Results 91 to 100 of about 2,145,263 (295)

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

open access: yesFEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour   +2 more
wiley   +1 more source

Dose‐dependent hepatotoxicity of hydrogen peroxide in HepG2 cells and its modulation by CYP450 induction

open access: yesFEBS Open Bio, EarlyView.
NMR metabolomics revealed concentration‐dependent metabolic perturbations in HepG2 cells exposed to H2O2. Rifampicin pretreatment enhanced metabolic competence, attenuated toxin‐induced alterations and produced metabolite profiles more consistent with human liver physiology, supporting the use of CYP450‐induced HepG2 models for improved in vitro ...
Maren Jinks   +4 more
wiley   +1 more source

Bitter gourd, Momordica charantia L., breeding lines differ in secondary metabolite content according to market type

open access: yesJournal of Applied Botany and Food Quality, 2019
Bitter gourd, Momordica charantia L., is an important commercial cucurbitaceous vegetable of enormous medicinal value in Asia because of its secondary metabolite content.
Bernhard Trierweiler   +8 more
doaj   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

open access: yesFEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty   +13 more
wiley   +1 more source

BREEDING FORAGE-GRAIN CULTURES IN THE VOLGA REGION

open access: yesВ мире научных открытий, 2016
With the aim of creating varieties forage-grain directions for a ten years, conducted a comprehensive assessment of 80 lines of grain sorghum, 55 lines of sweet sorghum, 70 lines of Sudan grass and 15 varieties and lines of foxtail millet.
Elena Aleksandrovna Vertikova
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

CGIAR Excellence in Breeding Platform - Plan of Work and Budget 2021

open access: yes, 2021
The CGIAR Excellence in Breeding (EiB) Platform is accelerating the modernization of crop breeding programs that serve farmers in low- and middle-income countries.
CGIAR Excellence in Breeding Platform
core  

ЕФЕКТ НА РАЗЛИЧНИ ИЗТОЧНИЦИ НА СПЕЦИФИЧЕН ВАРИАНС ВЪРХУ ТЕГЛОВНОТО РАЗВИТИЕ И ВЪЛНОДАЙНАТА ПРОДУКТИВНОСТ НА ОВЦЕ ОТ КАРАКАЧАНСКАТА ПОРОДА

open access: yesЖивотновъдни науки, 2014
An investigation with 314 sheep from the Karakachan breed during the period 2003-2010, originated from Kabiuk herd, Shumen was carried out. Parameters live weight at weaning, 9 months, 1.5 years and 2.5 years of age, wool productivity traits - quantity ...
ГЕНОВЕВА СТАЙКОВА   +1 more
doaj  

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

open access: yesFEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti   +5 more
wiley   +1 more source

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

open access: yesFEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta   +7 more
wiley   +1 more source

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