Results 51 to 60 of about 302,602 (263)
Structural insights into an engineered feruloyl esterase with improved MHET degrading properties
FEBS Letters, EarlyView.A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa +5 more
wiley +1 more source
Structural Determinants of Raft Partitioning for Single-Pass Transmembrane Proteins [PDF]
Biophysical Journal, 2016 Transmembrane proteins (TMPs) comprise ∼30% of the mammalian proteome and mediate nearly all functions of the cell. The subcellular localization, trafficking, signalling, and enzymatic activity of many TMPs is regulated by their association with lipid-driven lateral membrane domains known as lipid rafts.
Lorent, Joseph H. +3 more
openaire +1 more source
FEBS Letters, EarlyView.Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura +2 more
wiley +1 more source
254 Markers of Neurodegeneration Associate with Worse Performance of Complex Daily Tasks
Journal of Clinical and Translational Science, 2022 OBJECTIVES/GOALS: In preclinical Alzheimer disease (AD), biomarkers such as amyloid protein, decreased hippocampal volume, and weaker network connections are apparent in the brain, but memory symptoms assessed by paper and pencil tests have not yet ...
Audrey A. Keleman +3 more
doaj +1 more source
An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane
FEBS Letters, EarlyView.Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa +3 more
wiley +1 more source
pH‐mediated activation of the lysosomal arginine sensor SLC38A9
FEBS Letters, EarlyView.Cells monitor nutrient levels via the lysosomal transporter SLC38A9 to activate the mechanistic target of rapamycin complex 1 (mTORC1). This study reveals that SLC38A9 function is regulated by pH. We identified histidine 544 as a critical pH sensor that undergoes conformational changes to control amino acid efflux from lysosomes; therefore, it ...
Xuelang Mu, Ampon Sae Her, Tamir Gonen
wiley +1 more source
Pull in and Push Out: Mechanisms of Horizontal Gene Transfer in Bacteria
Frontiers in Microbiology, 2018 Horizontal gene transfer (HGT) plays an important role in bacterial evolution. It is well accepted that DNA is pulled/pushed into recipient cells by conserved membrane-associated DNA transport systems, which allow the entry of only single-stranded DNA ...
Dongchang Sun
doaj +1 more source
Degradation mechanism of the von Willebrand factor A2 domain by nattokinase
FEBS Letters, EarlyView.Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto +3 more
wiley +1 more source
Kinetics-like equation for cerebrospinal fluid-blood diffusion models
Revista Cubana de Investigaciones Biomédicas, 2020 Introduction: The diffusion of proteins from the blood to the cerebrospinal fluid is influenced by its molecular weight and by the intrinsic properties and biological properties of the protein. Objective: Determinate the diffusion of new proteins as they
José Alejandro Rodríguez-Pérez +6 more
doaj
PASsing a signal: low carbs, less protein.
Chemistry & biology, 2003 AbstractPAS kinases combine the sensor functions of PAS (Per-Arnt-Sim) domains with the regulatory phosphorylation activity of protein kinases. Complementary multidisciplinary reports reveal coordinated regulation of sugar storage and protein synthesis by PAS kinase and suggest structural coupling of ligand and kinase binding by the PAS domain.
openaire +2 more sources