Results 11 to 20 of about 1,441,912 (353)

Evaluation of a new combined Western and line blot assay (EUROLINE-WB) for diagnosis and species identification of Echinococcus infection in humans

GMS Infectious Diseases, 2019
Serological detection of echinococcosis is crucial for diagnosis and management. We evaluated the new blot assay Euroline-WB (ELB, Euroimmun) which consists of a Western blot with vesicle antigens and a line blot part with recombinant antigens from ...
Deininger, Susanne, Wellinghausen, Nele
doaj   +1 more source

Western Blot

Definitions, 2019
Theoretical Background/Context A blot, in molecular biology, is the technique that involves the transfer of DNA, RNA and proteins from a gel to a membrane (nitrocellulose or PVDF). Proteins can be detected by western blot (immunoblot).
Western Blot
semanticscholar   +1 more source

Aptamer-Based Western Blot for Selective Protein Recognition

Frontiers in Chemistry, 2020
Selective protein recognition is critical in molecular biology techniques such as Western blotting and ELISA. Successful detection of the target proteins in these methods relies on the specific interaction of the antibodies, which often bring a high ...
Yao Wang, Zhe Li, Hanyang Yu
semanticscholar   +1 more source

Northern Blotting [PDF]

, 2013
To measure the size and amount of RNA transcribed from a specific gene of interest.
Shan L, He, Rachel, Green
openaire   +2 more sources

Misleading Westerns: Common Quantification Mistakes in Western Blot Densitometry and Proposed Corrective Measures

BioMed Research International, 2019
Densitometry data generated for Western blots are commonly used to compare protein abundance between samples. In the last decade, it has become apparent that assumptions underpinning these comparisons are often violated in studies reporting Western blot ...
Trent A Butler, J. Paul, E. Chan, Roger Smith, J. Tolosa   +4 more
semanticscholar   +1 more source

Antibody validation for Western blot: By the user, for the user

Journal of Biological Chemistry, 2019
Well-characterized antibody reagents play a key role in the reproducibility of research findings, and inconsistent antibody performance leads to variability in Western blotting and other immunoassays.
Lakshmi Pillai-Kastoori, S. Heaton, Steve D. Shiflett, Annabelle C. Roberts, A. Solache, A. Schutz-Geschwender   +5 more
semanticscholar   +1 more source

In Situ Single-Cell Western Blot on Adherent Cell Culture.

Angewandte Chemie, 2019
Integrating 2D culture of adherent mammalian cells with single-cell western blotting (in situ scWB) uses microfluidic design to eliminate trypsin release of cells to suspension, prior to single-cell isolation and protein analysis.
Yizhe Zhang, I. Naguro, A. Herr
semanticscholar   +1 more source

Northern blotting [PDF]

Proceedings of the Nutrition Society, 1996
Northern blotting is the main method for examining the expression of genes through measurement of an mRNA. Tissue specificity and the factors which regulate expression can both be determined by Northern blotting. A cDNA radioactively labelled with 32P is the most-commonly-used hybridization probe.
openaire   +2 more sources

Les cromlechs de Sohandi (Compte - rendu de fouilles). [PDF]

Munibe Antropologia-Arkeologia, 1984
Se da cuenta de los resultados obtenidos durante la excavación de los cromlechs de Sohandi, que parecen indicar una pervivencia de este rito funerario en la Edad Media.
Jacques Blot
doaj  

Le cromlech Hegieder 7 (compte-rendu de fouille de sauvetage d'urgence). [PDF]

Munibe Antropologia-Arkeologia, 1994
Este círculo de piedras, de 5 metros de diámetro, a 820 m. de altitud, estaba formado por bloques de pudinga, de diferentes tamaños, dispuesto sin orden visible.
Jacques Blot
doaj