Results 221 to 230 of about 1,050,605 (265)

A Blocking ELISA for the Detection of Specific Antibodies to Bovine Ephemeral Fever Virus

Journal of Immunological Methods, 1992
A blocking ELISA (B/ELISA) for detecting antibodies to bovine ephemeral fever virus (BEFV) in cattle is described. In this test, the binding capacity of a monoclonal antibody specific for an epitope on antigenic site G1 of the BEF virus glycoprotein is blocked in the presence of positive serum. The sensitivity of the B/ELISA was compared with the virus
Peter Walker, D H Cybinski, H Zakrzewski
exaly   +7 more sources

Blocking-Free ELISA Using a Gold Nanoparticle Layer Coated Commercial Microwell Plate

Sensors, 2018
Enzyme-linked immunosorbent assays (ELISA) show extensive application in immunoassays, to detect and monitor protein biomarkers in clinical diagnosis. Nevertheless, the time required and its multiple steps limit its application.
Songliang He, Ke Zhang, Guoshuai Zhu
exaly   +4 more sources

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A Solid-Phase Blocking ELISA for Detection of Antibodies to Nipah Virus

Journal of Virological Methods, 2004
A monoclonal antibody (MAb) based solid-phase blocking ELISA was developed for detection of antibodies to Nipah virus. The ELISA was designed to detect remaining antigens on the plate with anti-Nipah MAb conjugate after the reaction with sample serum, and enabled simple procedure, detection of neutralizing antibody to Nipah virus, and application of ...
Nobuhiko Tanimura
exaly   +3 more sources

A Liquid-Phase Blocking ELISA for the Detection of Antibodies to Rabies Virus

Journal of Virological Methods, 1995
A liquid-phase blocking ELISA was adapted to the detection and titration of antibodies to principally the nucleoprotein of rabies virus. Sera from animals that had either been vaccinated against rabies or inoculated with street rabies viruses, as well as sera from animals that had no recorded contact with rabies, were tested.
C Préhaud, G R Thomson, G R Thomson
exaly   +3 more sources

Blocking ELISA for Detection of Mumps Virus Antibodies in Human Sera

Journal of Virological Methods, 1993
A new blocking enzyme-linked immunosorbent assay (ELISA) for detection of mumps virus (MuV) specific antibodies in large numbers of human serum samples was developed. The blocking ELISA is based on the reaction of MuV-specific, conjugated monoclonal antibodies (mAbs) with immobilized virus antigen, that has previously been incubated with a two-fold ...
Guy A M Berbers, Pieter van Gageldonk, G A Berbers   +2 more
exaly   +4 more sources

The development and application of a blocking ELISA kit for the diagnosis of infectious coryza

Avian Pathology, 2000
A blocking enzyme-linked immunosorbent assay (B-ELISA) kit for the diagnosis of infectious coryza was developed in this study. The kit was based on a recently described blocking ELISA that uses monoclonal antibodies to achieve specificity for antibodies to either Haemophilus paragallinarum serovar A or serovar C. The results showed that the B-ELISA kit
Miao, D., Zhang, P., Gong, Y., Yamaguchi, T., Iritani, Y., Blackall, P. J.   +5 more
core   +8 more sources

Use of MSG1 Protein in a Novel Blocking ELISA for the Detection of Mycoplasma Suis Infection

Veterinary Journal, 2012
A blocking ELISA based on the monoclonal antibody (MAb) 1A7 was developed to detect antibodies against Mycoplasma suis. The MAb was produced by immunising BALB/c mice with recombinant MSG1 protein (rMSG1) from M. suis expressed in E. coli. Following identification by Western blotting, the MAb was purified and labelled with horseradish peroxidase.
P Jiang
exaly   +3 more sources

Quantitation of the Blocking Effect of Tween 20 and Bovine Serum Albumin in ELISA Microwells

Analytical Biochemistry, 2000
ELISA provides a highly sensitive procedure for quantitating antigens and antibodies. In that assay, microwells are coated initially with a specific ligand and then saturated with inert molecules to minimize nonspecific background. Coating can be improved by pretreating the microwells with poly-l-lysine (PLL).
Michael Steinitz
exaly   +3 more sources

Comparing Electron Microscopy and a Competitive Blocking ELISA in the Detection of Rotaviruses in Porcine Faeces

Veterinary Journal, 2011
Analysis of 476 faecal samples from diarrhoeic piglets was performed using electron microscopy (EM) and a competitive blocking (CB)-ELISA based on monoclonal antibodies to the VP6 protein of group A rotavirus. Rotavirus was detected by EM and/or CB-ELISA in 111 (23.3%) samples.
Ladislav, Rodák, Radka, Smítalová, Bedřich, Šmíd, Zora, Nevoránková, Lubomír, Valíček, Ivan, Pšikal   +5 more
exaly   +3 more sources