal of Immunoassay, 1999
The effect of pH of the blocking solution on the non-specific sorption of peroxidase conjugates to the 0.63-cm formylated polystyrene beads was studied. Anti-G-HRP and BSA-HRP sorption was shown to dramatically depend on pH of blocking protein solutions and was minimum at pH=pI. Sorption of G-HRP weakly depended on pH of the blocking buffer.
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A Monoclonal Antibody Blocking Elisa for the Detection of IBV Antibodies in Fowl

1998
A murine monoclonal antibody (mAb) reacting with the spike protein of seven field and laboratory strains of Infectious Bronchitis Virus (IBV) was characterised. Neutralisation tests performed in chicken tracheal organ culture showed that the mAb is directed against a conserved neutralising epitope.
V, Moving, G, Czifra, L, Renström
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Blocking ELISA Using Recombinant NcSRS2 Protein for Diagnosing Bovine Neosporosis

Current Microbiology, 2014
Neospora caninum is the etiologic agent of neosporosis, which leads to economic impacts on cattle industry. The reference method for serodiagnosis of neosporosis is the indirect fluorescent antibody test (IFAT). However, IFAT is laborious, expensive, and is not practicable in high throughput screening.
Francine A, Sinnott, Leonardo G, Monte, Thais F, Collares, Bruno M, De Matos, Diene B, Pacheco, Sibele, Borsuk, Renato, Andreotti, Cláudia P, Hartleben   +7 more
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ELISA test for the detection of an immunological blocking factor in human pregnancy serum

Journal of Reproductive Immunology, 1989
We have previously shown that progesterone-treated lymphocytes of healthy pregnant women can produce a 34 kDa protein, progesterone-induced blocking factor (PIBF) capable of blocking lymphocyte function in vitro. Lymphocytes of women with idiopathic threatened pre-term delivery failed to produce this factor.
J, Szekeres-Bartho, P, Varga, B, Pejtsik
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Optimization and validation of a blocking ELISA for quantitation of anti-rabies immunoglobulins in multispecies sera

Applied Microbiology and Biotechnology, 2020
We developed a fast, rabies virus-free, in vitro method, based on a blocking ELISA (bELISA), to detect and accurately quantify anti-rabies glycoprotein antibodies in serum of several animal species. In this method, purified rabies virus-like particles (VLPs) are used as antigen to coat the plates, while the presence of specific rabies immunoglobulins ...
Diego Fontana, María Celeste Rodriguez, Ernesto Garay, Susana Russo, Claudio Prieto   +4 more
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Validation of a screening liquid phase blocking ELISA for swine vesicular disease

Journal of Virological Methods, 1995
A direct liquid-phase blocking ELISA (LPBE) was developed for serological examination of swine vesicular disease (SVD). The sensitivity and specificity of the test were assessed on 272 and 365 sera collected on two farms where an outbreak had occurred. The specificity of the direct LPBE was higher than the specificity of the indirect LPBE. The European
Dekker, A., Moonen, P.L.J.M., Terpstra, C.   +2 more
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A blocking ELISA using a monoclonal antibody for the serological detection of Mycoplasma hyopneumoniae

Research in Veterinary Science, 1994
A blocking ELISA was developed by using a monoclonal antibody (4082-05-344-18) which specifically detected an epitope on the Mycoplasma hyopneumoniae 40 kDa membrane protein without cross-reacting with M flocculare or M hyorhinis. The results obtained with sera from specific pathogen-free pigs inoculated with M flocculare or M hyorhinis confirmed the ...
M F, Le Potier, P, Abiven, M, Kobisch, D, Crevat, P, Desmettre   +4 more
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Foot-and-mouth disease: detection of antibodies in cattle sera by blocking ELISA

Veterinary Microbiology, 1992
A blocking ELISA was developed for the detection of antibodies to foot-and-mouth disease virus SAT1, SAT2 and SAT3 and for the quantification of antibodies on a single dilution of serum. The avidin-biotin system was used. The test was compared with the liquid-phase ELISA executed at the World Reference Laboratory for foot-and-mouth disease.
K J, Sorensen, R L, Madekurozwa, P, Dawe
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Development and application of blocking ELISA to detect antibodies against Salmonella Enteritidis

Veterinary Microbiology
Salmonella is a foodborne zoonotic pathogen that poses a significant threat to public health. Contaminated poultry meat and eggs are major sources of Salmonella infection in humans. In recent years, Salmonella Enteritidis (S. Enteritidis) has become prevalent in large-scale poultry farms worldwide, highlighting the urgent need for a rapid detection ...
Xinyuan, Miao, Yueyue, Shang, Zimin, Huang, Jun, Liu, Xilong, Kang, Xinan, Jiao, Chuang, Meng, Zhiming, Pan   +7 more
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A sensitive epitope-blocking ELISA for the detection of Chikungunya virus-specific antibodies in patients

Journal of Virological Methods, 2015
Chikungunya fever (CHIKF) has re-emerged as an arboviral disease that mimics clinical symptoms of other diseases such as dengue, malaria, as well as other alphavirus-related illnesses leading to problems with definitive diagnosis of the infection. Herein we describe the development and evaluation of a sensitive epitope-blocking ELISA (EB-ELISA) capable
Goh, L.Y.H., Kam, Y.W., Metz, S.W.H., Hobson-Peters, J., Prow, N.A., McCarthy, S., Smith, D.W., Pijlman, G.P., Ng, L.F.P., Hall, R.A.   +9 more
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