Results 81 to 90 of about 459,122 (273)

Delivery of Human iPSC‐Derived RPE Cells in Healthy Minipig Retina Results in Interaction Between Photoreceptors and Transplanted Cells

Advanced Science, EarlyView.
Human induced pluripotent stem cell‐derived retinal pigment epithelial (hiPSC‐RPE) cells, cultured on a clinically relevant PDLLA scaffold, are transplanted into immunosuppressed minipigs. The study demonstrates the viability, integration, and preservation of RPE‐specific features without gliosis or neuroinflammation, supporting their potential as a ...
Anna Macečková Brymová, Francisco Javier Rodriguez‐Jimenez, Annika Konrad, Yaroslav Nemesh, Muhammed Arshad Thottappali, Ana Artero‐Castro, Ruslan Nyshchuk, Anastasiia Kolesnikova, Brigitte Müller, Hana Studenovska, Jana Juhasova, Stefan Juhas, Ivona Valekova, Dunja Lukovic, Claudia Aleman, Taras Ardan, Saskia Drutovič, Jan Motlik, Zdenka Ellederova, Zbinek Straňák, Miroslav Veith, Lyubomyr Lytvynchuk, Ruchi Sharma, Kapil Bharti, Goran Petrovski, Pavla Jendelova, Knut Stieger, Slaven Erceg   +27 more
wiley   +1 more source

The Streptomyces master regulator BldD binds c-di-GMP sequentially to create a functional BldD2-(c-di-GMP)4 complex

Nucleic Acids Research, 2017
Streptomyces are ubiquitous soil bacteria that undergo a complex developmental transition coinciding with their production of antibiotics. This transition is controlled by binding of a novel tetrameric form of the second messenger, 3΄-5΄ cyclic diguanylic acid (c-di-GMP) to the master repressor, BldD.
Maria A. Schumacher, Natalia Tschowri, Wenjie Zeng, Kim Findlay, Mark J. Buttner, Richard G. Brennan   +5 more
openaire   +3 more sources

Therapeutic Delivery of circDYM by Perillyl Alcohol Nanoemulsion Alleviates LPS‐Induced Depressive‐Like Behaviors

Advanced Science, EarlyView.
PANEs are specifically designed to enhance the brain delivery efficiency of circDYM compared to commercialized lipid nanoparticles (LNPs). Intranasal administration of PANE‐circDYM effectively alleviated LPS‐induced depressive‐like behaviors by mitigating neuroinflammation and promoting neuroplasticity.
Feng Gao, Zhongkun Zhang, Minzi Ju, Liang Bian, Huijuan Wang, Sibo Zhao, Ningbo Cai, Yu Wang, Yanpeng Jia, Ling Shen, Yuan Zhang, Honghong Yao   +11 more
wiley   +1 more source

The Characterization of a Cyclic-Di-GMP (c-Di-GMP) Pathway Leads to a New Tool for Studying c-Di-GMP Metabolic Genes [PDF]

Journal of Bacteriology, 2013
Second messengers play an integral role in regulating a wide variety of pathways in response to triggers from the extracellular milieu. The intracellular second messenger cyclic diguanylate (c-di-GMP) has been shown to regulate microbial adaptation to extracellular environments in myriad ways.
openaire   +3 more sources

Nuclear Translocation of S100A9 Triggers Senescence of Human Amnion Fibroblasts by De‐Repressing LINE1 Via Heterochromatin Erosion at Parturition

Advanced Science, EarlyView.
This study shows that the classical secretory protein S100 calcium‐binding protein A9 (S100A9) can translocate to the nucleus upon de‐phosphorylation at Thr 113 in human amnion fibroblasts at parturition, where S100A9 induces heterochromatin erosion through segregation of the heterochromatin maintenance protein, resulting inLong Interspersed Nuclear ...
Fan Zhang, Meng‐Die Li, Fan Pan, Wen‐Jia Lei, Yang Xi, Li‐Jun Ling, Leslie Myatt, Kang Sun, Wang‐Sheng Wang   +8 more
wiley   +1 more source

DNA–Protein Binding is Dominated by Short Anchoring Elements

Advanced Science, EarlyView.
This study delineate the binding mechanism of transcription factors (TFs) in the genome with the knowledge obtained at thermodynamic equilibrium conditions. Through KaScape experiments, combined with the AEEscape algorithm development, they identified the minimal dominant interacting elements, termed as Anchoring Elements (AEs).
Hong Chen, Yongping Xu, Hao Ge, Xiao‐Dong Su   +3 more
wiley   +1 more source

Neuronal C/EBPβ Shortens the Lifespan via Inactivating NAMPT

Advanced Science, EarlyView.
Schematic diagram illustrating the mechanism of shortened lifespan in Thy 1‐C/EBPβ transgenic mice and intervention strategies. Briefly, C/EBPβ/AEP escalation in the aging brain triggers NAMPT fragmentation and NAD+ depletion. Knockout of AEP from Thy 1‐C/EBPβ Tg/Tg mice ameliorates cognitive impairments and elongates the lifespan.
Bowei Li, Zhongyun Xie, Mengmeng Wang, Shuke Nie, Zhengjiang Qian, Xin Meng, Xia Liu, Seong Su Kang, Keqiang Ye   +8 more
wiley   +1 more source

Antigen targeting to dendritic cells: Still a place in future immunotherapy?

European Journal of Immunology, Volume 52, Issue 12, Page 1909-1924, December 2022., 2022
Dendritic cell‐based immunotherapy is a promising vaccination strategy to treat infectious diseases, autoimmunity, and cancer. In this review, we present an overview on the current state of antigen targeting approaches via C‐type lectin receptors, with an outlook on future developments and potential for clinical translation.
Patrizia Stoitzner, Nikolaus Romani, Christoph Rademacher, Hans Christian Probst, Karsten Mahnke   +4 more
wiley   +1 more source

Nanoneedle‐Based Electroporation for Efficient Manufacturing of Human Primary Chimeric Antigen Receptor Regulatory T‐Cells

Advanced Science, EarlyView.
Low reproducibility and inefficient gene transfer hamper clinical manufacturing of chimeric antigen receptor regulatory T cells (CAR Tregs). Nanoneedle mediated electroporation generates primary human CAR Tregs under good manufacturing practice conditions with superior efficiency compared to viral transduction.
Ningjia Sun, Cong Wang, William Edwards, Yikai Wang, Xiangrong L. Lu, Chenlei Gu, Samuel McLennan, Panicos Shangaris, Peng Qi, Daniela Mastronicola, Cristiano Scottà, Giovanna Lombardi, Ciro Chiappini   +12 more
wiley   +1 more source

Dendritic cells generated from induced pluripotent stem cells and by direct reprogramming of somatic cells

European Journal of Immunology, Volume 52, Issue 12, Page 1880-1888, December 2022., 2022
Somatic cells (fibroblasts, dendritic cells, T cells) are reprogrammed into induced pluripotent stem cells (iPS cells) with specific transcription factors. iPS cells are then differentiated into iPS cell‐derived DC (ipDC) with DC inducing cytokines. Fibroblasts are directly reprogrammed into induced DC (iDC) by DC specific transcription factors (direct
Niclas Flosdorf, Martin Zenke
wiley   +1 more source