Results 51 to 60 of about 57,891 (288)

MOLECULAR PATHOBIOLOGICAL AND SCANNING ELECTRON MICROSCOPIC CHANGES IN HORSE TENDON CELLS TREA TED WITH ENROFLOXACIN

open access: yesPakistan Veterinary Journal, 2003
Fluoroquinolone (FQNL) antibiotics have been used widely in horses because of their broad-spectrum bactericidal activity and relative safety, however, their use is not without risk.
A. Khan1 and J. Halper
doaj  

Verification of differential gene transcription using virtual northern blotting [PDF]

open access: yes, 1999
We introduce here an alternative to conventional northern blotting that requires only minute amounts of RNA. This has been achieved by modification of methods currently used for the mapping of mRNA 5′-terminal ends.
Oliver Franz   +2 more
core  

Accelerated Western Blotting Lab for Biochemistry I Laboratory Curriculum [PDF]

open access: yes, 2020
The purpose of this current study was to design a Western Blotting procedure for the Biochemistry Laboratory curriculum at NMU. The methodology consists of gel electrophoresis, transfer of proteins to a membrane surface, and then a series of ...
Hayes, Katie
core  

Multidimensional Cellular Micro‐Compartments to Model Invasive Lobular Carcinoma Dormancy

open access: yesAdvanced Healthcare Materials, EarlyView.
Invasive lobular carcinoma (ILC) is an understudied subtype of breast cancer that is susceptible to late recurrences. In this study, micro‐compartmentalization techniques spanning multiple dimensions, including 2D, pseudo‐3D, and 3D, are integrated to uncover the mechanisms underlying ILC dormancy, revealing the central role of p27Kip1.
Xilal Y. Rima   +15 more
wiley   +1 more source

Identification and characterization of a novel group of natural anti-sense transcripts from RNA1.2 gene locus of human cytomegalovirus

open access: yesChinese Medical Journal, 2019
. Background:. Natural anti-sense transcripts (NATs), which are transcribed from the complementary DNA strand of annotated genes, exert regulatory function of gene expression.
Bing-Yang Liu   +7 more
doaj   +1 more source

Gellan Gum Fluid Gel System for Controlled‐Delivery of Cytokine‐Licensed MSC‐EVs to Enhance Corneal Repair in Limbal Stem Cell Deficiency

open access: yesAdvanced Healthcare Materials, EarlyView.
Gellan gum fluid gel (FG) enables controlled ocular delivery of mesenchymal stromal cell (MSC)‐derived extracellular vesicles (EV). Its shear‐thinning, solid‐liquid‐solid rheology ensures prolonged ocular retention. Sustained MSC‐EV release (∼18% in 6 hrs) supports effective EV ocular delivery.
Seyedmohammad Moosavizadeh   +7 more
wiley   +1 more source

Nucleic acid blotting : the basics / [PDF]

open access: yes, 1994
Nucleic acid hybridization techniques allow the detection of specific DNA or RNA sequences. This book is a clear and concise guide to the techniques used for preparing DNA and RNA for membrane hybridization.
Darling, David C.,(viaf)24698035   +1 more
core  

Intimate Interaction Between Nucleic Acid and Conjugated Polymers in Organic Electrochemical Transistors Enables Ultrasensitive Biomarker Detection

open access: yesAdvanced Materials, EarlyView.
The intimate interaction between negatively charged RNA molecules and conjugated polymers in organic electrochemical transistors (OECTs) in aqueous electrolytes is investigated. It demonstrates that RNA binding reduces the volumetric capacitance of the polymer channel, enabling the development of an ultrasensitive biosensing platform capable of ...
Hong Liu   +7 more
wiley   +1 more source

Stimulation of ZPC Production by Follicle-Stimulating Hormone in the Granulosa Cells of Japanese Quail (Coturnix Japonica)

open access: yesThe Journal of Poultry Science, 2003
Avian perivitelline membrane, an investment homologous to the zona pellucida of mammalian oocytes, is composed of two glycoproteins. Previous studies have indicated that one of the components, a glycoprotein homologous to mammalian ZPC, is produced and ...
Jianzhi Pan   +2 more
doaj   +1 more source

Genome‐Wide CRISPR Screen Identifies a microRNA Orchestrating Pleiotropic Resistance to Targeted Therapy and T Cell Immunity in Melanoma

open access: yesAdvanced Science, EarlyView.
A genome‐wide microRNA CRISPR screen identifies miR‐18a as a master regulator of cross‐resistance in melanoma. Loss of miR‐18a activates the AJUBA–YAP/Hippo axis to confer BRAFi resistance and enhances THBS1–CD47 interaction to impair CD8+ T cell immunity. hnRNP A1 is identified as an upstream regulator of miR‐18a processing.
Zhao Wang   +19 more
wiley   +1 more source

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