Results 231 to 240 of about 1,050,605 (265)

Comparison of Blocking Agents for an Elisa for Lps

Journal of Immunoassay, 2000
ELISA is a sensitive, specific, reproducible and fast method for detection of antigen-antibody reactions. In case of non-protein antigens as LPS, problems exist, such as poor proportion of coating to microplates, non-specific binding of antibodies to the plastic wells.
Z, Péterfi, B, Kocsis
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Aujeszky's disease: Blocking ELISA for the detection of serum antibodies

Journal of Virological Methods, 1986
A blocking ELISA for the detection of serum antibodies to Aujeszky's disease virus has been developed. The test was designed in particular with a view to examining large numbers of blood samples. It has been found to be sensitive, specific and precise.
K J, Sørensen, J C, Lei
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Effective Blocking and Stabilizing Methods Using Synthetic Polymer on ELISA

2023
In ELISA, blocking reagents and stabilizers are important to improve the sensitivity and/or quantitative nature of the measurement system. Usually, biological substances such as bovine serum albumin and casein are used, but they still have problems such as lot-to-lot differences and biohazard.
Tomozumi, Noda, Masaru, Matsuda, Hirotaka, Suzuki, Yusuke, Okawa, Motohiro, Mitani   +4 more
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A blocking agent and a blocking step are not needed in ELISA, immunostaining dot-blots and Western blots

Journal of Immunological Methods, 1989
The effect of blocking and non-blocking was compared in ELISA, immunostaining dot-blots and western blots using phosphate-buffered saline with and without the addition of Tween 20 for washes and dilutions. The results indicated that when Tween 20 was included in phosphate-buffered saline, a blocking agent and a blocking step were not required in ...
K, Mohammad, A, Esen
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A monoclonal blocking-ELISA for detection of orthopoxvirus antibodies in feline sera

Veterinary Microbiology, 1996
A double sandwich blocking-ELISA using a genus-specific neutralizing monoclonal antibody (MAb) against the vaccinia virus 32 kD adsorption protein (D8L open reading frame: ORF) was developed to detect orthopoxvirus (OPV) antibodies in sera. A collection of 2173 feline serum samples was examined in an epidemiological study.
C P, Czerny, K, Wagner, K, Gessler, A, Mayr, O R, Kaaden   +4 more
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Development of a blocking ELISA for Caprine parainfluenza virus type 3

Journal of Virological Methods, 2017
Caprine parainfluenza virus type 3 (CPIV3) is a novel pathogen mainly causing respiratory diseases in goats. At present, there are no high throughput and rapid testing methods available for epidemiological investigation. In this study, we designed a modified method for selection of hybridomas that secrete monoclonal antibodies (mAb) specific for CPIV3.
Li, Mao, Wenliang, Li, Tianci, Zhou, Leilei, Yang, Fei, Hao, Jizong, Li, Wenwen, Zhang, Xuenong, Luo, Jieyuan, Jiang   +8 more
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Development of a Monoclonal Blocking ELISA for the Detection of Antibody to Mycoplasma bovis in Dairy Cattle and Comparison to Detection by PCR

Veterinary Immunology and Immunopathology, 2005
A monoclonal antibody blocking enzyme-linked immunosorbent assay (B-ELISA) was developed to detect antibodies to Mycoplasma bovis in cattle sera. The assay was highly specific and sensitive and there was no cross-reaction detected. This method revealed a
Robert G Hirst
exaly   +2 more sources

Development and evaluation of a blocking ELISA for serological diagnosis of equine infectious anemia

Applied Microbiology and Biotechnology, 2023
Equine infectious anemia (EIA) is an important viral disease characterized by persistent infection in equids worldwide. Most EIA cases are life-long virus carriers with low antibody reactions and without the appearance of clinical symptoms. A serological test with high sensitivity and specificity is required to detect inapparent infection.
Zhe Hu, Kui Guo, Cheng Du, Jinhui Sun, Ivancho Naletoski, Xiaoyu Chu, Yuezhi Lin, Xuefeng Wang, Maria Barrandeguy, Miño Samuel, Wen Wang, Patrick Imtung Lau, Ulrich Wernery, Rekha Raghavan, Xiaojun Wang   +14 more
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A monoclonal blocking ELISA detecting serum antibodies to Mycoplasma hyopneumoniae

Veterinary Microbiology, 1992
A monoclonal blocking enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to Mycoplasma hyopneumoniae in porcine serum has been developed. The monoclonal antibody (mAb) reacts with an M. hyopneumoniae specific epitope on a molecule of approximately 74 kDa. Only sera from M. hyopneumoniae infected pigs were able to block the binding of
N C, Feld, P, Qvist, P, Ahrens, N F, Friis, A, Meyling   +4 more
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Comparison of blocking dot ELISA and competitive ELISA, using a monoclonal antibody for detection of bluetongue virus antibodies in cattle

Veterinary Microbiology, 1992
A blocking (B) dot enzyme-linked immunosorbent assay (ELISA), using a monoclonal antibody (mAb) against a group specific antigen of bluetongue virus (BTV) is described for the detection of BTV antibodies to BTV in cattle sera. Dots of BTV antigens were adsorbed to nitrocellulose (NC) strips and/or NC mounted in the windows of dipsticks.
A, Afshar, G C, Dulac, J, Riva
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