Results 51 to 60 of about 5,575 (204)

Thiotemplated Polyketide Chain Fusion and Reductive Cyclization Build the Reactive Butenolide Core of Malleicyprol

Angewandte Chemie, Volume 137, Issue 31, July 28, 2025.
Malleicyprol, a virulence factor of notorious animal and human pathogens of the Burkholderia pseudomallei (BP) group, results from the fusion of two individual polyketide chains. The noncanonical condensation domain of the PKS–NRPS hybrid synthetase BurF forms an ester bond, and the terminal reductase domain mediates a reductive chain release followed ...
Jonas Fiedler, Leo Dumjahn, Mie Ishida‐Ito, Felix Trottmann, Keishi Ishida, Christian Hertweck   +5 more
wiley   +2 more sources

DBSecSys 2.0: a database of Burkholderia mallei and Burkholderia pseudomallei secretion systems. [PDF]

BMC Bioinformatics, 2016
Abstract Background Burkholderia mallei and B. pseudomallei are the causative agents of glanders and melioidosis, respectively, diseases with high morbidity and mortality rates. B. mallei and B. pseudomallei are closely related genetically; B. mallei evolved from an ancestral strain of B. pseudomallei by genome reduction
Memišević V, Kumar K, Zavaljevski N, DeShazer D, Wallqvist A, Reifman J.   +5 more
europepmc   +3 more sources

Multiplex qPCR for reliable detection and differentiation of Burkholderia mallei and Burkholderia pseudomallei

BMC Infectious Diseases, 2013
Background Burkholderia mallei and B. pseudomallei are two closely related species of highly virulent bacteria that can be difficult to detect. Pathogenic Burkholderia are endemic in many regions worldwide and cases of infection, sometimes brought by ...
Janse Ingmar, Hamidjaja Raditijo A, Hendriks Amber CA, van Rotterdam Bart J   +3 more
doaj   +1 more source

Development of Real-Time Multiplex PCR Assay for the Detection and Differentiation of Burkholderia mallei and Burkholderia pseudomallei

Проблемы особо опасных инфекций, 2016
Objective of the study was to develop a real-time multiplex PCR assay for the detection and differentiation of B. mallei and B. pseudomallei, characterized by high sensitivity and specificity. Materials and Methods.
L. V. Lemasova, G. A. Tkachenko, S. S. Savchenko, O. S. Bondareva, V. A. Antonov   +4 more
doaj   +1 more source

Use of a safe, reproducible, and rapid aerosol delivery method to study infection by Burkholderia pseudomallei and Burkholderia mallei in mice. [PDF]

PLoS ONE, 2013
Burkholderia pseudomallei, the etiologic agent of melioidosis, is a saprophytic bacterium readily isolated from wet soils of countries bordering the equator. Burkholderia mallei is a host-adapted clone of B.
Eric R Lafontaine, Shawn M Zimmerman, Teresa L Shaffer, Frank Michel, Xiudan Gao, Robert J Hogan   +5 more
doaj   +1 more source

Burkholderia Hep_Hag autotransporter (BuHA) proteins elicit a strong antibody response during experimental glanders but not human melioidosis

BMC Microbiology, 2007
Background The bacterial biothreat agents Burkholderia mallei and Burkholderia pseudomallei are the cause of glanders and melioidosis, respectively. Genomic and epidemiological studies have shown that B.
Foster Simon J, Clarke Simon R, Rengpipat Sirirat, Tumapa Sarinna, Holden Matthew TG, Tiyawisutsri Rachaneeporn, Nierman William C, Day Nicholas PJ, Peacock Sharon J   +8 more
doaj   +1 more source

Whole-genome sequencing of a quarter-century melioidosis outbreak in temperate Australia uncovers a region of low-prevalence endemicity [PDF]

, 2016
This study was funded by the National Health and Medical Research Council via awards 1046812 and 1098337, and the Wellcome Trust Sanger Institute via award 098051. S.J.P.
Buller, Nicky, Chapple, Stephanie N. J., Currie, Bart J, Golledge, Clayton, Holden, Matthew T. G., Mayo, Mark, Peacock, Sharon J., Price, Erin P., Sarovich, Derek S.   +8 more
core   +1 more source

Production of Monoclonal Antibodies against Outer Membrane Proteins of Burkholderia pseudomallei, Strain C-141

Проблемы особо опасных инфекций, 2008
Monoclonal antibodies (MAb) were produced against two B. pseudomallei high-purified membrane proteins with Mr 29 kDa (p29) and 45 kDa (p45). Monoclonal antibodies from culture supernatant fluids of 4F2 and 1G11 clones showed specific interaction with ...
S. S. Vetchinin, P. C. Kopylov, N. V. Kiseleva, A. M. Baranov, E. V. Baranova, E. V. Galkina, A. I. Borzilov, I. Y. Kalachev   +7 more
doaj   +1 more source

Natural Burkholderia mallei Infection in Dromedary, Bahrain

Emerging Infectious Diseases, 2011
We confirm a natural infection of dromedaries with glanders. Multilocus variable number tandem repeat analysis of a Burkholderia mallei strain isolated from a diseased dromedary in Bahrain revealed close genetic proximity to strain Dubai 7, which caused an outbreak of glanders in horses in the United Arab Emirates in 2004.
Ulrich Wernery, Renate Wernery, Marina Joseph, Fajer Al-Salloom, Bobby Johnson, Joerg Kinne, Shanti Jose, Sherry Jose, Britta Tappendorf, Heidie Hornstra, Holger Scholz   +10 more
openaire   +3 more sources

Clear distinction between Burkholderia mallei and Burkholderia pseudomallei using fluorescent motB primers [PDF]

Acta Veterinaria Scandinavica, 2015
A frame-shift mutation in the flagellum motor gene motB coding for the chemotaxis MotB protein of Burkholderia mallei has been utilized to design a conventional duplex PCR assay with fluorescent labelled primers.Species specificity was tested with a panel of 13 Burkholderia type strains. A total of 41 B. mallei field strains, 36 B.
Schmoock, Gernot, Elschner, Mandy, Sprague, Lisa D   +2 more
openaire   +2 more sources