Results 51 to 60 of about 17,188 (276)

Detection of c-di-GMP-Responsive DNA Binding [PDF]

, 2017
Modulation of signal transduction via binding of the secondary messenger molecule cyclic di-GMP to effector proteins is a near universal regulatory schema in bacteria. In particular, direct binding of c-di-GMP to transcriptional regulators has been shown to alter gene expression of a variety of processes.
Jacob R. Chambers, Karin Sauer
openaire   +2 more sources

A Multimodal Strategy Used By A Large c-di-GMP Network [PDF]

Journal of Bacteriology, 2017
AbstractThePseudomonas fluorescensgenome encodes for 50+ proteins involved in -di-GMP signaling. Here, we demonstrate that when tested across 188 nutrients, these enzymes and effectors appear capable of impacting biofilm formation. Transcriptional analysis of network members across ∼50 nutrient conditions indicates that altered gene expression can ...
Dahlstrom, Kurt M., Collins, Alan J., Doing, Georgia, Taroni, Jacyln N, Gauvin, Timothy J., Greene, Casey S., Hogan, Deborah A., O’Toole, George A.   +7 more
openaire   +4 more sources

More than Enzymes That Make or Break Cyclic Di-GMP—Local Signaling in the Interactome of GGDEF/EAL Domain Proteins of Escherichia coli

mBio, 2017
The bacterial second messenger bis-(3′-5′)-cyclic diguanosine monophosphate (c-di-GMP) ubiquitously promotes bacterial biofilm formation. Intracellular pools of c-di-GMP seem to be dynamically negotiated by diguanylate cyclases (DGCs, with GGDEF domains)
Olga Sarenko, Gisela Klauck, Franziska M. Wilke, Vanessa Pfiffer, Anja M. Richter, Susanne Herbst, Volkhard Kaever, Regine Hengge   +7 more
doaj   +1 more source

Microbial exopolysaccharide production by polyextremophiles in the adaptation to multiple extremes

FEBS Letters, EarlyView.
Polyextremophiles are microorganisms that endure multiple extreme conditions by various adaptation strategies that also include the production of exopolysaccharides (EPSs). This review provides an integrated perspective on EPS biosynthesis, function, and regulation in these organisms, emphasizing their critical role in survival and highlighting their ...
Tracey M Gloster, Ebru Toksoy Öner
wiley   +1 more source

Elevated intracellular cyclic‐di‐GMP level in Shewanella oneidensis increases expression of c‐type cytochromes

Microbial Biotechnology, 2020
Summary Electrochemically active biofilms are capable of exchanging electrons with solid electron acceptors and have many energy and environmental applications such as bioelectricity generation and environmental remediation.
Chun Kiat Ng, Jiabao Xu, Zhao Cai, Liang Yang, Ian P. Thompson, Wei E. Huang, Bin Cao   +6 more
doaj   +1 more source

A nucleotide‐independent, pan‐RAS‐targeted DARPin elicits anti‐tumor activity in a multimodal manner

Molecular Oncology, EarlyView.
We report a Designed Ankyrin Repeat Protein that binds and inhibits RAS proteins, which serve as central cell signaling hubs and are essential for the progression of many cancers. Its unique feature is that it does not discriminate between different RAS isoforms or mutations and is capable of binding to RAS in both its active (GTP‐bound) and inactive ...
Jonas N. Kapp, Wouter P. R. Verdurmen, Jonas V. Schaefer, Kari Kopra, Gabriela Nagy‐Davidescu, Elodie Richard, Marie‐Julie Nokin, Patrick Ernst, Rastislav Tamaskovic, Martin Schwill, Ralph Degen, Claudia Scholl, David Santamaria, Andreas Plückthun   +13 more
wiley   +1 more source

A Visible Light‐Responsive Hydrogel to Study the Effect of Dynamic Tissue Stiffness on Cellular Mechanosensing

Advanced Functional Materials, EarlyView.
A visible light‐responsive polyacrylamide‐azobenzene hydrogel enables safe, reversible stiffness control for studying cell mechanobiology without harmful UV exposure. This approach reveals stem cells respond rapidly to mechanical changes, showing altered shape and protein distribution within one hour.
Aafreen Ansari, Sanchyan Bhowmik, Kaiwen Zhang, Chayla L. Reeves, Danielle Vahala, Yu Suk Choi, Amy Gelmi, Alexander N. Combes, Rocky S. Tuan, Vinh X. Truong, John S. Forsythe, Jessica E. Frith   +11 more
wiley   +1 more source

Plasma‐Treated Hydrogel for Combined RONS and Chemotherapy Delivery: A Proof‐of‐Concept In Ovo

Advanced Functional Materials, EarlyView.
This study explores plasma‐treated hydrogels (PTH) as a new way to deliver both reactive oxygen and nitrogen species and chemotherapy (Doxorubicin) directly to tumors. In ovo tests show effects after a single dose, especially in osteosarcoma tumors. Tumor weight decrease, and a resistance‐related protein is reduced.
Milica Živanić, Albert Espona‐Noguera, Angela Privat Maldonado, Patrik Matušů, Francesco Tampieri, Maria‐Pau Ginebra, Abraham Lin, Evelien Smits, Annemie Bogaerts, Cristina Canal   +9 more
wiley   +1 more source

Pleiotropic Effects of c-di-GMP Content in Pseudomonas syringae [PDF]

Applied and Environmental Microbiology, 2019
The present work comprehensively analyzed the transcriptome and phenotypes that were regulated by c-di-GMP in P. syringae . Given that the majority of diguanylate cyclases and phosphodiesterases have not been characterized in P.
Stephan C. Schuster, Xin Deng, Liang Yang, Liang Yang, Weitong Zhang, Canfeng Hua, Yingchao Zhang, Yingpeng Xie, Zhao Cai, Tingting Wang, Xiaolong Shao   +10 more
openaire   +4 more sources

Precise Quantification of Angiogenesis in 3D Biomaterials Using in Vitro CAM Models: Enhancing 3Rs in Research and Minimizing in Vivo Dependency

Advanced Functional Materials, EarlyView.
The study develops elastin–fibrin scaffolds and evaluates angiogenesis using the chorioallantoic membrane (CAM) model. Advanced imaging with confocal microscopy and Indocyanine Green dye enables precise 3D quantification of vascular networks. Findings demonstrate superior microvessel visualization compared to micro‐CT using Microfil and Optiray 350 ...
Ece Melis Er, Martha Patricia Pérez González, Beatriz Gil Garrido, Jasmine Ho, Hae‐Won Kim, Jonathan Campbell Knowles, Jia Hua, Ferdinand Lali, Prasad Sawadkar   +8 more
wiley   +1 more source