Primary culture and mRNA analysis of human ovarian cells
Biological Procedures Online, 2002 Established cell lines are invaluable for studying cell and molecular biological questions. A variety of human ovarian cancer (OC) cell lines exist, however, most have acquired significant genetic alterations from their cells of origin, including ...
Dunfield Lesley D. +2 more
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Polyclonal antibodies against potato spindle tuber viroid RNA
Frontiers in Bioscience-Elite, 2022 Ribonucleic acid (RNA) can act as a hapten in the direct immunization of animals. For antigen synthesis, 65 mg of viroid RNA were obtained by in vitro transcription of the recombinant DNA. We received a reasonable immune response in mice and rabbits with
Yuri Drygin +2 more
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Discovery Of Ethanol-Responsive Small Rnas In Zymomonas Mobilis [PDF]
, 2014 Zymomonas mobilis is a bacterium that can produce ethanol by fermentation. Due to its unique metabolism and efficient ethanol production, Z. mobilis has attracted special interest for biofuel energy applications; an important area of study is the ...
Cho, Seung Hee +3 more
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cDNA-RNA subtractive hybridization reveals increased expression of mycocerosic acid synthase in intracellular Mycobacterium bovis BCG. [PDF]
, 2001 Identifying genes that are differentially expressed by Mycobacterium bovis BCG after phagocytosis by macrophages will facilitate the understanding of the molecular mechanisms of host cell-intracellular pathogen interactions. To identify such genes a cDNA-
Centre Gunnels Wood +7 more
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Northern Blotting of RNA Denatured in Glyoxal Without Buffer Recirculation
BioTechniques, 1997 A rapid and easy procedure for preparing Northern blots is described. RNA is denatured with glyoxal in the presence of ethidium bromide and glycerol, then electrophoresed through agarose in a buffer that does not require recirculation.
William V. Burnett
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Role of the heat shock transcription factor, Hsf1, in a major fungal pathogen that is obligately associated with warm-blooded animals [PDF]
, 2009 Peer reviewedPublisher ...
Brown, Alistair J. P. +3 more
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Identification of small RNAs abundant in Burkholderia cenocepacia biofilms reveal putative regulators with a potential role in carbon and iron metabolism [PDF]
, 2017 Small RNAs play a regulatory role in many central metabolic processes of bacteria, as well as in developmental processes such as biofilm formation. Small RNAs of Burkholderia cenocepacia, an opportunistic pathogenic beta-proteobacterium, are to date not ...
Coenye, Tom +2 more
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Highly Sensitive Northern Hybridization Using a Rapid Protocol for Downward Alkaline Blotting of RNA
BioTechniques, 1998 A simple and fast RNA gel blot procedureis described that uses 50 mM NaOH tosimultaneously transfer and fix RNA to apositively charged nylon membrane. TheRNA is transferred in a downward direction,and transfer is routinely completedwithin 2.5 h.
Ivan L. Ingelbrecht +2 more
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Regulation of transcription elongation in response to osmostress [PDF]
, 1967 Cells trigger massive changes in gene expression upon environmental fluctuations. The Hog1 stress-activated protein kinase (SAPK) is an important regulator of the transcriptional activation program that maximizes cell fitness when yeast cells are exposed
Begley, Victoria Sarah +7 more
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Biological characterization of coronavirus noncanonical transcripts in vitro and in vivo
Virology Journal, 2023 Background In addition to the well-known coronavirus genomes and subgenomic mRNAs, the existence of other coronavirus RNA species, which are collectively referred to as noncanonical transcripts, has been suggested; however, their biological ...
Ching-Hung Lin +10 more
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