Results 1 to 10 of about 98,102 (238)

Protocol to detect m22G modification on single tRNA iso-decoders by immuno-northern blotting [PDF]

STAR Protocols
Summary: tRNA modifications are highly dynamic and play crucial roles in regulating gene expression. Here, we present an assay for analyzing N2, N2-dimethylguanosine (m22G) modifications on tRNAs using an antibody-based detection approach.
Sseu-Pei Hwang, Katherine Barondeau, Catherine Denicourt   +2 more
doaj   +2 more sources

Protocol to analyze tRNA and rRNA processing using biotin-labeled probes [PDF]

STAR Protocols
Summary: Mature tRNAs and rRNAs are derived from larger precursor transcripts through intricate endo- and exonuclease processing steps. Here, we present a protocol to analyze rRNA and tRNA processing using total RNA extracted from human cells.
Sseu-Pei Hwang, Katherine Barondeau, Catherine Denicourt   +2 more
doaj   +2 more sources

Sirtuin 2 inhibitor AGK2 exerts antiviral effects by inducing epigenetic suppression of hepatitis B virus covalently closed circular DNA through recruitment of repressive histone lysine methyltransferases and reduction of cccDNA [PDF]

Frontiers in Cellular and Infection Microbiology
Chronic hepatitis B virus (HBV) infection continues to be a global health concern because current treatments such as interferon-α and nucleos(t)ide analogs cannot fully eliminate the virus due to persistence of covalently closed circular DNA (cccDNA) and
Jumi Kim, Jumi Kim, Jiseon Ha, Jiseon Ha, Chanho Song, Chanho Song, Muhammad Azhar Sajjad, Muhammad Azhar Sajjad, Fadia Kalsoom, Hyeonjoong Kwon, Hyeonjoong Kwon, Jaewoo Park, Sun Park, Sun Park, Kyongmin Kim, Kyongmin Kim   +15 more
doaj   +2 more sources

Validation and characterization of murine gammaherpesvirus 68 antisense transcripts by northern blot analysis and quantitative reverse transcription-PCR [PDF]

Archives of Biological Sciences, 2023
The transcription of mammalian genomes exhibits an intriguing complexity and numerous novel RNA molecules have been identified. Viruses with large DNA genomes, especially herpesviruses, generate many different RNA species, including long non-coding RNAs (
Kara Mehmet
doaj   +1 more source

Targeted knock-down of miR21 primary transcripts using snoMEN vectors induces apoptosis in human cancer cell lines [PDF]

, 2015
We have previously reported an antisense technology, 'snoMEN vectors', for targeted knock-down of protein coding mRNAs using human snoRNAs manipulated to contain short regions of sequence complementarity with the mRNA target. Here we characterise the use
A Kozomara, A Rodriguez, AG Matera, AK Leung, AM Burroughs, AM Cheng, Angus I. Lamond, B Bai, C Ender, Christophe Antoniewski, CL Ahlenstiel, D De Paula, D Iliopoulos, Dalila Bensaddek, EC Lai, EC Lai, EJ Chapman, EJ Jung, Fabio Avolio, FJ Sheedy, FM Boisvert, FM Boisvert, G Reimer, GS Pall, I Rebbapragada, J Elmen, J Krutzfeldt, J Liu, J Weischenfeldt, JC Politz, JS Andersen, JS Andersen, K Yamada, Kayo Yamada, KK Farh, L Agranat, L Trinkle-Mulcahy, LB Frankel, LB Weinstein, M Negrini, M Ono, M Ono, M Ono, M Scherr, ME Hatley, Motoharu Ono, MS Scott, MS Scott, N Bushati, O Isken, RS Coffin, S Baskerville, S Griffiths-Jones, SD Selcuklu, T Kiss, Vackar Afzal, Y Ahmad, YK Kim, YS Lee, YW Lam   +59 more
core   +18 more sources

Immuno-Northern Blotting: Detection of RNA Modifications by Using Antibodies against Modified Nucleosides. [PDF]

PLoS ONE, 2015
The biological roles of RNA modifications are still largely not understood. Thus, developing a method for detecting RNA modifications is important for further clarification.
Eikan Mishima, Daisuke Jinno, Yasutoshi Akiyama, Kunihiko Itoh, Shinnosuke Nankumo, Hisato Shima, Koichi Kikuchi, Yoichi Takeuchi, Alaa Elkordy, Takehiro Suzuki, Kuniyasu Niizuma, Sadayoshi Ito, Yoshihisa Tomioka, Takaaki Abe   +13 more
doaj   +1 more source

A Comprehensive Analysis of Northern versus Liquid Hybridization Assays for mRNAs, Small RNAs, and miRNAs Using a Non-Radiolabeled Approach

Current Issues in Molecular Biology, 2021
Northern blotting (NB), a gold standard for RNA detection, has lost its charm due to its hands-on nature, need for good quality RNA, and radioactivity.
Waqar Ahmad, Bushra Gull, Jasmin Baby, Farah Mustafa   +3 more
doaj   +1 more source

Artificial microRNA-Based RNA Interference and Specific Gene Silencing for Developing Insect Resistance in Solanum lycopersicum

Agronomy, 2021
RNA Interference (RNAi), which works against invading nucleic acids or modulates the expression of endogenous genes, is a natural eukaryotic regulating system, and it works by noncoding smaller RNA molecules.
Mohammad Faisal, Eslam M. Abdel-Salam, Abdulrahman A. Alatar   +2 more
doaj   +1 more source

Isolation and detection of small RNA molecules

Plant, Soil and Environment, 2007
The article describes in detail methodical procedures for isolation of small RNA molecules, their electrophoresis, northern blotting and finally detection by hybridization with radioactively labeled riboprobes.
J. Fulneček
doaj   +1 more source

Human fibroblast interferon RNA transcripts of different sizes in poly(I).poly(C) induced cells [PDF]

, 1983
A
Content, Jean, De Wit, Lucas, Fiers, Walter, Tavernier, Jan   +3 more
core   +2 more sources