Results 181 to 190 of about 21,819 (222)

CLOSTRIDIUM BOTULINUM TYPE E TOXIN AND TOXOID

Canadian Journal of Microbiology, 1954
A strain of Clostridium botulinum Type E in a beef heart infusion – peptone broth yields moderate amounts of toxin when grown at 30 °C. but fails to produce toxin at 37°. When grown in a cellophane sac suspended in a similar medium, at 30 °C, there is a 5- to 10-fold increase in the yield of toxin.
A L, BARRON, G B, REED
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Ozone Inactivation of Botulinum Type E Toxin

Ozone: Science & Engineering, 1984
Abstract The supernatant toxin of Clostridium botulinum type E, at a concentration of 2x104 mouse units (MU/ml), was exposed to ozone at a flow rate of 110 ml/minute (8.5 ppm dissolved residual), and was inactivated after twelve minutes. A tenfold lower concentration was completely inactivated in less than one minute with an ozone concentration of 0.01
John T. Graikoski, Walter J. Blogoslawski, Joseph Choromanski   +2 more
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Repression of toxin production by tryptophan in Clostridium botulinum type E

Archives of Microbiology, 1990
Of the seven amino acids required by Clostridium botulinum type E, tryptophan is the most essential and may provide the cell with nitrogen. The addition of excess tryptophan (10-20 mM) or other nitrogenous nutrients to minimal growth medium markedly decreased toxin formation but did not affect growth in C. botulinum type E.
G J, Leyer, E A, Johnson
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Purification and some properties of Clostridium botulinum type-E toxin

Biochimica et Biophysica Acta (BBA) - Protein Structure, 1968
Abstract To investigate the mechanism of the activation of Clostridium botulinum type-E toxin, attempts were made to improve our previous procedures for purification of the precursor by introducing a step of percolation of the cell extract through a column of CM-Sephadex.
M, Kitamura, S, Sakaguchi, G, Sakaguchi
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Chromatographic purification of the toxin of Type E Clostridiumbotulinum

Biochemical and Biophysical Research Communications, 1969
Abstract Clostridium botulinum Type E extracellular toxin was purified on DEAE cellulose columns following ammonium sulfate precipitation and dialysis. Two fractions, one toxic and one non-toxic, were obtained. Disc electrophoresis of the toxic fraction showed three bands, two of which were not toxic.
A S, Emodi, R V, Lechowich
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Dissociation and reconstitution of 12-S toxin of Clostridium botulinum type E

Biochimica et Biophysica Acta (BBA) - Protein Structure, 1969
Abstract 1. 1. Dissociation of prototoxin and activated 12-S toxin of Clostridium botulinum type E under alkaline conditions may occur in two steps: molecular unfolding and molecular division. The molecular unfolding reduces the sedimentation coefficient from 11.6 S to 10.3 S; the molecular division produces the toxic Eα and nontoxic Eβ ...
M, Kitamura, G, Sakaguchi
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A common subunit structure in Clostridium botulinum type A, B and E toxins

Biochemical and Biophysical Research Communications, 1972
Abstract Tryptic activation of Clostridium botulinum type E progenitor toxin of 147,000 mol. wt. involves cleavage of the molecule into at least two polypeptides that are separable when the disulfide bond(s) linking them is reduced. The 50,000 and 102,000 mol. wt. of these chains compare with the 53,000 and 97,000 values of the disulfide-linked
B R, DasGupta, H, Sugiyama
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Growth and Toxin Production of Clostridium botulinum type E

Journal of Applied Bacteriology, 1968
SUMMARY Growth and toxin production of Clostridium botulinum type E was studied at 37, 30, 22 and 4°. Incubation at 30° yielded maximum numbers of viable cells but the toxicity was not different from cultures grown at 22°, thus indicating that the toxin synthesis/cell was ...
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Mechanism of Tryptic Activation ofClostridium botulinumType E Toxin

Journal of Bacteriology, 1965
Gerwing, Julia(University of British Columbia, Vancouver, B.C., Canada),Claude E. Dolman, and Arthur Ko. Mechanism of tryptic activation ofClostridium botulinumtype E toxin. J. Bacteriol.89:1176–1179. 1965.—The toxic peptide of trypsin activatedClostridium botulinumtype E toxin was purified by chromatography through columns packed with Sephadex G-75 ...
J, GERWING, C E, DOLMAN, A, KO
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THE EFFECT OF TEMPERATURE ON TOXIN FORMATION AND TOXIN STABILITY OF CLOSTRIDIUM BOTULINUM TYPE E IN DIFFERENT ENVIRONMENTS

Canadian Journal of Microbiology, 1966
Clostridium botulinum type E was found to produce toxin at temperatures between 3° and 30 °C in Robertson"s chopped meat medium and in fish dialyzate. Toxin was produced more rapidly in the meat medium. No toxin was found after 1 year of incubation at 1 °C.
K, Abrahamsson, B, Gullmar, N, Molin
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