Results 51 to 60 of about 5,430 (204)

microarray probe design for diagnosis of multiple pathogens [PDF]

open access: yes, 2008
Background With multiple strains of various pathogens being sequenced, it is necessary to develop high-throughput methods that can simultaneously process multiple bacterial or viral genomes to find common fingerprints as well as fingerprints that are ...
Ravi Vijaya Satya   +7 more
core   +2 more sources

Multiplex qPCR for reliable detection and differentiation of Burkholderia mallei and Burkholderia pseudomallei

open access: yesBMC Infectious Diseases, 2013
Background Burkholderia mallei and B. pseudomallei are two closely related species of highly virulent bacteria that can be difficult to detect. Pathogenic Burkholderia are endemic in many regions worldwide and cases of infection, sometimes brought by ...
Janse Ingmar   +3 more
doaj   +1 more source

Development of Real-Time Multiplex PCR Assay for the Detection and Differentiation of Burkholderia mallei and Burkholderia pseudomallei

open access: yesПроблемы особо опасных инфекций, 2016
Objective of the study was to develop a real-time multiplex PCR assay for the detection and differentiation of B. mallei and B. pseudomallei, characterized by high sensitivity and specificity. Materials and Methods.
L. V. Lemasova   +4 more
doaj   +1 more source

Use of a safe, reproducible, and rapid aerosol delivery method to study infection by Burkholderia pseudomallei and Burkholderia mallei in mice. [PDF]

open access: yesPLoS ONE, 2013
Burkholderia pseudomallei, the etiologic agent of melioidosis, is a saprophytic bacterium readily isolated from wet soils of countries bordering the equator. Burkholderia mallei is a host-adapted clone of B.
Eric R Lafontaine   +5 more
doaj   +1 more source

DBSecSys 2.0: a database of Burkholderia mallei and Burkholderia pseudomallei secretion systems. [PDF]

open access: yesBMC Bioinformatics, 2016
Abstract Background Burkholderia mallei and B. pseudomallei are the causative agents of glanders and melioidosis, respectively, diseases with high morbidity and mortality rates. B. mallei and B. pseudomallei are closely related genetically; B. mallei evolved from an ancestral strain of B. pseudomallei by genome reduction
Memišević V   +5 more
europepmc   +3 more sources

Burkholderia Hep_Hag autotransporter (BuHA) proteins elicit a strong antibody response during experimental glanders but not human melioidosis

open access: yesBMC Microbiology, 2007
Background The bacterial biothreat agents Burkholderia mallei and Burkholderia pseudomallei are the cause of glanders and melioidosis, respectively. Genomic and epidemiological studies have shown that B.
Foster Simon J   +8 more
doaj   +1 more source

Production of Monoclonal Antibodies against Outer Membrane Proteins of Burkholderia pseudomallei, Strain C-141

open access: yesПроблемы особо опасных инфекций, 2008
Monoclonal antibodies (MAb) were produced against two B. pseudomallei high-purified membrane proteins with Mr 29 kDa (p29) and 45 kDa (p45). Monoclonal antibodies from culture supernatant fluids of 4F2 and 1G11 clones showed specific interaction with ...
S. S. Vetchinin   +7 more
doaj   +1 more source

Natural Burkholderia mallei Infection in Dromedary, Bahrain

open access: yesEmerging Infectious Diseases, 2011
We confirm a natural infection of dromedaries with glanders. Multilocus variable number tandem repeat analysis of a Burkholderia mallei strain isolated from a diseased dromedary in Bahrain revealed close genetic proximity to strain Dubai 7, which caused an outbreak of glanders in horses in the United Arab Emirates in 2004.
Ulrich Wernery   +10 more
openaire   +3 more sources

Myd88-dependent recruitment of monocytes and dendritic cells required for protection from pulmonary Burkholderia mallei infection [PDF]

open access: yes, 2012
The Gram-negative bacterium Burkholderia mallei causes rapidly fatal illness in equines and humans when contracted by inhalation and also has the potential to be used as a bioweapon.
Goodyear, Andrew   +3 more
core   +1 more source

Clear distinction between Burkholderia mallei and Burkholderia pseudomallei using fluorescent motB primers [PDF]

open access: yesActa Veterinaria Scandinavica, 2015
A frame-shift mutation in the flagellum motor gene motB coding for the chemotaxis MotB protein of Burkholderia mallei has been utilized to design a conventional duplex PCR assay with fluorescent labelled primers.Species specificity was tested with a panel of 13 Burkholderia type strains. A total of 41 B. mallei field strains, 36 B.
Schmoock, Gernot   +2 more
openaire   +2 more sources

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